Proton mediated control of biochemical reactions with bioelectronic pH modulation
Abstract
In Nature, protons (H+) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase, and voltage-regulated H+ channels activating bioluminescence in firefly and jellyfish. Artificial devices that control H+ currents and H+ concentration (pH) are able to actively influence biochemical processes. Here, we demonstrate a biotransducer that monitors and actively regulates pH-responsive enzymatic reactions by monitoring and controlling the flow of H+ between PdHx contacts and solution. The present transducer records bistable pH modulation from an “enzymatic flip-flop” circuit that comprises glucose dehydrogenase and alcohol dehydrogenase. Furthermore, the transducer also controls bioluminescence from firefly luciferase by affecting solution pH.
- Authors:
-
- Univ. of Washington, Seattle, WA (United States)
- Univ. of Washington, Seattle, WA (United States); Univ. of California, Santa Cruz, CA (United States)
- Publication Date:
- Research Org.:
- Univ. of Washington, Seattle, WA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1253913
- Grant/Contract Number:
- SC0010441
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 6; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biomedical engineering; electrical and electronic engineering
Citation Formats
Deng, Yingxin, Miyake, Takeo, Keene, Scott, Josberger, Erik E., and Rolandi, Marco. Proton mediated control of biochemical reactions with bioelectronic pH modulation. United States: N. p., 2016.
Web. doi:10.1038/srep24080.
Deng, Yingxin, Miyake, Takeo, Keene, Scott, Josberger, Erik E., & Rolandi, Marco. Proton mediated control of biochemical reactions with bioelectronic pH modulation. United States. https://doi.org/10.1038/srep24080
Deng, Yingxin, Miyake, Takeo, Keene, Scott, Josberger, Erik E., and Rolandi, Marco. Thu .
"Proton mediated control of biochemical reactions with bioelectronic pH modulation". United States. https://doi.org/10.1038/srep24080. https://www.osti.gov/servlets/purl/1253913.
@article{osti_1253913,
title = {Proton mediated control of biochemical reactions with bioelectronic pH modulation},
author = {Deng, Yingxin and Miyake, Takeo and Keene, Scott and Josberger, Erik E. and Rolandi, Marco},
abstractNote = {In Nature, protons (H+) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase, and voltage-regulated H+ channels activating bioluminescence in firefly and jellyfish. Artificial devices that control H+ currents and H+ concentration (pH) are able to actively influence biochemical processes. Here, we demonstrate a biotransducer that monitors and actively regulates pH-responsive enzymatic reactions by monitoring and controlling the flow of H+ between PdHx contacts and solution. The present transducer records bistable pH modulation from an “enzymatic flip-flop” circuit that comprises glucose dehydrogenase and alcohol dehydrogenase. Furthermore, the transducer also controls bioluminescence from firefly luciferase by affecting solution pH.},
doi = {10.1038/srep24080},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {Thu Apr 07 00:00:00 EDT 2016},
month = {Thu Apr 07 00:00:00 EDT 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 19 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Protons are a neurotransmitter that regulates synaptic plasticity in the lateral amygdala
journal, June 2014
- Du, J.; Reznikov, L. R.; Price, M. P.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 24
Proton Acts as a Neurotransmitter for Nicotine-Induced Adrenergic and Calcitonin Gene-Related Peptide-Containing Nerve-Mediated Vasodilation in the Rat Mesenteric Artery
journal, May 2009
- Kawasaki, Hiromu; Eguchi, Shinji; Miyashita, Satoko
- Journal of Pharmacology and Experimental Therapeutics, Vol. 330, Issue 3
Voltage-gated proton channels: what's next?: Voltage-gated proton channels
journal, November 2008
- DeCoursey, Thomas E.
