Functional Characterization of NAC and MYB Transcription Factors Involved in Regulation of Biomass Production in Switchgrass (Panicum virgatum)
Abstract
Switchgrass is a promising biofuel feedstock due to its high biomass production and low agronomic input requirements. Because the bulk of switchgrass biomass used for biofuel production is lignocellulosic secondary walls, studies on secondary wall biosynthesis and its transcriptional regulation are imperative for designing strategies for genetic improvement of biomass production in switchgrass. Here, we report the identification and functional characterization of a group of switchgrass transcription factors, including several NACs (PvSWNs) and a MYB (PvMYB46A), for their involvement in regulating secondary wall biosynthesis. PvSWNs and PvMYB46A were found to be highly expressed in stems and their expression was closely associated with sclerenchyma cells. Overexpression of PvSWNs and PvMYB46A in Arabidopsis was shown to result in activation of the biosynthetic genes for cellulose, xylan and lignin and ectopic deposition of secondary walls in normally parenchymatous cells. Transactivation and complementation studies demonstrated that PvSWNs were able to activate the SNBE-driven GUS reporter gene and effectively rescue the secondary wall defects in the Arabidopsis snd1 nst1 double mutant, indicating that they are functional orthologs of Arabidopsis SWNs. Furthermore, we showed that PvMYB46A could activate the SMRE-driven GUS reporter gene and complement the Arabidopsis myb46 myb83 double mutant, suggesting that it is amore »
- Authors:
-
- University of Georgia, Athens, GA (United States)
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB); National Science Foundation (NSF)
- OSTI Identifier:
- 1904686
- Grant/Contract Number:
- FG02-03ER15415; ISO-1051900
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 8; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Arabidopsis thaliana; lignin; biosynthesis; cellulose; cell staining; leaves; reporter genes; transcription factors
Citation Formats
Zhong, Ruiqin, Yuan, Youxi, Spiekerman, John J., Guley, Joshua T., Egbosiuba, Janefrances C., and Ye, Zheng-Hua. Functional Characterization of NAC and MYB Transcription Factors Involved in Regulation of Biomass Production in Switchgrass (Panicum virgatum). United States: N. p., 2015.
Web. doi:10.1371/journal.pone.0134611.
Zhong, Ruiqin, Yuan, Youxi, Spiekerman, John J., Guley, Joshua T., Egbosiuba, Janefrances C., & Ye, Zheng-Hua. Functional Characterization of NAC and MYB Transcription Factors Involved in Regulation of Biomass Production in Switchgrass (Panicum virgatum). United States. https://doi.org/10.1371/journal.pone.0134611
Zhong, Ruiqin, Yuan, Youxi, Spiekerman, John J., Guley, Joshua T., Egbosiuba, Janefrances C., and Ye, Zheng-Hua. Thu .
"Functional Characterization of NAC and MYB Transcription Factors Involved in Regulation of Biomass Production in Switchgrass (Panicum virgatum)". United States. https://doi.org/10.1371/journal.pone.0134611. https://www.osti.gov/servlets/purl/1904686.
@article{osti_1904686,
title = {Functional Characterization of NAC and MYB Transcription Factors Involved in Regulation of Biomass Production in Switchgrass (Panicum virgatum)},
author = {Zhong, Ruiqin and Yuan, Youxi and Spiekerman, John J. and Guley, Joshua T. and Egbosiuba, Janefrances C. and Ye, Zheng-Hua},
abstractNote = {Switchgrass is a promising biofuel feedstock due to its high biomass production and low agronomic input requirements. Because the bulk of switchgrass biomass used for biofuel production is lignocellulosic secondary walls, studies on secondary wall biosynthesis and its transcriptional regulation are imperative for designing strategies for genetic improvement of biomass production in switchgrass. Here, we report the identification and functional characterization of a group of switchgrass transcription factors, including several NACs (PvSWNs) and a MYB (PvMYB46A), for their involvement in regulating secondary wall biosynthesis. PvSWNs and PvMYB46A were found to be highly expressed in stems and their expression was closely associated with sclerenchyma cells. Overexpression of PvSWNs and PvMYB46A in Arabidopsis was shown to result in activation of the biosynthetic genes for cellulose, xylan and lignin and ectopic deposition of secondary walls in normally parenchymatous cells. Transactivation and complementation studies demonstrated that PvSWNs were able to activate the SNBE-driven GUS reporter gene and effectively rescue the secondary wall defects in the Arabidopsis snd1 nst1 double mutant, indicating that they are functional orthologs of Arabidopsis SWNs. Furthermore, we showed that PvMYB46A could activate the SMRE-driven GUS reporter gene and complement the Arabidopsis myb46 myb83 double mutant, suggesting that it is a functional ortholog of Arabidopsis MYB46/MYB83. Together, these results indicate that PvSWNs and PvMYB46A are transcriptional switches involved in regulating secondary wall biosynthesis, which provides molecular tools for genetic manipulation of biomass production in switchgrass.},
