skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus

Abstract

Use of oleaginous microorganisms as “micro-factories” for accumulation of single cell oils for biofuel production has increased significantly to mitigate growing energy demands, resulting in efforts to upgrade industrial waste, such as second-generation lignocellulosic residues, into potential feedstocks. Dilute-acid pretreatment (DAP) is commonly used to alter the physicochemical properties of lignocellulosic materials and is typically coupled with simultaneous saccharification and fermentation (SSF) for conversion of sugars into ethanol. The resulting DAP residues are usually processed as a waste stream, e.g. burned for power, but this provides minimal value. Alternatively, these wastes can be utilized as feedstock to generate lipids, which can be converted to biofuel. DAP-SSF residues were generated from pine, poplar, and switchgrass. High performance liquid chromatography revealed less than 0.13% monomeric sugars in the dry residue. Fourier transform infrared spectroscopy was indicative of the presence of lignin and polysaccharides. Gel permeation chromatography suggested the bacterial strains preferred molecules with molecular weight ~ 400–500 g/mol. DAP-SSF residues were used as the sole carbon source for lipid production by Rhodococcus opacus DSM 1069 and PD630 in batch fermentations. Depending on the strain of Rhodococcus employed, 9–11 lipids for PD630 and DSM 1069 were observed, at a final concentration of ~more » 15 mg/L fatty acid methyl esters (FAME) detected. Though the DAP-SSF substrate resulted in low FAME titers, novel analysis of solid-state fermentations was investigated, which determined that DAP-SSF residues could be a viable feedstock for lipid generation.« less

Authors:
; ; ; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Texas A & M Univ., College Station, TX (United States); Univ. of Tennessee, Knoxville, TN (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1395608
Alternate Identifier(s):
OSTI ID: 1471890
Grant/Contract Number:  
EE0006112; AC05-00OR22725
Resource Type:
Published Article
Journal Name:
AMB Express
Additional Journal Information:
Journal Name: AMB Express Journal Volume: 7 Journal Issue: 1; Journal ID: ISSN 2191-0855
Publisher:
Springer
Country of Publication:
Germany
Language:
English
Subject:
09 BIOMASS FUELS; dilute-acid pretreatment; simultaneous saccharification and fermentation; Rhodococcus opacus; biofuel; lipids

Citation Formats

Le, Rosemary K., Das, Parthapratim, Mahan, Kristina M., Anderson, Seth A., Wells, Tyrone, Yuan, Joshua S., and Ragauskas, Arthur J. Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus. Germany: N. p., 2017. Web. doi:10.1186/s13568-017-0484-0.
Le, Rosemary K., Das, Parthapratim, Mahan, Kristina M., Anderson, Seth A., Wells, Tyrone, Yuan, Joshua S., & Ragauskas, Arthur J. Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus. Germany. doi:10.1186/s13568-017-0484-0.
Le, Rosemary K., Das, Parthapratim, Mahan, Kristina M., Anderson, Seth A., Wells, Tyrone, Yuan, Joshua S., and Ragauskas, Arthur J. Fri . "Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus". Germany. doi:10.1186/s13568-017-0484-0.
@article{osti_1395608,
title = {Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus},
author = {Le, Rosemary K. and Das, Parthapratim and Mahan, Kristina M. and Anderson, Seth A. and Wells, Tyrone and Yuan, Joshua S. and Ragauskas, Arthur J.},
abstractNote = {Use of oleaginous microorganisms as “micro-factories” for accumulation of single cell oils for biofuel production has increased significantly to mitigate growing energy demands, resulting in efforts to upgrade industrial waste, such as second-generation lignocellulosic residues, into potential feedstocks. Dilute-acid pretreatment (DAP) is commonly used to alter the physicochemical properties of lignocellulosic materials and is typically coupled with simultaneous saccharification and fermentation (SSF) for conversion of sugars into ethanol. The resulting DAP residues are usually processed as a waste stream, e.g. burned for power, but this provides minimal value. Alternatively, these wastes can be utilized as feedstock to generate lipids, which can be converted to biofuel. DAP-SSF residues were generated from pine, poplar, and switchgrass. High performance liquid chromatography revealed less than 0.13% monomeric sugars in the dry residue. Fourier transform infrared spectroscopy was indicative of the presence of lignin and polysaccharides. Gel permeation chromatography suggested the bacterial strains preferred molecules with molecular weight ~ 400–500 g/mol. DAP-SSF residues were used as the sole carbon source for lipid production by Rhodococcus opacus DSM 1069 and PD630 in batch fermentations. Depending on the strain of Rhodococcus employed, 9–11 lipids for PD630 and DSM 1069 were observed, at a final concentration of ~ 15 mg/L fatty acid methyl esters (FAME) detected. Though the DAP-SSF substrate resulted in low FAME titers, novel analysis of solid-state fermentations was investigated, which determined that DAP-SSF residues could be a viable feedstock for lipid generation.},
doi = {10.1186/s13568-017-0484-0},
journal = {AMB Express},
number = 1,
volume = 7,
place = {Germany},
year = {2017},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1186/s13568-017-0484-0

