Natural deep eutectic solvent mediated extrusion for continuous high-solid pretreatment of lignocellulosic biomass
Abstract
Several deep eutectic solvents (DESs) have been demonstrated to be highly effective for lignocellulosic biomass pretreatment, combining the advantages of simple synthesis, relatively low chemical cost and better biocompatibility. However, low biomass loading that is usually involved with DES pretreatment hinders its practical use. Here, a twin-screw extruder was used for pretreating biomass sorghum bagasse at solid loadings up to 50%, mediated by a neutral-pH DES, choline chloride : glycerol (ChCl : Gly). This continuous extrusion process led to high glucose and xylose yields of >85% from enzymatic saccharification of the pretreated sorghum. A combination of microscopic, spectroscopic, and X-ray diffraction analyses demonstrate a high degree of defibration and disruption of the biomass cell wall structures; however, little or no change in chemical compositions. Further results from gel permeation chromatographic (GPC) and nuclear magnetic resonance (NMR) spectroscopic analyses indicate that ChCl : Gly-mediated extrusion preserved the basic lignin structural characteristics with no significant differences between extruded biomass at a solid loading of 30% and 50%. This study demonstrates the potential of DES-mediated extrusion as a highly effective continuous high-solid biomass pretreatment technology for industrially relevant applications.
- Authors:
-
- Chinese Academy of Tropical Agricultural Sciences, Haikou (China). Haikou Experimental Station; Univ. of Kentucky, Lexington, KY (United States). Biosystems and Agricultural Engineering
- Univ. of Kentucky, Lexington, KY (United States). Biosystems and Agricultural Engineering
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JICS), Center for BioEnergy Innovation, Biosciences Division
- Chinese Academy of Tropical Agricultural Sciences, Haikou (China). Haikou Experimental Station
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon and Dept. of Chemical and Biomolecular Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JICS), Center for BioEnergy Innovation, Biosciences Division
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); USDA; National Natural Science Foundation of China (NSFC); Chinese Scholarship Council; Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences
- OSTI Identifier:
- 1651282
- Alternate Identifier(s):
- OSTI ID: 1642252
- Grant/Contract Number:
- AC05-00OR22725; 1015068; 1018315; 51708548; 201803260012; 17CXTD-05
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Green Chemistry
- Additional Journal Information:
- Journal Volume: 22; Journal Issue: 16; Journal ID: ISSN 1463-9262
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Ai, Binling, Li, Wenqi, Woomer, Joseph, Li, Mi, Pu, Yunqiao, Sheng, Zhanwu, Zheng, Lili, Adedeji, Akinbode, Ragauskas, Arthur J., and Shi, Jian. Natural deep eutectic solvent mediated extrusion for continuous high-solid pretreatment of lignocellulosic biomass. United States: N. p., 2020.
Web. doi:10.1039/d0gc01560a.
Ai, Binling, Li, Wenqi, Woomer, Joseph, Li, Mi, Pu, Yunqiao, Sheng, Zhanwu, Zheng, Lili, Adedeji, Akinbode, Ragauskas, Arthur J., & Shi, Jian. Natural deep eutectic solvent mediated extrusion for continuous high-solid pretreatment of lignocellulosic biomass. United States. https://doi.org/10.1039/d0gc01560a
Ai, Binling, Li, Wenqi, Woomer, Joseph, Li, Mi, Pu, Yunqiao, Sheng, Zhanwu, Zheng, Lili, Adedeji, Akinbode, Ragauskas, Arthur J., and Shi, Jian. Tue .
"Natural deep eutectic solvent mediated extrusion for continuous high-solid pretreatment of lignocellulosic biomass". United States. https://doi.org/10.1039/d0gc01560a. https://www.osti.gov/servlets/purl/1651282.
