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Title: Cholesterol is enriched in the sphingolipid patches on the substrate near nonpolarized MDCK cells, but not in the sphingolipid domains in their plasma membranes

Abstract

In this paper, information about the distributions of cholesterol and sphingolipids within the plasma membranes of mammalian cells provides insight into the roles of these molecules in membrane function. In this report, high-resolution secondary ion mass spectrometry was used to image the distributions of metabolically incorporated rare isotope-labeled sphingolipids and cholesterol on the surfaces of nonpolarized epithelial cells. Sphingolipid domains that were not enriched with cholesterol were detected in the plasma membranes of subconfluent Madin-Darby canine kidney cells. Surprisingly, cholesterol-enriched sphingolipid patches were observed on the substrate adjacent to these cells. Based on the shapes of these cholesterol-enriched sphingolipid patches on the substrate and their proximity to cellular projections, we hypothesize that they are deposits of membranous particles released by the cell.

Authors:
; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1567872
Alternate Identifier(s):
OSTI ID: 1476856
Report Number(s):
LLNL-JRNL-745101
Journal ID: ISSN 0005-2736; S0005273618301263; PII: S0005273618301263
Grant/Contract Number:  
08-LW-015; AC52-07NA27344
Resource Type:
Published Article
Journal Name:
Biochimica et Biophysica Acta. Biomembranes
Additional Journal Information:
Journal Name: Biochimica et Biophysica Acta. Biomembranes Journal Volume: 1860 Journal Issue: 10; Journal ID: ISSN 0005-2736
Publisher:
Elsevier
Country of Publication:
Netherlands
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Sphingolipids; Cholesterol; Madin-Darby canine kidney cells; Lipid domains; Secondary ion mass spectrometry imaging; Stable isotope labeling

Citation Formats

Yeager, Ashley N., Weber, Peter K., and Kraft, Mary L. Cholesterol is enriched in the sphingolipid patches on the substrate near nonpolarized MDCK cells, but not in the sphingolipid domains in their plasma membranes. Netherlands: N. p., 2018. Web. doi:10.1016/j.bbamem.2018.04.008.
Yeager, Ashley N., Weber, Peter K., & Kraft, Mary L. Cholesterol is enriched in the sphingolipid patches on the substrate near nonpolarized MDCK cells, but not in the sphingolipid domains in their plasma membranes. Netherlands. doi:10.1016/j.bbamem.2018.04.008.
Yeager, Ashley N., Weber, Peter K., and Kraft, Mary L. Mon . "Cholesterol is enriched in the sphingolipid patches on the substrate near nonpolarized MDCK cells, but not in the sphingolipid domains in their plasma membranes". Netherlands. doi:10.1016/j.bbamem.2018.04.008.
@article{osti_1567872,
title = {Cholesterol is enriched in the sphingolipid patches on the substrate near nonpolarized MDCK cells, but not in the sphingolipid domains in their plasma membranes},
author = {Yeager, Ashley N. and Weber, Peter K. and Kraft, Mary L.},
abstractNote = {In this paper, information about the distributions of cholesterol and sphingolipids within the plasma membranes of mammalian cells provides insight into the roles of these molecules in membrane function. In this report, high-resolution secondary ion mass spectrometry was used to image the distributions of metabolically incorporated rare isotope-labeled sphingolipids and cholesterol on the surfaces of nonpolarized epithelial cells. Sphingolipid domains that were not enriched with cholesterol were detected in the plasma membranes of subconfluent Madin-Darby canine kidney cells. Surprisingly, cholesterol-enriched sphingolipid patches were observed on the substrate adjacent to these cells. Based on the shapes of these cholesterol-enriched sphingolipid patches on the substrate and their proximity to cellular projections, we hypothesize that they are deposits of membranous particles released by the cell.},
doi = {10.1016/j.bbamem.2018.04.008},
journal = {Biochimica et Biophysica Acta. Biomembranes},
number = 10,
volume = 1860,
place = {Netherlands},
year = {2018},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1016/j.bbamem.2018.04.008

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Cited by: 1 work
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