Title: Natural deep eutectic solvent mediated extrusion for continuous high-solid pretreatment of lignocellulosic biomass

Abstract

Several deep eutectic solvents (DESs) have been demonstrated to be highly effective for lignocellulosic biomass pretreatment, combining the advantages of simple synthesis, relatively low chemical cost and better biocompatibility. However, low biomass loading that is usually involved with DES pretreatment hinders its practical use. Here, a twin-screw extruder was used for pretreating biomass sorghum bagasse at solid loadings up to 50%, mediated by a neutral-pH DES, choline chloride : glycerol (ChCl : Gly). This continuous extrusion process led to high glucose and xylose yields of >85% from enzymatic saccharification of the pretreated sorghum. A combination of microscopic, spectroscopic, and X-ray diffraction analyses demonstrate a high degree of defibration and disruption of the biomass cell wall structures; however, little or no change in chemical compositions. Further results from gel permeation chromatographic (GPC) and nuclear magnetic resonance (NMR) spectroscopic analyses indicate that ChCl : Gly-mediated extrusion preserved the basic lignin structural characteristics with no significant differences between extruded biomass at a solid loading of 30% and 50%. This study demonstrates the potential of DES-mediated extrusion as a highly effective continuous high-solid biomass pretreatment technology for industrially relevant applications.

Authors:

Ai, Binling  ^[1];

• Search DOE PAGES for author "Ai, Binling"
• Search DOE PAGES for ORCID "0000-0003-0996-7608"

Li, Wenqi  ^[2];

• Search DOE PAGES for author "Li, Wenqi"
• Search DOE PAGES for ORCID "0000-0003-1246-6919"

Woomer, Joseph  ^[2];

• Search DOE PAGES for author "Woomer, Joseph"
• Search DOE PAGES for ORCID "0000-0001-9499-6563"

Li, Mi ^[3]; Pu, Yunqiao  ^[4];

• Search DOE PAGES for author "Pu, Yunqiao"
• Search DOE PAGES for ORCID "0000-0003-2554-1447"

Sheng, Zhanwu ^[5]; Zheng, Lili ^[5]; Adedeji, Akinbode ^[2]; Ragauskas, Arthur J.  ^[6];

• Search DOE PAGES for author "Ragauskas, Arthur J."
• Search DOE PAGES for ORCID "0000-0002-3536-554X"

Shi, Jian  ^[2]

• Search DOE PAGES for author "Shi, Jian"
• Search DOE PAGES for ORCID "0000-0003-3022-4446"

---

+ Show Author Affiliations

1. Chinese Academy of Tropical Agricultural Sciences, Haikou (China). Haikou Experimental Station; Univ. of Kentucky, Lexington, KY (United States). Biosystems and Agricultural Engineering
2. Univ. of Kentucky, Lexington, KY (United States). Biosystems and Agricultural Engineering
3. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon
4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JICS), Center for BioEnergy Innovation, Biosciences Division
5. Chinese Academy of Tropical Agricultural Sciences, Haikou (China). Haikou Experimental Station
6. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon and Dept. of Chemical and Biomolecular Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JICS), Center for BioEnergy Innovation, Biosciences Division

Publication Date:

Tue Jul 07 00:00:00 EDT 2020

Research Org.:

Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER); USDA; National Natural Science Foundation of China (NSFC); Chinese Scholarship Council; Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences

OSTI Identifier:

1651282

Alternate Identifier(s):

OSTI ID: 1642252

Grant/Contract Number:  

AC05-00OR22725; 1015068; 1018315; 51708548; 201803260012; 17CXTD-05

Resource Type:

Accepted Manuscript

Journal Name:

Green Chemistry

Additional Journal Information:

Journal Volume: 22; Journal Issue: 16; Journal ID: ISSN 1463-9262

Publisher:

Royal Society of Chemistry

Country of Publication:

United States

Language:

English

Subject:

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY