Planar 2D wireframe DNA origami
Abstract
Two-dimensional (2D) DNA origami is widely used for applications ranging from excitonics to single-molecule biophysics. Conventional, single-layer 2D DNA origami exhibits flexibility and curvature in solution; however, that may limit its suitability as a 2D structural template. In contrast, 2D wireframe DNA origami rendered with six-helix bundle edges offers local control over duplex orientations with enhanced in-plane rigidity. Here, we investigate the 3D structure of these assemblies using cryo–electron microscopy (cryo-EM). 3D reconstructions reveal a high degree of planarity and homogeneity in solution for polygonal objects with and without internal mesh, enabling 10-Å resolution for a triangle. Coarse-grained simulations were in agreement with cryo-EM data, offering molecular structural insight into this class of 2D DNA origami. Our results suggest that these assemblies may be valuable for 2D material applications and geometries that require high structural fidelity together with local control over duplex orientations, rather than parallel duplex assembly.
- Authors:
-
- Massachusetts Institute of Technology (MIT), Cambridge, MA (United States)
- Stanford University, CA (United States); University of Science and Technology of China, Hefei (China)
- Massachusetts Institute of Technology (MIT), Cambridge, MA (United States); Jeonbuk National University, Jeonju (Korea, Republic of)
- University of Oxford (United Kingdom)
- Stanford University, CA (United States); SLAC National Accelerator Laboratory, Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); US Department of the Navy, Office of Naval Research (ONR); Army Research Office (ARO); Alexander von Humboldt Foundation; Engineering and Physical Sciences Research Council (EPSRC)
- OSTI Identifier:
- 1908739
- Grant/Contract Number:
- AC02-76SF00515; CBET-1729397; CCF-1956054; N00014-21-1-4013; N00014-20-1-2084; W911NF-19-2-0026; EP/L015722/1
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Science Advances
- Additional Journal Information:
- Journal Volume: 8; Journal Issue: 20; Journal ID: ISSN 2375-2548
- Publisher:
- AAAS
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Wang, Xiao, Li, Shanshan, Jun, Hyungmin, John, Torsten, Zhang, Kaiming, Fowler, Hannah, Doye, Jonathan P.K., Chiu, Wah, and Bathe, Mark. Planar 2D wireframe DNA origami. United States: N. p., 2022.
Web. doi:10.1126/sciadv.abn0039.
Wang, Xiao, Li, Shanshan, Jun, Hyungmin, John, Torsten, Zhang, Kaiming, Fowler, Hannah, Doye, Jonathan P.K., Chiu, Wah, & Bathe, Mark. Planar 2D wireframe DNA origami. United States. https://doi.org/10.1126/sciadv.abn0039
Wang, Xiao, Li, Shanshan, Jun, Hyungmin, John, Torsten, Zhang, Kaiming, Fowler, Hannah, Doye, Jonathan P.K., Chiu, Wah, and Bathe, Mark. Fri .
"Planar 2D wireframe DNA origami". United States. https://doi.org/10.1126/sciadv.abn0039. https://www.osti.gov/servlets/purl/1908739.
@article{osti_1908739,
title = {Planar 2D wireframe DNA origami},
author = {Wang, Xiao and Li, Shanshan and Jun, Hyungmin and John, Torsten and Zhang, Kaiming and Fowler, Hannah and Doye, Jonathan P.K. and Chiu, Wah and Bathe, Mark},
abstractNote = {Two-dimensional (2D) DNA origami is widely used for applications ranging from excitonics to single-molecule biophysics. Conventional, single-layer 2D DNA origami exhibits flexibility and curvature in solution; however, that may limit its suitability as a 2D structural template. In contrast, 2D wireframe DNA origami rendered with six-helix bundle edges offers local control over duplex orientations with enhanced in-plane rigidity. Here, we investigate the 3D structure of these assemblies using cryo–electron microscopy (cryo-EM). 3D reconstructions reveal a high degree of planarity and homogeneity in solution for polygonal objects with and without internal mesh, enabling 10-Å resolution for a triangle. Coarse-grained simulations were in agreement with cryo-EM data, offering molecular structural insight into this class of 2D DNA origami. Our results suggest that these assemblies may be valuable for 2D material applications and geometries that require high structural fidelity together with local control over duplex orientations, rather than parallel duplex assembly.},
doi = {10.1126/sciadv.abn0039},
journal = {Science Advances},
number = 20,
volume = 8,
place = {United States},
year = {Fri May 20 00:00:00 EDT 2022},
month = {Fri May 20 00:00:00 EDT 2022}
}
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