- The Journal of Physiology, Vol. 586, Issue 22
Enzymatic biofuel cells designed for direct power generation from biofluids in living organisms
journal, January 2011
- Miyake, Takeo; Haneda, Keigo; Nagai, Nobuhiro
- Energy & Environmental Science, Vol. 4, Issue 12
High-performance transistors for bioelectronics through tuning of channel thickness
journal, May 2015
- Rivnay, Jonathan; Leleux, Pierre; Ferro, Marc
- Science Advances, Vol. 1, Issue 4
An enzyme logic bioprotonic transducer
journal, January 2015
- Miyake, Takeo; Josberger, Erik E.; Keene, Scott
- APL Materials, Vol. 3, Issue 1
Electrochemistry in Diabetes Management
journal, July 2010
- Heller, Adam; Feldman, Ben
- Accounts of Chemical Research, Vol. 43, Issue 7
Molecularly Ordered Bioelectrocatalytic Composite Inside a Film of Aligned Carbon Nanotubes
journal, August 2012
- Yoshino, Syuhei; Miyake, Takeo; Yamada, Takeo
- Advanced Energy Materials, Vol. 3, Issue 1
The Rise of Organic Bioelectronics
journal, September 2013
- Rivnay, Jonathan; Owens, Róisín M.; Malliaras, George G.
- Chemistry of Materials, Vol. 26, Issue 1
Organic bioelectronics for electronic-to-chemical translation in modulation of neuronal signaling and machine-to-brain interfacing
journal, September 2013
- Larsson, Karin C.; Kjäll, Peter; Richter-Dahlfors, Agneta
- Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1830, Issue 9, p. 4334-4344
New opportunities for organic electronics and bioelectronics: ions in action
journal, January 2013
- Tarabella, Giuseppe; Mahvash Mohammadi, Farzaneh; Coppedè, Nicola
- Chemical Science, Vol. 4, Issue 4
Organic materials for printed electronics
journal, January 2007
- Berggren, M.; Nilsson, D.; Robinson, N. D.
- Nature Materials, Vol. 6, Issue 1
Nanostraws for Direct Fluidic Intracellular Access
journal, December 2011
- VanDersarl, Jules J.; Xu, Alexander M.; Melosh, Nicholas A.
- Nano Letters, Vol. 12, Issue 8
Stochastic transport through carbon nanotubes in lipid bilayers and live cell membranes
journal, October 2014
- Geng, Jia; Kim, Kyunghoon; Zhang, Jianfei
- Nature, Vol. 514, Issue 7524
Programmable transdermal drug delivery of nicotine using carbon nanotube membranes
journal, June 2010
- Wu, J.; Paudel, K. S.; Strasinger, C.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 26
Voltage-Gated Proton Channels: Molecular Biology, Physiology, and Pathophysiology of the H V Family
journal, April 2013
- DeCoursey, Thomas E.
- Physiological Reviews, Vol. 93, Issue 2
A polysaccharide bioprotonic field-effect transistor
journal, September 2011
- Zhong, Chao; Deng, Yingxin; Roudsari, Anita Fadavi
- Nature Communications, Vol. 2, Issue 1
H+-type and OH−-type biological protonic semiconductors and complementary devices
journal, October 2013
- Deng, Yingxin; Josberger, Erik; Jin, Jungho
- Scientific Reports, Vol. 3, Issue 1
Two-Terminal Protonic Devices with Synaptic-Like Short-Term Depression and Device Memory
journal, May 2014
- Josberger, Erik E.; Deng, Yingxin; Sun, Wei
- Advanced Materials, Vol. 26, Issue 29
Bulk protonic conductivity in a cephalopod structural protein
journal, June 2014
- Ordinario, David D.; Phan, Long; Walkup IV, Ward G.
- Nature Chemistry, Vol. 6, Issue 7
Electrode potentials of the palladium + hydrogen system
journal, January 1959
- Flanagan, Ted B.; Lewis, F. A.
- Transactions of the Faraday Society, Vol. 55
Biofuel cell anode: NAD+/glucose dehydrogenase-coimmobilized ketjenblack electrode
journal, September 2009
- Miyake, T.; Oike, M.; Yoshino, S.