doi = {10.1371/journal.pone.0134611},
journal = {PLoS ONE},
number = 8,
volume = 10,
place = {United States},
year = {Thu Aug 06 00:00:00 EDT 2015},
month = {Thu Aug 06 00:00:00 EDT 2015}
}
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The Biology and Agronomy of Switchgrass for Biofuels
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Involvement of Pinus taeda MYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis
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Identification of genes involved in cell wall biogenesis in grasses by differential gene expression profiling of elongating and non-elongating maize internodes
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The Poplar Glycosyltransferase GT47C is Functionally Conserved with
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The Poplar MYB Transcription Factors, PtrMYB3 and PtrMYB20, are Involved in the Regulation of Secondary Wall Biosynthesis
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Transcriptional Activation of Secondary Wall Biosynthesis by Rice and Maize NAC and MYB Transcription Factors
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- Plant and Cell Physiology, Vol. 52, Issue 10
Identification of Transcription Factors Involved in Rice Secondary Cell Wall Formation
journal, October 2013
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- Plant and Cell Physiology, Vol. 54, Issue 11
Transcription switches for protoxylem and metaxylem vessel formation
journal, August 2005
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Signal Transduction in Maize and Arabidopsis Mesophyll Protoplasts
journal, December 2001
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Mechanisms of Derived Unitegmy among Impatiens Species
journal, April 2005
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- The Plant Cell, Vol. 17, Issue 6
The NAC Transcription Factors NST1 and NST2 of Arabidopsis Regulate Secondary Wall Thickenings and Are Required for Anther Dehiscence
journal, October 2005
- Mitsuda, Nobutaka; Seki, Motoaki; Shinozaki, Kazuo
- The Plant Cell, Vol. 17, Issue 11
SND1, a NAC Domain Transcription Factor, Is a Key Regulator of Secondary Wall Synthesis in Fibers of Arabidopsis
journal, November 2006
- Zhong, Ruiqin; Demura, Taku; Ye, Zheng-Hua
- The Plant Cell, Vol. 18, Issue 11
The MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis
journal, September 2007
- Zhong, R.; Richardson, E. A.; Ye, Z.-H.
- The Plant Cell Online, Vol. 19, Issue 9, p. 2776-2792
A Battery of Transcription Factors Involved in the Regulation of Secondary Cell Wall Biosynthesis in Arabidopsis
journal, October 2008
- Zhong, R.; Lee, C.; Zhou, J.
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MYB58 and MYB63 Are Transcriptional Activators of the Lignin Biosynthetic Pathway during Secondary Cell Wall Formation in Arabidopsis
journal, January 2009
- Zhou, Jianli; Lee, Chanhui; Zhong, Ruiqin
- The Plant Cell, Vol. 21, Issue 1
Disruption of interfascicular fiber differentiation in an Arabidopsis mutant.
journal, December 1997
- Zhong, R.; Taylor, J. J.; Ye, Z. H.
- The Plant Cell, Vol. 9, Issue 12
Silencing of 4-coumarate:coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production
journal, July 2011
- Xu, Bin; Escamilla-Treviño, Luis L.; Sathitsuksanoh, Noppadon
- New Phytologist, Vol. 192, Issue 3
Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks
journal, October 2011
- Shen, Hui; He, Xianzhi; Poovaiah, Charleson R.
- New Phytologist, Vol. 193, Issue 1, p. 121-136
Comparative genomic analysis of the R2R3 MYB secondary cell wall regulators of Arabidopsis, poplar, rice, maize, and switchgrass
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- Zhao, Kangmei; Bartley, Laura E.
- BMC Plant Biology, Vol. 14, Issue 1
Advances in biotechnology and genomics of switchgrass
journal, January 2013
- Nageswara-Rao, Madhugiri; Soneji, Jaya R.; Kwit, Charles
- Biotechnology for Biofuels, Vol. 6, Issue 1
Monoclonal Antibodies to Plant Cell Wall Xylans and Arabinoxylans
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The Poplar MYB Master Switches Bind to the SMRE Site and Activate the Secondary Wall Biosynthetic Program during Wood Formation
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- PLoS ONE, Vol. 8, Issue 7
The Use of an Optical Brightener in the Study of Plant Structure
journal, January 1975
- Hughes, J.; McCully, Margaret E.
- Stain Technology, Vol. 50, Issue 5
Engineering the Oryza sativa cell wall with rice NAC transcription factors regulating secondary wall formation
journal, January 2013
- Yoshida, Kouki; Sakamoto, Shingo; Kawai, Tetsushi
- Frontiers in Plant Science, Vol. 4
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