Site directed recombination
Abstract
Enhanced homologous recombination is obtained by employing a consensus sequence which has been found to be associated with integration of repeat sequences, such as Alu and ID. The consensus sequence or sequence having a single transition mutation determines one site of a double break which allows for high efficiency of integration at the site. By introducing single or double stranded DNA having the consensus sequence flanking region joined to a sequence of interest, one can reproducibly direct integration of the sequence of interest at one or a limited number of sites. In this way, specific sites can be identified and homologous recombination achieved at the site by employing a second flanking sequence associated with a sequence proximal to the 3'-nick.
- Inventors:
-
- Los Altos, CA
- Issue Date:
- Research Org.:
- Genetic Information Research Institute (Palo Alto, CA)
- OSTI Identifier:
- 871268
- Patent Number(s):
- 5695977
- Assignee:
- Genetic Information Research Institute (Palo Alto, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-95ER62139
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- site; directed; recombination; enhanced; homologous; obtained; employing; consensus; sequence; found; associated; integration; repeat; sequences; alu; single; transition; mutation; determines; double; break; allows; efficiency; introducing; stranded; dna; flanking; region; joined; reproducibly; direct; limited; sites; specific; identified; achieved; proximal; -nick; stranded dna; double stranded; repeat sequences; repeat sequence; consensus sequence; /435/999/
Citation Formats
Jurka, Jerzy W. Site directed recombination. United States: N. p., 1997.
Web.
Jurka, Jerzy W. Site directed recombination. United States.
Jurka, Jerzy W. Wed .
"Site directed recombination". United States. https://www.osti.gov/servlets/purl/871268.
@article{osti_871268,
title = {Site directed recombination},
author = {Jurka, Jerzy W},
abstractNote = {Enhanced homologous recombination is obtained by employing a consensus sequence which has been found to be associated with integration of repeat sequences, such as Alu and ID. The consensus sequence or sequence having a single transition mutation determines one site of a double break which allows for high efficiency of integration at the site. By introducing single or double stranded DNA having the consensus sequence flanking region joined to a sequence of interest, one can reproducibly direct integration of the sequence of interest at one or a limited number of sites. In this way, specific sites can be identified and homologous recombination achieved at the site by employing a second flanking sequence associated with a sequence proximal to the 3'-nick.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1997},
month = {Wed Jan 01 00:00:00 EST 1997}
}
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