@article{osti_1651282,
title = {Natural deep eutectic solvent mediated extrusion for continuous high-solid pretreatment of lignocellulosic biomass},
author = {Ai, Binling and Li, Wenqi and Woomer, Joseph and Li, Mi and Pu, Yunqiao and Sheng, Zhanwu and Zheng, Lili and Adedeji, Akinbode and Ragauskas, Arthur J. and Shi, Jian},
abstractNote = {Several deep eutectic solvents (DESs) have been demonstrated to be highly effective for lignocellulosic biomass pretreatment, combining the advantages of simple synthesis, relatively low chemical cost and better biocompatibility. However, low biomass loading that is usually involved with DES pretreatment hinders its practical use. Here, a twin-screw extruder was used for pretreating biomass sorghum bagasse at solid loadings up to 50%, mediated by a neutral-pH DES, choline chloride : glycerol (ChCl : Gly). This continuous extrusion process led to high glucose and xylose yields of >85% from enzymatic saccharification of the pretreated sorghum. A combination of microscopic, spectroscopic, and X-ray diffraction analyses demonstrate a high degree of defibration and disruption of the biomass cell wall structures; however, little or no change in chemical compositions. Further results from gel permeation chromatographic (GPC) and nuclear magnetic resonance (NMR) spectroscopic analyses indicate that ChCl : Gly-mediated extrusion preserved the basic lignin structural characteristics with no significant differences between extruded biomass at a solid loading of 30% and 50%. This study demonstrates the potential of DES-mediated extrusion as a highly effective continuous high-solid biomass pretreatment technology for industrially relevant applications.},
doi = {10.1039/d0gc01560a},
journal = {Green Chemistry},
number = 16,
volume = 22,
place = {United States},
year = {Tue Jul 07 00:00:00 EDT 2020},
month = {Tue Jul 07 00:00:00 EDT 2020}
}
Works referenced in this record:
Deep eutectic solvent pretreatment enabling full utilization of switchgrass
journal, September 2018
- Chen, Zhu; Reznicek, Wesley D.; Wan, Caixia
- Bioresource Technology, Vol. 263
Facile pretreatment of lignocellulosic biomass using deep eutectic solvents
journal, November 2016
- Zhang, Cheng-Wu; Xia, Shu-Qian; Ma, Pei-Sheng
- Bioresource Technology, Vol. 219
Continuous pretreatment of sugarcane biomass using a twin-screw extruder
journal, March 2017
- Kuster Moro, Mariana; Sposina Sobral Teixeira, Ricardo; Sant’Ana da Silva, Ayla
- Industrial Crops and Products, Vol. 97
Novel dihydrogen-bonding deep eutectic solvents: Pretreatment of rice straw for butanol fermentation featuring enzyme recycling and high solvent yield
journal, February 2018
- Xing, Wanru; Xu, Guochao; Dong, Jinjun
- Chemical Engineering Journal, Vol. 333
Biocompatible Deep Eutectic Solvents Based on Choline Chloride: Characterization and Application to the Extraction of Rutin from Sophora japonica
journal, October 2015
- Zhao, Bing-Yi; Xu, Pei; Yang, Fu-Xi
- ACS Sustainable Chemistry & Engineering, Vol. 3, Issue 11
Xylooligomers are strong inhibitors of cellulose hydrolysis by enzymes
journal, December 2010
- Qing, Qing; Yang, Bin; Wyman, Charles E.
- Bioresource Technology, Vol. 101, Issue 24, p. 9624-9630
Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects
journal, January 2016
- Jönsson, Leif J.; Martín, Carlos
- Bioresource Technology, Vol. 199
Multiple hydrogen bond coordination in three-constituent deep eutectic solvents enhances lignin fractionation from biomass
journal, January 2018
- Xia, Qinqin; Liu, Yongzhuang; Meng, Juan
- Green Chemistry, Vol. 20, Issue 12
Techno-economic analysis of a lignocellulosic ethanol biorefinery with ionic liquid pre-treatment
journal, June 2011
- Klein-Marcuschamer, Daniel; Simmons, Blake A.; Blanch, Harvey W.
