Glycoconjugates and methods

Patent · Sat Jan 01 00:00:00 EST 2000

OSTI ID:873035

Bertozzi, Carolyn ^[1]; Yarema, Kevin J ^[1]; Mahal, Lara K ^[2]

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1. Albany, CA
2. Berkeley, CA

Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• DOE Contract Number: AC03-76SF00098
• Assignee: Regents of University of California (Oakland, CA)
• Patent Number(s): US 6075134
• OSTI ID: 873035
• Country of Publication: United States
• Language: English

Related Subjects

glycoconjugates
methods
functionalized
contacting
cell
monosaccharide
incubating
conditions
whereby
internalizes
ii
biochemically
processes
saccharide
iii
conjugates
carrier
form
glycoconjugate
extracellularly
expresses
extracellular
comprising
selectively
reactive
functional
forming
products
comprise
expressed
agent
reacts
product
subject
compositions
cyto-compatible
monosaccharides
nitrogen
linked
surface
cells
saccharides
conditions whereby
cell surface
reactive functional
cells comprising
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