Simultaneous fermentation of biomass-derived sugars to ethanol by a co-culture of an engineered Escherichia coli and Saccharomyces cerevisiae

Wang, Liang; York, Sean W.; Ingram, Lonnie O.; Shanmugam, K. T.

doi:10.1016/j.biortech.2018.11.016

Title: Simultaneous fermentation of biomass-derived sugars to ethanol by a co-culture of an engineered Escherichia coli and Saccharomyces cerevisiae

Journal Article · 06 November 2018 · Bioresource Technology

DOI: https://doi.org/10.1016/j.biortech.2018.11.016 · OSTI ID:1766299

Wang, Liang ^[1]; York, Sean W. ^[2]; Ingram, Lonnie O. ^[2]; Shanmugam, K. T. ^[2]

Univ. of Florida, Gainesville, FL (United States); University of Florida
Univ. of Florida, Gainesville, FL (United States)

Microorganisms ferment xylose at high rate only when glucose concentration in the medium falls below a critical level. Since the specific productivity of product is highest during exponential to early stationary phase of growth, a glucose utilization negative ethanologenic E. coli (strain LW419a) was constructed for high rate of xylose fermentation in combination with Turbo yeast. This co-culture fermented all the released sugars in an acid/enzyme-treated sugar cane bagasse slurry (10% solids) to an ethanol titer of 24.9 ± 0.8 g.L^-1 (70 % of the theoretical yield) in <30 h. Ethanol titer increased to 48.6 ± 1.04 g.L^-1 (yield, 0.45 g.g^-1 sugars) at a solids content of 20% and the highest rate of xylose consumption was 1.58 ± 0.21 g.L^–1.h^–1. Furthermore, this study demonstrates the potential of a co-culture of strain LW419a and yeast to rapidly ferment all the sugars in pretreated biomass slurries to ethanol at their respective highest rates.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE; USDOE Office of International Affairs (IA)

Grant/Contract Number:: PI0000031

OSTI ID:: 1766299

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: 273 Vol. 273; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Title: Simultaneous fermentation of biomass-derived sugars to ethanol by a co-culture of an engineered Escherichia coli and Saccharomyces cerevisiae

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