- Biofuels, Bioproducts and Biorefining, Vol. 5, Issue 5
Pretreatment with lower feed moisture and lower extrusion temperatures aids in the increase in the fermentable sugar yields from fine-milled Douglas-fir
journal, December 2018
- Gu, Bon-Jae; Wolcott, Michael P.; Ganjyal, Girish M.
- Bioresource Technology, Vol. 269
Assessing the Facile Pretreatments of Bagasse for Efficient Enzymatic Conversion and Their Impacts on Structural and Chemical Properties
journal, November 2018
- Satlewal, Alok; Agrawal, Ruchi; Das, Parthapratim
- ACS Sustainable Chemistry & Engineering, Vol. 7, Issue 1
Facile fractionation of lignocelluloses by biomass-derived deep eutectic solvent (DES) pretreatment for cellulose enzymatic hydrolysis and lignin valorization
journal, January 2019
- Shen, Xiao-Jun; Wen, Jia-Long; Mei, Qing-Qing
- Green Chemistry, Vol. 21, Issue 2
Design of low-cost ionic liquids for lignocellulosic biomass pretreatment
journal, January 2015
- George, Anthe; Brandt, Agnieszka; Tran, Kim
- Green Chemistry, Vol. 17, Issue 3
Isolation of Cellulolytic Enzyme Lignin from Wood Preswollen/Dissolved in Dimethyl Sulfoxide/ N -Methylimidazole
journal, March 2010
- Zhang, Aiping; Lu, Fachuang; Sun, Run-Cang
- Journal of Agricultural and Food Chemistry, Vol. 58, Issue 6
Physico-Chemical Conversion of Lignocellulose: Inhibitor Effects and Detoxification Strategies: A Mini Review
journal, February 2018
- Kim, Daehwan
- Molecules, Vol. 23, Issue 2
Towards an environmentally and economically sustainable biorefinery: heavy metal contaminated waste wood as a low-cost feedstock in a low-cost ionic liquid process
journal, January 2020
- Gschwend, Florence J. V.; Hennequin, Louis M.; Brandt-Talbot, Agnieszka
- Green Chemistry
Evaluation of toxicity and biodegradability of choline chloride based deep eutectic solvents
journal, February 2015
- Radošević, Kristina; Cvjetko Bubalo, Marina; Gaurina Srček, Višnje
- Ecotoxicology and Environmental Safety, Vol. 112
Biocompatible Choline-Based Deep Eutectic Solvents Enable One-Pot Production of Cellulosic Ethanol
journal, May 2018
- Xu, Feng; Sun, Jian; Wehrs, Maren
- ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 7
Physical and chemical characterizations of corn stover and poplar solids resulting from leading pretreatment technologies
journal, September 2009
- Kumar, Rajeev; Mago, Gaurav; Balan, Venkatesh
- Bioresource Technology, Vol. 100, Issue 17
Influence of furfural on anaerobic glycolytic kinetics ofSaccharomyces cerevisiae in batch culture
journal, February 1999
- Palmqvist, Eva; Almeida, Jonas S.; Hahn-H�gerdal, B�rbel
- Biotechnology and Bioengineering, Vol. 62, Issue 4
Pretreatment of wheat straw leads to structural changes and improved enzymatic hydrolysis
journal, January 2018
- Zheng, Qi; Zhou, Tiantian; Wang, Yibin
- Scientific Reports, Vol. 8, Issue 1
Application of cellulase and hemicellulase to pure xylan, pure cellulose, and switchgrass solids from leading pretreatments
journal, December 2011
- Shi, Jian; Ebrik, Mirvat A.; Yang, Bin
- Bioresource Technology, Vol. 102, Issue 24
Enzymatic hydrolysis of corn crop residues with high solid loadings: New insights into the impact of bioextrusion on biomass deconstruction using carbohydrate-binding modules
journal, June 2019
- Gatt, Etienne; Khatri, Vinay; Bley, Julien
- Bioresource Technology, Vol. 282
Techno‐economic evaluation of a natural deep eutectic solvent‐based biorefinery: Exploring different design scenarios
journal, May 2020
- Kumar, Adepu K.; Sharma, Shaishav; Dixit, Gaurav
- Biofuels, Bioproducts and Biorefining, Vol. 14, Issue 4
Dry jet-wet spinning of strong cellulose filaments from ionic liquid solution
journal, September 2014
- Hauru, Lauri K. J.; Hummel, Michael; Michud, Anne
- Cellulose, Vol. 21, Issue 6
Economic impact of total solids loading on enzymatic hydrolysis of dilute acid pretreated corn stover
journal, April 2010
- Humbird, David; Mohagheghi, Ali; Dowe, Nancy
- Biotechnology Progress, Vol. 26, Issue 5
Deep eutectic solvents’ ability to solubilize lignin, cellulose, and hemicellulose; thermal stability; and density
journal, August 2017
- Lynam, Joan G.; Kumar, Narendra; Wong, Mark J.
