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Title: Glycoconjugates and methods

Abstract

Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.

Inventors:
 [1];  [1];  [2]
  1. Albany, CA
  2. Berkeley, CA
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
OSTI Identifier:
874780
Patent Number(s):
6458937
Application Number:
09/571527
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
glycoconjugates; methods; functionalized; contacting; cell; monosaccharide; incubating; conditions; internalizes; ii; biochemically; processes; saccharide; iii; conjugates; carrier; form; glycoconjugate; extracellularly; expresses; extracellular; comprising; selectively; reactive; functional; forming; products; expressed; agent; reacts; product; subject; compositions; cyto-compatible; monosaccharides; nitrogen; linked; surface; cells; saccharides; /999/

Citation Formats

Bertozzi, Carolyn, Yarema, Kevin J, and Mahal, Lara K. Glycoconjugates and methods. United States: N. p., 2002. Web.
Bertozzi, Carolyn, Yarema, Kevin J, & Mahal, Lara K. Glycoconjugates and methods. United States.
Bertozzi, Carolyn, Yarema, Kevin J, and Mahal, Lara K. Tue . "Glycoconjugates and methods". United States. https://www.osti.gov/servlets/purl/874780.
@article{osti_874780,
title = {Glycoconjugates and methods},
author = {Bertozzi, Carolyn and Yarema, Kevin J and Mahal, Lara K},
abstractNote = {Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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Works referenced in this record:

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journal, November 1992


New amino sugar analogues are incorporated at different rates into glycoproteins of mouse organs
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Biosynthetic Modulation of Sialic Acid-dependent Virus-Receptor Interactions of Two Primate Polyoma Viruses
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Biosynthesis of a nonphysiological sialic acid in different rat organs, using N-propanoyl-D-hexosamines as precursors.
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Biochemistry: Making Cells Selectively Sticky
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