Engineered microcompartment protein and related methods and systems of engineering bacterial systems for non-native protein expression and purification

Yung, Mimi Cho; Carpenter, Timothy S.; Lee, Tek Hyung; Savage, David

Title: Engineered microcompartment protein and related methods and systems of engineering bacterial systems for non-native protein expression and purification

Abstract

Engineered microcompartment proteins, and related engineered microcompartment, vectors, cells compositions, methods and systems configured to provide within a cell one or more proteins non-native to the cell are described, wherein the one or more proteins non-native to the cell are contained in at least one engineered microcompartment within the cell.

Inventors:: Yung, Mimi Cho; Carpenter, Timothy S.; Lee, Tek Hyung; Savage, David

Issue Date:: 2020-08-11

Research Org.:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1735126

Patent Number(s):: 10738090

Application Number:: 16/219,781

Assignee:: Lawrence Livermore National Security, LLC (Livermore, CA); The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

Show more

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K2319/50 - containing protease site
C07K14/43572 - {from bees}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/96 - Stabilising an enzyme by forming an adduct or a composition

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/195 - from bacteria
C07K14/43563 - {from insects}
C07K2319/70 - containing domain for protein-protein interaction

Show less

DOE Contract Number:: AC52-07NA27344

Resource Type:: Patent

Resource Relation:: Patent File Date: 12/13/2018

Country of Publication:: United States

Language:: English

Citation Formats


                    Yung, Mimi Cho, Carpenter, Timothy S., Lee, Tek Hyung, and Savage, David. Engineered microcompartment protein and related methods and systems of engineering bacterial systems for non-native protein expression and purification.  United States: N. p., 2020. 
        Web.

Copy to clipboard


                    Yung, Mimi Cho, Carpenter, Timothy S., Lee, Tek Hyung, & Savage, David. Engineered microcompartment protein and related methods and systems of engineering bacterial systems for non-native protein expression and purification.  United States.

Copy to clipboard


                    Yung, Mimi Cho, Carpenter, Timothy S., Lee, Tek Hyung, and Savage, David. Tue .  
        "Engineered microcompartment protein and related methods and systems of engineering bacterial systems for non-native protein expression and purification".  United States.  https://www.osti.gov/servlets/purl/1735126.

Copy to clipboard


                    
@article{osti_1735126,

  title        = {Engineered microcompartment protein and related methods and systems of engineering bacterial systems for non-native protein expression and purification},

  author       = {Yung, Mimi Cho and Carpenter, Timothy S. and Lee, Tek Hyung and Savage, David},

  abstractNote = {Engineered microcompartment proteins, and related engineered microcompartment, vectors, cells compositions, methods and systems configured to provide within a cell one or more proteins non-native to the cell are described, wherein the one or more proteins non-native to the cell are contained in at least one engineered microcompartment within the cell.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2020},

  month        = {8}

}

Copy to clipboard

Patent:

View Patent

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction.
journal, March 1995

Dong, H.; Nilsson, L.; Kurland, C. G.
Journal of Bacteriology, Vol. 177, Issue 6
https://doi.org/10.1128/jb.177.6.1497-1504.1995

Synthetic biology and the development of tools for metabolic engineering
journal, May 2012

Keasling, Jay D.
Metabolic Engineering, Vol. 14, Issue 3
https://doi.org/10.1016/j.ymben.2012.01.004

Structural basis of enzyme encapsulation into a bacterial nanocompartment
journal, August 2008

Sutter, Markus; Boehringer, Daniel; Gutmann, Sascha
Nature Structural & Molecular Biology, Vol. 15, Issue 9
https://doi.org/10.1038/nsmb.1473

Growth and Localization of Polyhydroxybutyrate Granules in Ralstonia eutropha
journal, December 2011

Beeby, M.; Cho, M.; Stubbe, J.
Journal of Bacteriology, Vol. 194, Issue 5
https://doi.org/10.1128/JB.06125-11

Energetics of ion conduction through the K+ channel
journal, November 2001

Bernèche, Simon; Roux, Benoît
Nature, Vol. 414, Issue 6859
https://doi.org/10.1038/35102067