- Bioresource Technology, Vol. 238
Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover
journal, June 2011
- Qing, Qing; Wyman, Charles E.
- Biotechnology for Biofuels, Vol. 4, Issue 1, Article No: 18
Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass
journal, April 2011
- Kim, Youngmi; Ximenes, Eduardo; Mosier, Nathan S.
- Enzyme and Microbial Technology, Vol. 48, Issue 4-5
Effect of constituents molar ratios of deep eutectic solvents on rice straw fractionation efficiency and the micro-mechanism investigation
journal, September 2018
- Hou, Xue-Dan; Lin, Kai-Peng; Li, Ao-Lin
- Industrial Crops and Products, Vol. 120
New insights into the structure and composition of technical lignins: a comparative characterisation study
journal, January 2016
- Constant, Sandra; Wienk, Hans L. J.; Frissen, Augustinus E.
- Green Chemistry, Vol. 18, Issue 9
The path forward for lignocellulose biorefineries: Bottlenecks, solutions, and perspective on commercialization
journal, September 2018
- Chandel, Anuj Kumar; Garlapati, Vijay Kumar; Singh, Akhilesh Kumar
- Bioresource Technology, Vol. 264
Characterization and Catalytic Transfer Hydrogenolysis of Deep Eutectic Solvent Extracted Sorghum Lignin to Phenolic Compounds
journal, June 2018
- Das, Lalitendu; Li, Mi; Stevens, Joseph
- ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 8
Waste glycerol from biodiesel synthesis as a component in deep eutectic solvents
journal, October 2015
- Bewley, Blake R.; Berkaliev, Asem; Henriksen, Hanne
- Fuel Processing Technology, Vol. 138
IONCELL-P&F: Pulp Fractionation and Fiber Spinning with Ionic Liquids
journal, July 2016
- Stepan, Agnes M.; Michud, Anne; Hellstén, Sanna
- Industrial & Engineering Chemistry Research, Vol. 55, Issue 29
Fractionation and characterization of lignin streams from unique high-lignin content endocarp feedstocks
journal, November 2018
- Li, Wenqi; Amos, Kirtley; Li, Mi
- Biotechnology for Biofuels, Vol. 11, Issue 1
Continuous pretreatment of sugarcane bagasse at high loading in an ionic liquid using a twin-screw extruder
journal, January 2013
- da Silva, Ayla Sant'Ana; Teixeira, Ricardo Sposina Sobral; Endo, Takashi
- Green Chemistry, Vol. 15, Issue 7
pK a -Values of Guaiacyl and Syringyl Phenols Related to Lignin
journal, August 2000
- Ragnar, Martin; Lindgren, Christofer T.; Nilvebrant, Nils-Olof
- Journal of Wood Chemistry and Technology, Vol. 20, Issue 3
An economically viable ionic liquid for the fractionation of lignocellulosic biomass
journal, January 2017
- Brandt-Talbot, Agnieszka; Gschwend, Florence J. V.; Fennell, Paul S.