Citation Metrics:
Cited by: 5 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014

  • Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
  • Science, Vol. 344, Issue 6185, p. 1246843-1246843
  • DOI: 10.1126/science.1246843

Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid
journal, May 2011


Accumulation and mobilization of storage lipids by Rhodococcus opacus PD630 and Rhodococcus ruber NCIMB 40126
journal, August 2000

  • Alvarez, H. M.; Kalscheuer, R.; Steinbüchel, A.
  • Applied Microbiology and Biotechnology, Vol. 54, Issue 2
  • DOI: 10.1007/s002530000395

Lipids from heterotrophic microbes: advances in metabolism research
journal, February 2011


From sugars to biodiesel using microalgae and yeast
journal, January 2016

  • Gomez, Jose A.; Höffner, Kai; Barton, Paul I.
  • Green Chemistry, Vol. 18, Issue 2
  • DOI: 10.1039/C5GC01843A

Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci
journal, January 2017

  • Le, Rosemary K.; Wells Jr., Tyrone; Das, Parthapratim
  • RSC Advances, Vol. 7, Issue 7
  • DOI: 10.1039/C6RA28033A

Physicochemical Structural Changes of Poplar and Switchgrass during Biomass Pretreatment and Enzymatic Hydrolysis
journal, July 2016


Switchgrass as an energy crop for biofuel production: A review of its ligno-cellulosic chemical properties
journal, January 2010

  • David, Kasi; Ragauskas, Arthur J.
  • Energy & Environmental Science, Vol. 3, Issue 9
  • DOI: 10.1039/b926617h

Evaluation of oil-producing algae as potential biodiesel feedstock
journal, April 2013


Towards lignin consolidated bioprocessing: simultaneous lignin depolymerization and product generation by bacteria
journal, January 2015

  • Salvachúa, Davinia; Karp, Eric M.; Nimlos, Claire T.
  • Green Chemistry, Vol. 17, Issue 11
  • DOI: 10.1039/C5GC01165E

Comparison of bioethanol production of simultaneous saccharification & fermentation and separation hydrolysis & fermentation from cellulose-rich barley straw
journal, March 2012

  • Won, Kyung Yoen; Kim, Young Soo; Oh, Kyeong Keun
  • Korean Journal of Chemical Engineering, Vol. 29, Issue 10
  • DOI: 10.1007/s11814-012-0019-y

Bioconversion of lignocellulosic pretreatment effluent via oleaginous Rhodococcus opacus DSM 1069
journal, January 2015


Chemical and Physicochemical Pretreatment of Lignocellulosic Biomass: A Review
journal, January 2011

  • Brodeur, Gary; Yau, Elizabeth; Badal, Kimberly
  • Enzyme Research, Vol. 2011
  • DOI: 10.4061/2011/787532

Microbial utilization of lignin: available biotechnologies for its degradation and valorization
journal, August 2016

  • Palazzolo, Martín A.; Kurina-Sanz, Marcela
  • World Journal of Microbiology and Biotechnology, Vol. 32, Issue 10
  • DOI: 10.1007/s11274-016-2128-y

Triacylglycerols in prokaryotic microorganisms
journal, December 2002


Lignin Conversion: Opportunities and Challenges for the Integrated Biorefinery
journal, June 2016

  • Xie, Shangxian; Ragauskas, Arthur J.; Yuan, Joshua S.
  • Industrial Biotechnology, Vol. 12, Issue 3
  • DOI: 10.1089/ind.2016.0007

Biological valorization of low molecular weight lignin
journal, December 2016


Opportunities and challenges in biological lignin valorization
journal, December 2016


Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production
journal, April 2009

  • Kumar, Parveen; Barrett, Diane M.; Delwiche, Michael J.
  • Industrial & Engineering Chemistry Research, Vol. 48, Issue 8, p. 3713-3729
  • DOI: 10.1021/ie801542g

Pyrolysis Oil-Based Lipid Production as Biodiesel Feedstock by Rhodococcus opacus
journal, November 2014

  • Wei, Zhen; Zeng, Guangming; Kosa, Matyas
  • Applied Biochemistry and Biotechnology, Vol. 175, Issue 2
  • DOI: 10.1007/s12010-014-1305-4

A short review on SSF – an interesting process option for ethanol production from lignocellulosic feedstocks
journal, January 2008