Encapsulins: molecular biology of the shell
journal, June 2017

Nichols, Robert J.; Cassidy-Amstutz, Caleb; Chaijarasphong, Thawatchai
Critical Reviews in Biochemistry and Molecular Biology, Vol. 52, Issue 5
https://doi.org/10.1080/10409238.2017.1337709

Crystal Structure of MraY, an Essential Membrane Enzyme for Bacterial Cell Wall Synthesis
journal, August 2013

Chung, B. C.; Zhao, J.; Gillespie, R. A.
Science, Vol. 341, Issue 6149
https://doi.org/10.1126/science.1236501

Peptide Nanopores and Lipid Bilayers: Interactions by Coarse-Grained Molecular-Dynamics Simulations
journal, May 2009

Klingelhoefer, Jochen W.; Carpenter, Timothy; Sansom, Mark S. P.
Biophysical Journal, Vol. 96, Issue 9
https://doi.org/10.1016/j.bpj.2009.01.046

Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications
journal, January 2012

Young, Carissa L.; Britton, Zachary T.; Robinson, Anne S.
Biotechnology Journal, Vol. 7, Issue 5
https://doi.org/10.1002/biot.201100155

Where did the BLOSUM62 alignment score matrix come from?
journal, August 2004

Eddy, Sean R.
Nature Biotechnology, Vol. 22, Issue 8
https://doi.org/10.1038/nbt0804-1035

Genetically Programmable Pathogen Sense and Destroy
journal, July 2013

Gupta, Saurabh; Bram, Eran E.; Weiss, Ron
ACS Synthetic Biology, Vol. 2, Issue 12
https://doi.org/10.1021/sb4000417

Purification of Polyhydroxybutyrate Synthase from Its Native Organism, Ralstonia eutropha : Implications for the Initiation and Elongation of Polymer Formation in Vivo
journal, March 2012

Cho, Mimi; Brigham, Christopher J.; Sinskey, Anthony J.
Biochemistry, Vol. 51, Issue 11
https://doi.org/10.1021/bi2013596

Pneumonia Due to Pseudomonas aeruginosa
journal, April 2011

Fujitani, Shigeki; Sun, Hsin-Yun; Yu, Victor L.
Chest, Vol. 139, Issue 4
https://doi.org/10.1378/chest.10-0166

OmpA: Gating and dynamics via molecular dynamics simulations
journal, September 2008

Khalid, Syma; Bond, Peter J.; Carpenter, Timothy
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1778, Issue 9
https://doi.org/10.1016/j.bbamem.2007.05.024

Identification of c-type cytochromes involved in anaerobic, bacterial U(IV) oxidation
journal, May 2008

Beller, Harry R.; Legler, Tina C.; Bourguet, Feliza
Biodegradation, Vol. 20, Issue 1
https://doi.org/10.1007/s10532-008-9198-y

Self-Assembly of a Simple Membrane Protein: Coarse-Grained Molecular Dynamics Simulations of the Influenza M2 Channel
journal, October 2008

Carpenter, Timothy; Bond, Peter J.; Khalid, Syma
Biophysical Journal, Vol. 95, Issue 8
https://doi.org/10.1529/biophysj.108.131078

The PduM Protein Is a Structural Component of the Microcompartments Involved in Coenzyme B12-Dependent 1,2-Propanediol Degradation by Salmonella enterica
journal, February 2012

Sinha, S.; Cheng, S.; Fan, C.
Journal of Bacteriology, Vol. 194, Issue 8
https://doi.org/10.1128/JB.06529-11

Overproduction of the toxic protein, bovine pancreatic DNasel, in Escherichia coli using a tightly controlled T7-promoter-based vector
journal, December 1993

Doherty, Aidan J.; Connolly, Bernard A.; Worrall, Andrew F.
Gene, Vol. 136, Issue 1-2
https://doi.org/10.1016/0378-1119(93)90491-K

Expression systems for heterologous production of antimicrobial peptides
journal, December 2012