- Green Chemistry, Vol. 19, Issue 13
Linking lignin source with structural and electrochemical properties of lignin-derived carbon materials
journal, January 2018
- Li, Wenqi; Zhang, Yan; Das, Lalitendu
- RSC Advances, Vol. 8, Issue 68
Sugar production from wheat straw biomass by alkaline extrusion and enzymatic hydrolysis
journal, February 2016
- Coimbra, Michelle Cardoso; Duque, Aleta; Saéz, Felicia
- Renewable Energy, Vol. 86
Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates
journal, June 2014
- Meng, Xianzhi; Ragauskas, Arthur Jonas
- Current Opinion in Biotechnology, Vol. 27
Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance
journal, January 2010
- Park, Sunkyu; Baker, John O.; Himmel, Michael E.
- Biotechnology for Biofuels, Vol. 3, Issue 1
Advances in lignin valorization towards bio-based chemicals and fuels: Lignin biorefinery
journal, November 2019
- Cao, Yang; Chen, Season S.; Zhang, Shicheng
- Bioresource Technology, Vol. 291
Extrusion as a pretreatment for lignocellulosic biomass: Fundamentals and applications
journal, December 2017
- Duque, Aleta; Manzanares, Paloma; Ballesteros, Mercedes
- Renewable Energy, Vol. 114
Natural Deep Eutectic Solvents: Properties, Applications, and Perspectives
journal, November 2017
- Liu, Yang; Friesen, J. Brent; McAlpine, James B.
- Journal of Natural Products, Vol. 81, Issue 3
High-Solid Lignocellulose Processing Enabled by Natural Deep Eutectic Solvent for Lignin Extraction and Industrially Relevant Production of Renewable Chemicals
journal, July 2018
- Chen, Zhu; Bai, Xianglan; A., Lusi
- ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 9
Optimization of integrated alkaline–extrusion pretreatment of barley straw for sugar production by enzymatic hydrolysis
journal, May 2013
- Duque, A.; Manzanares, P.; Ballesteros, I.
- Process Biochemistry, Vol. 48, Issue 5-6
Understanding Factors that Limit Enzymatic Hydrolysis of Biomass: Characterization of Pretreated Corn Stover
journal, January 2005
- Laureano-Perez, Lizbeth; Teymouri, Farzaneh; Alizadeh, Hasan
- Applied Biochemistry and Biotechnology, Vol. 124, Issue 1-3
Effect of crystallinity and degree of polymerization of cellulose on enzymatic saccharification
journal, October 1984
- Puri, Vinod P.
- Biotechnology and Bioengineering, Vol. 26, Issue 10
Enzymatic saccharification of woody biomass micro/nanofibrillated by continuous extrusion process I – Effect of additives with cellulose affinity
journal, January 2009
- Lee, Seung-Hwan; Teramoto, Yoshikuni; Endo, Takashi
- Bioresource Technology, Vol. 100, Issue 1
Natural Deep Eutectic Solvents – Solvents for the 21st Century
journal, March 2014
- Paiva, Alexandre; Craveiro, Rita; Aroso, Ivo
- ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 5
New natural and renewable low transition temperature mixtures (LTTMs): screening as solvents for lignocellulosic biomass processing
journal, January 2012
- Francisco, María; van den Bruinhorst, Adriaan; Kroon, Maaike C.
- Green Chemistry, Vol. 14, Issue 8
Contribution of Deep Eutectic Solvents for Biomass Processing: Opportunities, Challenges, and Limitations
journal, March 2015
- Vigier, Karine De Oliveira; Chatel, Gregory; Jérôme, François
- ChemCatChem, Vol. 7, Issue 8
One-pot ionic liquid pretreatment and saccharification of switchgrass
journal, January 2013
- Shi, Jian; Gladden, John M.; Sathitsuksanoh, Noppadon
- Green Chemistry, Vol. 15, Issue 9
Glycerol from biodiesel production: Technological paths for sustainability
journal, May 2018
- Monteiro, Marcos Roberto; Kugelmeier, Cristie Luis; Pinheiro, Rafael Sanaiotte
- Renewable and Sustainable Energy Reviews, Vol. 88