Designed proteins for pH switchable antibody purification
Abstract
Modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are provided. In some examples, a modified Fc-binding domain includes or consists of the amino acid sequence of any one of SEQ ID NOs: 4, 6, or 8. Nucleic acids encoding the modified Fc-binding domains or polypeptides including one or more of the modified Fc-binding domains are also provided. In some embodiments, the nucleic acids are included in a vector and may be operably linked to a promoter. Methods for purifying a polypeptide including one or more Fc regions are also provided. Kits and reagents including the modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are provided. In some examples, the modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are linked to a solid support.
- Inventors:
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1805652
- Patent Number(s):
- 10954275
- Application Number:
- 16/054,882
- Assignee:
- Triad National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- AC52-06NA25396
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 08/03/2018
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Jha, Roshan Kumar, Strauss, Charlie E., and Bradbury, Andrew M. Designed proteins for pH switchable antibody purification. United States: N. p., 2021.
Web.
Jha, Roshan Kumar, Strauss, Charlie E., & Bradbury, Andrew M. Designed proteins for pH switchable antibody purification. United States.
Jha, Roshan Kumar, Strauss, Charlie E., and Bradbury, Andrew M. Tue .
"Designed proteins for pH switchable antibody purification". United States. https://www.osti.gov/servlets/purl/1805652.
@article{osti_1805652,
title = {Designed proteins for pH switchable antibody purification},
author = {Jha, Roshan Kumar and Strauss, Charlie E. and Bradbury, Andrew M.},
abstractNote = {Modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are provided. In some examples, a modified Fc-binding domain includes or consists of the amino acid sequence of any one of SEQ ID NOs: 4, 6, or 8. Nucleic acids encoding the modified Fc-binding domains or polypeptides including one or more of the modified Fc-binding domains are also provided. In some embodiments, the nucleic acids are included in a vector and may be operably linked to a promoter. Methods for purifying a polypeptide including one or more Fc regions are also provided. Kits and reagents including the modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are provided. In some examples, the modified Fc-binding domain polypeptides or polypeptides including one or more modified Fc-binding domains are linked to a solid support.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 23 00:00:00 EDT 2021},
month = {Tue Mar 23 00:00:00 EDT 2021}
}
Works referenced in this record:
Binding Proteins to the Constant Region of Immunoglobulin G
patent-application, February 2015
- Baker, David; Fleishman, Sarel Jacob; Strauch, Eva-Maria
- US Patent Application 14/387699; 20150037312