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Title: An enzyme logic bioprotonic transducer

Abstract

Translating ionic currents into measureable electronic signals is essential for the integration of bioelectronic devices with biological systems. We demonstrate the use of a Pd/PdH{sub x} electrode as a bioprotonic transducer that connects H{sup +} currents in solution into an electronic signal. This transducer exploits the reversible formation of PdH{sub x} in solution according to PdH↔Pd + H{sup +} + e{sup −}, and the dependence of this formation on solution pH and applied potential. We integrate the protonic transducer with glucose dehydrogenase as an enzymatic AND gate for glucose and NAD{sup +}. PdH{sub x} formation and associated electronic current monitors the output drop in pH, thus transducing a biological function into a measurable electronic output.

Authors:
; ; ;  [1];  [1];  [2]
  1. Department of Materials Science and Engineering, University of Washington, Seattle, Washington 98195-2120 (United States)
  2. (United States)
Publication Date:
OSTI Identifier:
22415239
Resource Type:
Journal Article
Resource Relation:
Journal Name: APL materials; Journal Volume: 3; Journal Issue: 1; Other Information: (c) 2014 Author(s); Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; BIOLOGICAL FUNCTIONS; ELECTRIC CURRENTS; ELECTRODES; ENZYMES; GLUCOSE; HYDROGEN IONS 1 PLUS; NAD; PALLADIUM; PALLADIUM HYDRIDES; PH VALUE; POTENTIALS; TRANSDUCERS

Citation Formats

Miyake, Takeo, Keene, Scott, Deng, Yingxin, Rolandi, Marco, E-mail: rolandi@uw.edu, Josberger, Erik E., and Department of Electrical Engineering, University of Washington, Seattle, Washington 98195-2500. An enzyme logic bioprotonic transducer. United States: N. p., 2015. Web. doi:10.1063/1.4900886.
Miyake, Takeo, Keene, Scott, Deng, Yingxin, Rolandi, Marco, E-mail: rolandi@uw.edu, Josberger, Erik E., & Department of Electrical Engineering, University of Washington, Seattle, Washington 98195-2500. An enzyme logic bioprotonic transducer. United States. doi:10.1063/1.4900886.
Miyake, Takeo, Keene, Scott, Deng, Yingxin, Rolandi, Marco, E-mail: rolandi@uw.edu, Josberger, Erik E., and Department of Electrical Engineering, University of Washington, Seattle, Washington 98195-2500. Thu . "An enzyme logic bioprotonic transducer". United States. doi:10.1063/1.4900886.
@article{osti_22415239,
title = {An enzyme logic bioprotonic transducer},
author = {Miyake, Takeo and Keene, Scott and Deng, Yingxin and Rolandi, Marco, E-mail: rolandi@uw.edu and Josberger, Erik E. and Department of Electrical Engineering, University of Washington, Seattle, Washington 98195-2500},
abstractNote = {Translating ionic currents into measureable electronic signals is essential for the integration of bioelectronic devices with biological systems. We demonstrate the use of a Pd/PdH{sub x} electrode as a bioprotonic transducer that connects H{sup +} currents in solution into an electronic signal. This transducer exploits the reversible formation of PdH{sub x} in solution according to PdH↔Pd + H{sup +} + e{sup −}, and the dependence of this formation on solution pH and applied potential. We integrate the protonic transducer with glucose dehydrogenase as an enzymatic AND gate for glucose and NAD{sup +}. PdH{sub x} formation and associated electronic current monitors the output drop in pH, thus transducing a biological function into a measurable electronic output.},
doi = {10.1063/1.4900886},
journal = {APL materials},
number = 1,
volume = 3,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 2015},
month = {Thu Jan 01 00:00:00 EST 2015}
}