Sphingolipid domains in the plasma membranes of fibroblasts are not enriched with cholesterol
Abstract
The plasma membranes of mammalian cells are widely expected to contain domains that are enriched with cholesterol and sphingolipids. In this work, we have used high-resolution secondary ion mass spectrometry to directly map the distributions of isotope-labeled cholesterol and sphingolipids in the plasma membranes of intact fibroblast cells. Although acute cholesterol depletion reduced sphingolipid domain abundance, cholesterol was evenly distributed throughout the plasma membrane and was not enriched within the sphingolipid domains. As a result, we rule out favorable cholesterol-sphingolipid interactions as dictating plasma membrane organization in fibroblast cells. Because the sphingolipid domains are disrupted by drugs that depolymerize the cells actin cytoskeleton, cholesterol must instead affect the sphingolipid organization via an indirect mechanism that involves the cytoskeleton.
- Authors:
-
- Univ. of Illinois, Urbana, IL (United States)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- National Institutes of Health, Bethesda, MD (United States)
- Univ. of Illinois, Urbana, IL (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1313553
- Report Number(s):
- LLNL-JRNL-638518
Journal ID: ISSN 0021-9258
- Grant/Contract Number:
- AC52-07NA27344
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Biological Chemistry
- Additional Journal Information:
- Journal Volume: 288; Journal Issue: 23; Journal ID: ISSN 0021-9258
- Publisher:
- American Society for Biochemistry and Molecular Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; cholesterol; isotopic tracers; lipid raft; mass spectrometry (MS); membrane structure; plasma membrane; sphingolipid
Citation Formats
Frisz, Jessica F., Klitzing, Haley A., Lou, Kaiyan, Hutcheon, Ian D., Weber, Peter K., Zimmerberg, Joshua, and Kraft, Mary L. Sphingolipid domains in the plasma membranes of fibroblasts are not enriched with cholesterol. United States: N. p., 2013.
Web. doi:10.1074/jbc.M113.473207.
Frisz, Jessica F., Klitzing, Haley A., Lou, Kaiyan, Hutcheon, Ian D., Weber, Peter K., Zimmerberg, Joshua, & Kraft, Mary L. Sphingolipid domains in the plasma membranes of fibroblasts are not enriched with cholesterol. United States. https://doi.org/10.1074/jbc.M113.473207
Frisz, Jessica F., Klitzing, Haley A., Lou, Kaiyan, Hutcheon, Ian D., Weber, Peter K., Zimmerberg, Joshua, and Kraft, Mary L. Mon .
"Sphingolipid domains in the plasma membranes of fibroblasts are not enriched with cholesterol". United States. https://doi.org/10.1074/jbc.M113.473207. https://www.osti.gov/servlets/purl/1313553.
@article{osti_1313553,
title = {Sphingolipid domains in the plasma membranes of fibroblasts are not enriched with cholesterol},
author = {Frisz, Jessica F. and Klitzing, Haley A. and Lou, Kaiyan and Hutcheon, Ian D. and Weber, Peter K. and Zimmerberg, Joshua and Kraft, Mary L.},
abstractNote = {The plasma membranes of mammalian cells are widely expected to contain domains that are enriched with cholesterol and sphingolipids. In this work, we have used high-resolution secondary ion mass spectrometry to directly map the distributions of isotope-labeled cholesterol and sphingolipids in the plasma membranes of intact fibroblast cells. Although acute cholesterol depletion reduced sphingolipid domain abundance, cholesterol was evenly distributed throughout the plasma membrane and was not enriched within the sphingolipid domains. As a result, we rule out favorable cholesterol-sphingolipid interactions as dictating plasma membrane organization in fibroblast cells. Because the sphingolipid domains are disrupted by drugs that depolymerize the cells actin cytoskeleton, cholesterol must instead affect the sphingolipid organization via an indirect mechanism that involves the cytoskeleton.},
doi = {10.1074/jbc.M113.473207},
journal = {Journal of Biological Chemistry},
number = 23,
volume = 288,
place = {United States},
year = {Mon Apr 22 00:00:00 EDT 2013},
month = {Mon Apr 22 00:00:00 EDT 2013}
}
Web of Science
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