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Title: Hemagglutinin Clusters in the Plasma Membrane Are Not Enriched with Cholesterol and Sphingolipids

The clusters of the influenza envelope protein, hemagglutinin, within the plasma membrane are hypothesized to be enriched with cholesterol and sphingolipids. Here in this paper, we directly tested this hypothesis by using high-resolution secondary ion mass spectrometry to image the distributions of antibody-labeled hemagglutinin and isotope-labeled cholesterol and sphingolipids in the plasma membranes of fibroblast cells that stably express hemagglutinin. We found that the hemagglutinin clusters were neither enriched with cholesterol nor colocalized with sphingolipid domains. Thus, hemagglutinin clustering and localization in the plasma membrane is not controlled by cohesive interactions between hemagglutinin and liquid-ordered domains enriched with cholesterol and sphingolipids, or from specific binding interactions between hemagglutinin, cholesterol, and/or the majority of sphingolipid species in the plasma membrane.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [4]
  1. Univ. of Illinois, Urbana, IL (United States). Dept. of Chemistry
  2. National Inst. of Health (NIH), Bethesda, MD (United States). Eunice Kennedy Shriver National Inst. of Child Health and Human Development, Section on Cellular and Membrane Biophysics
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Glenn T. Seaborg Inst.
  4. Univ. of Illinois, Urbana, IL (United States). Dept. of Chemistry; Univ. of Illinois, Urbana, IL (United States). Dept. of Chemical and Biomolecular Engineering
Publication Date:
Report Number(s):
LLNL-JRNL-698175
Journal ID: ISSN 0006-3495
Grant/Contract Number:
AC52-07NA27344
Type:
Published Article
Journal Name:
Biophysical Journal
Additional Journal Information:
Journal Volume: 108; Journal Issue: 7; Journal ID: ISSN 0006-3495
Publisher:
Elsevier
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE; National Science Foundation (NSF); National Institutes of Health (NIH)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY
OSTI Identifier:
1246558
Alternate Identifier(s):
OSTI ID: 1396214