  • Olofsson, Kim; Bertilsson, Magnus; Lidén, Gunnar
  • Biotechnology for Biofuels, Vol. 1, Issue 1
  • DOI: 10.1186/1754-6834-1-7

Adipic acid production from lignin
journal, January 2015

  • Vardon, Derek R.; Franden, Mary Ann; Johnson, Christopher W.
  • Energy & Environmental Science, Vol. 8, Issue 2
  • DOI: 10.1039/C4EE03230F

Production of renewable jet fuel range alkanes and commodity chemicals from integrated catalytic processing of biomass
journal, January 2014

  • Bond, Jesse Q.; Upadhye, Aniruddha A.; Olcay, Hakan
  • Energy Environ. Sci., Vol. 7, Issue 4
  • DOI: 10.1039/C3EE43846E

Biomass Characterization of Morphological Portions of Alamo Switchgrass
journal, July 2011

  • Hu, Zhoujian; Foston, Marcus B.; Ragauskas, Arthur J.
  • Journal of Agricultural and Food Chemistry, Vol. 59, Issue 14
  • DOI: 10.1021/jf104844r

Bioconversion of oxygen-pretreated Kraft lignin to microbial lipid with oleaginous Rhodococcus opacus DSM 1069
journal, January 2015

  • Wei, Zhen; Zeng, Guangming; Huang, Fang
  • Green Chemistry, Vol. 17, Issue 5
  • DOI: 10.1039/C5GC00422E

Biotechnological opportunities with the β-ketoadipate pathway
journal, December 2012


Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol
journal, December 2005


Simultaneous saccharification and fermentation of several lignocellulosic feedstocks to fuel ethanol
journal, January 1992


Modeling simultaneous saccharification and fermentation of lignocellulose to ethanol in batch and continuous reactors
journal, September 1995


The potential for agro-industrial waste utilization using oleaginous yeast for the production of biodiesel
journal, May 2014


Bioconversion of lignin model compounds with oleaginous Rhodococci
journal, December 2011


Microbial lipid production by oleaginous Rhodococci cultured in lignocellulosic autohydrolysates
journal, July 2015

  • Wei, Zhen; Zeng, Guangming; Huang, Fang
  • Applied Microbiology and Biotechnology, Vol. 99, Issue 17
  • DOI: 10.1007/s00253-015-6752-5

Qualitative and quantitative analysis of lignocellulosic biomass using infrared techniques: A mini-review
journal, April 2013


Lignin valorization through integrated biological funneling and chemical catalysis
journal, August 2014

  • Linger, J. G.; Vardon, D. R.; Guarnieri, M. T.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018
  • DOI: 10.1073/pnas.1410657111

Screening of Oleaginous Yeast Strains Tolerant to Lignocellulose Degradation Compounds
journal, January 2009

  • Chen, Xi; Li, Zihui; Zhang, Xiaoxi
  • Applied Biochemistry and Biotechnology, Vol. 159, Issue 3
  • DOI: 10.1007/s12010-008-8491-x

Simultaneous saccharification and fermentation of cellulose to lactic acid
journal, January 1991

  • Abe, Shin-ichiro; Takagi, Motoyoshi
  • Biotechnology and Bioengineering, Vol. 37, Issue 1
  • DOI: 10.1002/bit.260370113

Biodiesel from lignocellulosic biomass – Prospects and challenges
journal, November 2012


Sustainability of supply or the planet: a review of potential drop-in alternative aviation fuels
journal, January 2010

  • Rye, L.; Blakey, S.; Wilson, C. W.
  • Energy Environ. Sci., Vol. 3, Issue 1
  • DOI: 10.1039/B918197K

Lignin Pyrolysis Components and Upgrading—Technology Review
journal, February 2013


A review of cellulosic biofuel commercial-scale projects in the United States
journal, February 2013

  • Brown, Tristan R.; Brown, Robert C.
  • Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 3
  • DOI: 10.1002/bbb.1387

Lignin to lipid bioconversion by oleaginous Rhodococci
journal, January 2013

  • Kosa, Matyas; Ragauskas, Arthur J.
  • Green Chemistry, Vol. 15, Issue 8
  • DOI: 10.1039/c3gc40434j

    Works referencing / citing this record:

    Cooperative valorization of lignin and residual sugar to polyhydroxyalkanoate (PHA) for enhanced yield and carbon utilization in biorefineries
    journal, January 2019

    • Liu, Zhi-Hua; Shinde, Somnath; Xie, Shangxian
    • Sustainable Energy & Fuels, Vol. 3, Issue 8
    • DOI: 10.1039/c9se00021f

    Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains
    journal, March 2019