Simultaneous fermentation of biomass-derived sugars to ethanol by a co-culture of an engineered Escherichia coli and Saccharomyces cerevisiae
Abstract
Microorganisms ferment xylose at high rate only when glucose concentration in the medium falls below a critical level. Since the specific productivity of product is highest during exponential to early stationary phase of growth, a glucose utilization negative ethanologenic E. coli (strain LW419a) was constructed for high rate of xylose fermentation in combination with Turbo yeast. This co-culture fermented all the released sugars in an acid/enzyme-treated sugar cane bagasse slurry (10% solids) to an ethanol titer of 24.9 ± 0.8 g.L-1 (70 % of the theoretical yield) in <30 h. Ethanol titer increased to 48.6 ± 1.04 g.L-1 (yield, 0.45 g.g-1 sugars) at a solids content of 20% and the highest rate of xylose consumption was 1.58 ± 0.21 g.L–1.h–1. Furthermore, this study demonstrates the potential of a co-culture of strain LW419a and yeast to rapidly ferment all the sugars in pretreated biomass slurries to ethanol at their respective highest rates.
- Authors:
-
- Univ. of Florida, Gainesville, FL (United States)
- Publication Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE Office of International Affairs (IA)
- OSTI Identifier:
- 1766299
- Alternate Identifier(s):
- OSTI ID: 1636704
- Grant/Contract Number:
- PI0000031
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Bioresource Technology
- Additional Journal Information:
- Journal Volume: 273; Journal Issue: 273; Journal ID: ISSN 0960-8524
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; co-fermentation; ethanol, E. coli; biomass; xylose
Citation Formats
Wang, Liang, York, Sean W., Ingram, Lonnie O., and Shanmugam, K. T. Simultaneous fermentation of biomass-derived sugars to ethanol by a co-culture of an engineered Escherichia coli and Saccharomyces cerevisiae. United States: N. p., 2018.
Web. doi:10.1016/j.biortech.2018.11.016.
Wang, Liang, York, Sean W., Ingram, Lonnie O., & Shanmugam, K. T. Simultaneous fermentation of biomass-derived sugars to ethanol by a co-culture of an engineered Escherichia coli and Saccharomyces cerevisiae. United States. https://doi.org/10.1016/j.biortech.2018.11.016
Wang, Liang, York, Sean W., Ingram, Lonnie O., and Shanmugam, K. T. Wed .
"Simultaneous fermentation of biomass-derived sugars to ethanol by a co-culture of an engineered Escherichia coli and Saccharomyces cerevisiae". United States. https://doi.org/10.1016/j.biortech.2018.11.016. https://www.osti.gov/servlets/purl/1766299.
@article{osti_1766299,
title = {Simultaneous fermentation of biomass-derived sugars to ethanol by a co-culture of an engineered Escherichia coli and Saccharomyces cerevisiae},
author = {Wang, Liang and York, Sean W. and Ingram, Lonnie O. and Shanmugam, K. T.},
abstractNote = {Microorganisms ferment xylose at high rate only when glucose concentration in the medium falls below a critical level. Since the specific productivity of product is highest during exponential to early stationary phase of growth, a glucose utilization negative ethanologenic E. coli (strain LW419a) was constructed for high rate of xylose fermentation in combination with Turbo yeast. This co-culture fermented all the released sugars in an acid/enzyme-treated sugar cane bagasse slurry (10% solids) to an ethanol titer of 24.9 ± 0.8 g.L-1 (70 % of the theoretical yield) in <30 h. Ethanol titer increased to 48.6 ± 1.04 g.L-1 (yield, 0.45 g.g-1 sugars) at a solids content of 20% and the highest rate of xylose consumption was 1.58 ± 0.21 g.L–1.h–1. Furthermore, this study demonstrates the potential of a co-culture of strain LW419a and yeast to rapidly ferment all the sugars in pretreated biomass slurries to ethanol at their respective highest rates.},
doi = {10.1016/j.biortech.2018.11.016},
journal = {Bioresource Technology},
number = 273,
volume = 273,
place = {United States},
year = {Wed Nov 07 00:00:00 EST 2018},
month = {Wed Nov 07 00:00:00 EST 2018}
}
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