Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis
Abstract
Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1082456
- Patent Number(s):
- 8329016
- Application Number:
- 12/551,047
- Assignee:
- Sandia Corporation (Albuquerque, NM)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 77 NANOSCIENCE AND NANOTECHNOLOGY
Citation Formats
Sommer, Gregory J., Hatch, Anson V., Singh, Anup K., and Wang, Ying-Chih. Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis. United States: N. p., 2012.
Web.
Sommer, Gregory J., Hatch, Anson V., Singh, Anup K., & Wang, Ying-Chih. Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis. United States.
Sommer, Gregory J., Hatch, Anson V., Singh, Anup K., and Wang, Ying-Chih. Tue .
"Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis". United States. https://www.osti.gov/servlets/purl/1082456.
@article{osti_1082456,
title = {Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis},
author = {Sommer, Gregory J. and Hatch, Anson V. and Singh, Anup K. and Wang, Ying-Chih},
abstractNote = {Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {12}
}
Works referenced in this record:
Integration of isoelectric focusing with multi-channel gel electrophoresis by using microfluidic pseudo-valves
journal, January 2007
- Das, Champak; Zhang, Jiyou; Denslow, Nancy D.
- Lab on a Chip, Vol. 7, Issue 12
On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008
- Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
- Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333