Parachin, Nádia Skorupa; Mulder, Kelly Cristina; Viana, Antônio Américo Barbosa
Peptides, Vol. 38, Issue 2
https://doi.org/10.1016/j.peptides.2012.09.020

Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system
journal, March 2006

Giacalone, Matthew J.; Gentile, Angela M.; Lovitt, Brian T.
BioTechniques, Vol. 40, Issue 3
https://doi.org/10.2144/000112112

Toxin Synthesis by Clostridium difficile Is Regulated through Quorum Signaling
journal, February 2015

Darkoh, Charles; DuPont, Herbert L.; Norris, Steven J.
mBio, Vol. 6, Issue 2
https://doi.org/10.1128/mBio.02569-14

Studies on anticancer activities of antimicrobial peptides
journal, February 2008

Hoskin, David W.; Ramamoorthy, Ayyalusamy
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1778, Issue 2
https://doi.org/10.1016/j.bbamem.2007.11.008

Solution Structure of a Bacterial Microcompartment Targeting Peptide and Its Application in the Construction of an Ethanol Bioreactor
journal, November 2013

Lawrence, Andrew D.; Frank, Stefanie; Newnham, Sarah
ACS Synthetic Biology, Vol. 3, Issue 7
https://doi.org/10.1021/sb4001118

A tetrazolium-based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia
journal, September 1994

van de Loosdrecht, A. A.; Beelen, R. H. J.; Ossenkoppele, G. J.
Journal of Immunological Methods, Vol. 174, Issue 1-2
https://doi.org/10.1016/0022-1759(94)90034-5

Apidaecin-type peptides: Biodiversity, structure–function relationships and mode of action
journal, September 2006

Li, Wei-Fen; Ma, Guo-Xia; Zhou, Xu-Xia
Peptides, Vol. 27, Issue 9
https://doi.org/10.1016/j.peptides.2006.03.016

Human Health Effects of Trichloroethylene: Key Findings and Scientific Issues
journal, March 2013

Chiu, Weihsueh A.; Jinot, Jennifer; Scott, Cheryl Siegel
Environmental Health Perspectives, Vol. 121, Issue 3
https://doi.org/10.1289/ehp.1205879

Bacterial Quorum Sensing: Its Role in Virulence and Possibilities for Its Control
journal, November 2012

Rutherford, S. T.; Bassler, B. L.
Cold Spring Harbor Perspectives in Medicine, Vol. 2, Issue 11
https://doi.org/10.1101/cshperspect.a012427

Bacterial Microcompartment Organelles: Protein Shell Structure and Evolution
journal, April 2010

Yeates, Todd O.; Crowley, Christopher S.; Tanaka, Shiho
Annual Review of Biophysics, Vol. 39, Issue 1
https://doi.org/10.1146/annurev.biophys.093008.131418

Prediction of Blood-Brain Barrier Permeability from Molecular Dynamics Simulations
journal, January 2014

Carpenter, Timothy S.; Lau, Edmond Y.; Kirshner, Daniel A.
Biophysical Journal, Vol. 106, Issue 2
https://doi.org/10.1016/j.bpj.2013.11.533

A guide to choosing fluorescent proteins
journal, November 2005

Shaner, Nathan C.; Steinbach, Paul A.; Tsien, Roger Y.
Nature Methods, Vol. 2, Issue 12
https://doi.org/10.1038/nmeth819

Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines
journal, January 2011

Bermúdez-Humarán, Luis G.; Kharrat, Pascale; Chatel, Jean-Marc
Microbial Cell Factories, Vol. 10, Issue Suppl 1
https://doi.org/10.1186/1475-2859-10-S1-S4

Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli
journal, December 2005

Zhang, Guijin; Brokx, Stephen; Weiner, Joel H
Nature Biotechnology, Vol. 24, Issue 1, p. 100-104
https://doi.org/10.1038/nbt1174

Engineered Phagemids for Nonlytic, Targeted Antibacterial Therapies
journal, June 2015