- Chemical Physics Letters, Vol. 480, Issue 1-3
Boolean logic gates based on oxygen-controlled biofuel cell in “one pot”
journal, April 2011
- Zhou, Ming; Wang, Fuan; Dong, Shaojun
- Electrochimica Acta, Vol. 56, Issue 11
Bending behavior of Cu-plated Pd–Ni alloys ribbon driven by hydrogenation
journal, August 2009
- Mizumoto, Masayuki; Ohgai, Takeshi; Kagawa, Akio
- Journal of Alloys and Compounds, Vol. 482, Issue 1-2
Fabrication and Characterization of Nanostructured Pd Hydride pH Microelectrodes
journal, January 2006
- Imokawa, Toru; Williams, Kirsty-Jo; Denuault, Guy
- Analytical Chemistry, Vol. 78, Issue 1
Materials for electrochemical capacitors
journal, November 2008
- Simon, Patrice; Gogotsi, Yury
- Nature Materials, Vol. 7, Issue 11
Flexible, layered biofuel cells
journal, February 2013
- Miyake, Takeo; Haneda, Keigo; Yoshino, Syuhei
- Biosensors and Bioelectronics, Vol. 40, Issue 1
The electrochemistry of gold in acid aqueous solutions containing chloride ions
journal, December 1975
- Gallego, J. Herrera; Castellano, C. E.; Calandra, A. J.
- Journal of Electroanalytical Chemistry and Interfacial Electrochemistry, Vol. 66, Issue 3
A set of multicolored Photinus pyralis luciferase mutants for in vivo bioluminescence applications
journal, October 2005
- Shapiro, Elyse; Lu, Connie; Baneyx, François
- Protein Engineering, Design and Selection, Vol. 18, Issue 12
Firefly bioluminescence quantum yield and colour change by pH-sensitive green emission
journal, December 2007
- Ando, Yoriko; Niwa, Kazuki; Yamada, Nobuyuki
- Nature Photonics, Vol. 2, Issue 1
Flexible, layered biofuel cells
journal, February 2013
- Miyake, Takeo; Haneda, Keigo; Yoshino, Syuhei
- Biosensors and Bioelectronics, Vol. 40, Issue 1
Bending behavior of Cu-plated Pd–Ni alloys ribbon driven by hydrogenation
journal, August 2009
- Mizumoto, Masayuki; Ohgai, Takeshi; Kagawa, Akio
- Journal of Alloys and Compounds, Vol. 482, Issue 1-2
Fabrication and Characterization of Nanostructured Pd Hydride pH Microelectrodes
journal, January 2006
- Imokawa, Toru; Williams, Kirsty-Jo; Denuault, Guy
- Analytical Chemistry, Vol. 78, Issue 1
Electrochemistry in Diabetes Management
journal, July 2010
- Heller, Adam; Feldman, Ben
- Accounts of Chemical Research, Vol. 43, Issue 7
Nanostraws for Direct Fluidic Intracellular Access
journal, December 2011
- VanDersarl, Jules J.; Xu, Alexander M.; Melosh, Nicholas A.
- Nano Letters, Vol. 12, Issue 8
A polysaccharide bioprotonic field-effect transistor
journal, September 2011
- Zhong, Chao; Deng, Yingxin; Roudsari, Anita Fadavi
- Nature Communications, Vol. 2, Issue 1
Materials for electrochemical capacitors
journal, November 2008
- Simon, Patrice; Gogotsi, Yury
- Nature Materials, Vol. 7, Issue 11
H+-type and OH−-type biological protonic semiconductors and complementary devices
journal, October 2013
- Deng, Yingxin; Josberger, Erik; Jin, Jungho
- Scientific Reports, Vol. 3, Issue 1
An enzyme logic bioprotonic transducer
journal, January 2015
- Miyake, Takeo; Josberger, Erik E.; Keene, Scott
- APL Materials, Vol. 3, Issue 1
Programmable transdermal drug delivery of nicotine using carbon nanotube membranes
journal, June 2010
- Wu, J.; Paudel, K. S.; Strasinger, C.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 26
Protons are a neurotransmitter that regulates synaptic plasticity in the lateral amygdala
journal, June 2014
- Du, J.; Reznikov, L. R.; Price, M. P.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 24
A set of multicolored Photinus pyralis luciferase mutants for in vivo bioluminescence applications
journal, October 2005
- Shapiro, Elyse; Lu, Connie; Baneyx, François
- Protein Engineering, Design and Selection, Vol. 18, Issue 12
Proton Acts as a Neurotransmitter for Nicotine-Induced Adrenergic and Calcitonin Gene-Related Peptide-Containing Nerve-Mediated Vasodilation in the Rat Mesenteric Artery
journal, May 2009
- Kawasaki, Hiromu; Eguchi, Shinji; Miyashita, Satoko
- Journal of Pharmacology and Experimental Therapeutics, Vol. 330, Issue 3
Voltage-Gated Proton Channels: Molecular Biology, Physiology, and Pathophysiology of the H V Family
journal, April 2013
- DeCoursey, Thomas E.
