Glycoconjugates and methods
Abstract
Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.
- Inventors:
-
- Albany, CA
- Berkeley, CA
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- OSTI Identifier:
- 873035
- Patent Number(s):
- 6075134
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- glycoconjugates; methods; functionalized; contacting; cell; monosaccharide; incubating; conditions; whereby; internalizes; ii; biochemically; processes; saccharide; iii; conjugates; carrier; form; glycoconjugate; extracellularly; expresses; extracellular; comprising; selectively; reactive; functional; forming; products; comprise; expressed; agent; reacts; product; subject; compositions; cyto-compatible; monosaccharides; nitrogen; linked; surface; cells; saccharides; conditions whereby; cell surface; reactive functional; cells comprising; /999/424/
Citation Formats
Bertozzi, Carolyn, Yarema, Kevin J, and Mahal, Lara K. Glycoconjugates and methods. United States: N. p., 2000.
Web.
Bertozzi, Carolyn, Yarema, Kevin J, & Mahal, Lara K. Glycoconjugates and methods. United States.
Bertozzi, Carolyn, Yarema, Kevin J, and Mahal, Lara K. Sat .
"Glycoconjugates and methods". United States. https://www.osti.gov/servlets/purl/873035.
@article{osti_873035,
title = {Glycoconjugates and methods},
author = {Bertozzi, Carolyn and Yarema, Kevin J and Mahal, Lara K},
abstractNote = {Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
Works referenced in this record:
Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis
journal, May 1997
- Mahal, Lara K.; Yarema, Kevin J.; Bertozzi, Carolyn R.
- Science, Vol. 276, Issue 5315, p. 1125-1128
Synthesis of Novel Amphiphilic Compounds Containing Aza-12-crown-4 or D-Glucosamine and Their Ion Permeability
journal, January 1996
- Katoh, Akira; Ishida, Shin-ichi; Ohkanda, Junko
- HETEROCYCLES, Vol. 43, Issue 3