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Title: Glycoconjugates and methods

Patent ·
OSTI ID:874780
 [1];  [1];  [2]
  1. Albany, CA
  2. Berkeley, CA
+ Show Author Affiliations

Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
DOE Contract Number:
AC03-76SF00098
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
US 6458937
Application Number:
09/571527
OSTI ID:
874780
Country of Publication:
United States
Language:
English

References (8)

Synthesis of potential inhibitors of hemagglutination by influenza virus: chemoenzymic preparation of N-5 analogs of N-acetylneuraminic acid. journal January 1993
Enzymatic transfer of a preassembled trisaccharide antigen to cell surfaces using a fucosyltransferase. journal November 1992
Sialic acid aldolase-catalyzed condensation of pyruvate and N-substituted mannosamine: A useful method for the synthesis of N-substituted sialic acids journal April 1997
New amino sugar analogues are incorporated at different rates into glycoproteins of mouse organs journal October 1993
Synthesis of Novel Amphiphilic Compounds Containing Aza-12-crown-4 or D-Glucosamine and Their Ion Permeability journal January 1996
Biosynthetic Modulation of Sialic Acid-dependent Virus-Receptor Interactions of Two Primate Polyoma Viruses journal January 1995
Biosynthesis of a nonphysiological sialic acid in different rat organs, using N-propanoyl-D-hexosamines as precursors. journal October 1992
Biochemistry: Making Cells Selectively Sticky journal September 1996

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Related Subjects

glycoconjugates
methods
functionalized
contacting
cell
monosaccharide
incubating
conditions
internalizes
ii
biochemically
processes
saccharide
iii
conjugates
carrier
form
glycoconjugate
extracellularly
expresses
extracellular
comprising
selectively
reactive
functional
forming
products
expressed
agent
reacts
product
subject
compositions
cyto-compatible
monosaccharides
nitrogen
linked
surface
cells
saccharides
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