- Physiological Reviews, Vol. 93, Issue 2
Organic bioelectronics
book, January 2013
- Tarabella, G.; Coppedè, N.; Iannotta, S.
- Handbook of Organic Materials for Optical and (Opto)electronic Devices
High-performance transistors for bioelectronics through tuning of channel thickness.
text, January 2015
- Rivnay, Jonathan; Leleux, Pierre; Ferro, Marc
- Apollo - University of Cambridge Repository
Works referencing / citing this record:
Soft and Ion‐Conducting Materials in Bioelectronics: From Conducting Polymers to Hydrogels
journal, January 2020
- Jia, Manping; Rolandi, Marco
- Advanced Healthcare Materials, Vol. 9, Issue 5
Taking Electrons out of Bioelectronics: From Bioprotonic Transistors to Ion Channels
journal, March 2017
- Strakosas, Xenofon; Selberg, John; Hemmatian, Zahra
- Advanced Science, Vol. 4, Issue 7
A Bioelectronic Platform Modulates pH in Biologically Relevant Conditions
journal, January 2019
- Strakosas, Xenofon; Selberg, John; Zhang, Xiaolin
- Advanced Science, Vol. 6, Issue 7
A protonic biotransducer controlling mitochondrial ATP synthesis
journal, July 2018
- Zhang, Z.; Kashiwagi, H.; Kimura, S.
- Scientific Reports, Vol. 8, Issue 1
A non-enzymatic glucose sensor enabled by bioelectronic pH control
journal, July 2019
- Strakosas, Xenofon; Selberg, John; Pansodtee, Pattawong
- Scientific Reports, Vol. 9, Issue 1
Bio-inspired protonic memristor devices based on metal complexes with proton-coupled electron transfer
journal, January 2019
- Hiruma, Yusuke; Yoshikawa, Kai; Haga, Masa-aki
- Faraday Discussions, Vol. 213
Enhanced protonic conductivity and IFET behavior in individual proton-doped electrospun chitosan fibers
journal, January 2019
- Lee, Woo-Kyung; Pietron, Jeremy J.; Kidwell, David A.
- Journal of Materials Chemistry C, Vol. 7, Issue 35
Proton Conductivity of Glycosaminoglycans
journal, August 2018
- Selberg, John; Jia, Manping; Rolandi, Marco
- PLOS ONE
Proton conductivity of glycosaminoglycans
journal, March 2019
- Selberg, John; Jia, Manping; Rolandi, Marco
- PLOS ONE, Vol. 14, Issue 3
Taking Electrons out of Bioelectronics: From Bioprotonic Transistors to Ion Channels
journal, March 2017
- Strakosas, Xenofon; Selberg, John; Hemmatian, Zahra
- Advanced Science, Vol. 4, Issue 7
Endogenous Bioelectrics in Development, Cancer, and Regeneration: Drugs and Bioelectronic Devices as Electroceuticals for Regenerative Medicine
journal, December 2019
- Levin, Michael; Selberg, John; Rolandi, Marco
- iScience, Vol. 22
A protonic biotransducer controlling mitochondrial ATP synthesis
journal, July 2018
- Zhang, Z.; Kashiwagi, H.; Kimura, S.
- Scientific Reports, Vol. 8, Issue 1
A non-enzymatic glucose sensor enabled by bioelectronic pH control
journal, July 2019
- Strakosas, Xenofon; Selberg, John; Pansodtee, Pattawong
- Scientific Reports, Vol. 9, Issue 1