Title: Glycoconjugates and methods

Abstract

Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.

Inventors:

Bertozzi, Carolyn C ^[1]; Yarema, Kevin J ^[1]; Mahal, Lara K ^[2]

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+ Show Inventor Affiliations

1. Albany, CA
2. Berkeley, CA

Issue Date:

2008-04-01

Research Org.:

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

985675

Patent Number(s):

7351408

Application Number:

11/188,587

Assignee:

The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

C07H15/12 - attached to a nitrogen atom of the saccharide radical

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K49/1896 - {not provided for elsewhere, e.g. cells, viruses, ghosts, red blood cells, virus capsides}
A61K47/665 - {the pre-targeting system, clearing therapy or rescue therapy involving biotin-

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

A61P35/00 - Antineoplastic agents

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

C07H15/203 - Monocyclic carbocyclic rings other than cyclohexane rings

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K47/6901 - {Conjugates being cells, cell fragments, viruses, ghosts, red blood cells or viral vectors}
A61K51/1203 - {in a form not provided for by groups A61K51/1206 - A61K51/1296, e.g. cells, cell fragments, viruses, virus capsides, ghosts, red blood cells, viral vectors}

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

B82Y5/00 - Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K47/6415 - {Toxins or lectins, e.g. clostridial toxins or Pseudomonas exotoxins}
A61K49/103 - {the complex-forming compound being acyclic, e.g. DTPA}
A61K47/549 - {Sugars, nucleosides, nucleotides or nucleic acids}

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DOE Contract Number:  

AC03-76SF00098

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES