Phosphoric acid based pretreatment of switchgrass and fermentation of entire slurry to ethanol using a simplified process
Abstract
Switchgrass (Alamo) was pretreated with phosphoric acid (0.75 and 1%, w/w) at three temperatures (160, 175 and 190 °C) and time (5, 7.5 and 10 min) using a steam gun. The slurry after pretreatment was liquefied by enzymes and the released sugars were fermented in a simultaneous saccharification and co-fermentation process to ethanol using ethanologenic Escherichia coli strain SL100. Among the three variables in pretreatment, temperature and time were critical in supporting ethanol titer and yield. Enzyme hydrolysis significantly increased the concentration of furans in slurries, apparently due to release of furans bound to the solids. The highest ethanol titer of 21.2±0.3 g/L ethanol obtained at the pretreatment condition of 190-1-7.5 (temperature-acid concentration-time) and 10% solids loading accounted for 190±2.9 g ethanol /kg of raw switch grass. Furthermore, this converts to 61.7 gallons of ethanol per ton of dry switchgrass, a value that is comparable to other published pretreatment conditions.
- Authors:
-
- Dept. of Microbiology and Cell Science, Gainesville, FL (United States); Dept. of Agricultural and Biological Engineering, Gainesville, FL (United States)
- Dept. of Microbiology and Cell Science, Gainesville, FL (United States); Univ. of Florida, Gainesville, FL (United States)
- Univ. of Florida, Gainesville, FL (United States)
- Dept. of Agricultural and Biological Engineering, Gainesville, FL (United States)
- Dept. of Microbiology and Cell Science, Gainesville, FL (United States)
- Publication Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE Office of International Affairs (IA); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
- OSTI Identifier:
- 1766312
- Alternate Identifier(s):
- OSTI ID: 1496494; OSTI ID: 1661472
- Grant/Contract Number:
- PI0000031
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Bioresource Technology
- Additional Journal Information:
- Journal Volume: 251; Journal Issue: 251; Journal ID: ISSN 0960-8524
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Switchgrass; Fermentation; phosphoric acid pretreatment; ethanol; L+SScF; switchgrass; fermentation; L + SScF
Citation Formats
Wu, Wei, Rondon, Vanessa, Weeks, Kalvin, Pullammanappallil, Pratap, Ingram, Lonnie O., and Shanmugam, K. T. Phosphoric acid based pretreatment of switchgrass and fermentation of entire slurry to ethanol using a simplified process. United States: N. p., 2017.
Web. doi:10.1016/j.biortech.2017.12.041.
Wu, Wei, Rondon, Vanessa, Weeks, Kalvin, Pullammanappallil, Pratap, Ingram, Lonnie O., & Shanmugam, K. T. Phosphoric acid based pretreatment of switchgrass and fermentation of entire slurry to ethanol using a simplified process. United States. https://doi.org/10.1016/j.biortech.2017.12.041
Wu, Wei, Rondon, Vanessa, Weeks, Kalvin, Pullammanappallil, Pratap, Ingram, Lonnie O., and Shanmugam, K. T. Sat .
"Phosphoric acid based pretreatment of switchgrass and fermentation of entire slurry to ethanol using a simplified process". United States. https://doi.org/10.1016/j.biortech.2017.12.041. https://www.osti.gov/servlets/purl/1766312.
@article{osti_1766312,
title = {Phosphoric acid based pretreatment of switchgrass and fermentation of entire slurry to ethanol using a simplified process},
author = {Wu, Wei and Rondon, Vanessa and Weeks, Kalvin and Pullammanappallil, Pratap and Ingram, Lonnie O. and Shanmugam, K. T.},
abstractNote = {Switchgrass (Alamo) was pretreated with phosphoric acid (0.75 and 1%, w/w) at three temperatures (160, 175 and 190 °C) and time (5, 7.5 and 10 min) using a steam gun. The slurry after pretreatment was liquefied by enzymes and the released sugars were fermented in a simultaneous saccharification and co-fermentation process to ethanol using ethanologenic Escherichia coli strain SL100. Among the three variables in pretreatment, temperature and time were critical in supporting ethanol titer and yield. Enzyme hydrolysis significantly increased the concentration of furans in slurries, apparently due to release of furans bound to the solids. The highest ethanol titer of 21.2±0.3 g/L ethanol obtained at the pretreatment condition of 190-1-7.5 (temperature-acid concentration-time) and 10% solids loading accounted for 190±2.9 g ethanol /kg of raw switch grass. Furthermore, this converts to 61.7 gallons of ethanol per ton of dry switchgrass, a value that is comparable to other published pretreatment conditions.},
doi = {10.1016/j.biortech.2017.12.041},
journal = {Bioresource Technology},
number = 251,
volume = 251,
place = {United States},
year = {Sat Dec 16 00:00:00 EST 2017},
month = {Sat Dec 16 00:00:00 EST 2017}
}
Web of Science
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