Krom, Russell J.; Bhargava, Prerna; Lobritz, Michael A.
Nano Letters, Vol. 15, Issue 7
https://doi.org/10.1021/acs.nanolett.5b01943

Intramembrane-cleaving Proteases
journal, February 2009

Wolfe, Michael S.
Journal of Biological Chemistry, Vol. 284, Issue 21
https://doi.org/10.1074/jbc.R800039200

Purification and characterization of toluene 2-monooxygenase from Burkholderia cepacia G4
journal, October 1995

Newman, Lisa M.; Wackett, Lawrence P.
Biochemistry, Vol. 34, Issue 43
https://doi.org/10.1021/bi00043a012

Antimicrobial Peptides Targeting Gram-negative Pathogens, Produced and Delivered by Lactic Acid Bacteria
journal, July 2013

Volzing, Katherine; Borrero, Juan; Sadowsky, Michael J.
ACS Synthetic Biology, Vol. 2, Issue 11
https://doi.org/10.1021/sb4000367

Directed Evolution of a Protein Container
journal, February 2011

Worsdorfer, B.; Woycechowsky, K. J.; Hilvert, D.
Science, Vol. 331, Issue 6017
https://doi.org/10.1126/science.1199081

Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli
journal, April 2017

Yung, Mimi C.; Bourguet, Feliza A.; Carpenter, Timothy S.
Microbial Cell Factories, Vol. 16, Issue 1
https://doi.org/10.1186/s12934-017-0685-x

Protein-based organelles in bacteria: carboxysomes and related microcompartments
journal, August 2008

Yeates, Todd O.; Kerfeld, Cheryl A.; Heinhorst, Sabine
Nature Reviews Microbiology, Vol. 6, Issue 9
https://doi.org/10.1038/nrmicro1913

Short Proline-Rich Antimicrobial Peptides Inhibit Either the Bacterial 70S Ribosome or the Assembly of its Large 50S Subunit
journal, October 2015

Krizsan, Andor; Prahl, Caroline; Goldbach, Tina
ChemBioChem, Vol. 16, Issue 16
https://doi.org/10.1002/cbic.201500375

Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement
journal, April 2010

Parsons, Joshua B.; Frank, Stefanie; Bhella, David
Molecular Cell, Vol. 38, Issue 2
https://doi.org/10.1016/j.molcel.2010.04.008

Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening
journal, June 2010

Harrison, Joe J.; Stremick, Carol A.; Turner, Raymond J.
Nature Protocols, Vol. 5, Issue 7
https://doi.org/10.1038/nprot.2010.71

On the active site of proteases. III. Mapping the active site of papain; specific peptide inhibitors of papain
journal, September 1968

Schechter, Israel; Berger, Arieh
Biochemical and Biophysical Research Communications, Vol. 32, Issue 5
https://doi.org/10.1016/0006-291X(68)90326-4

From antimicrobial to anticancer peptides. A review
journal, January 2013

Gaspar, Diana; Veiga, A. Salomé; Castanho, Miguel A. R. B.
Frontiers in Microbiology, Vol. 4
https://doi.org/10.3389/fmicb.2013.00294

Hidden Markov model speed heuristic and iterative HMM search procedure
journal, August 2010

Johnson, L. Steven; Eddy, Sean R.; Portugaly, Elon
BMC Bioinformatics, Vol. 11, Issue 1
https://doi.org/10.1186/1471-2105-11-431

Engineering microbes to sense and eradicate Pseudomonas aeruginosa , a human pathogen
journal, January 2011

Saeidi, Nazanin; Wong, Choon Kit; Lo, Tat‐Ming
Molecular Systems Biology, Vol. 7, Issue 1
https://doi.org/10.1038/msb.2011.55

Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli
journal, January 2011

Menzella, Hugo G.
Microbial Cell Factories, Vol. 10, Issue 1
https://doi.org/10.1186/1475-2859-10-15

Extracellular DNA Required for Bacterial Biofilm Formation
journal, February 2002

Whitchurch, C. B.
Science, Vol. 295, Issue 5559
https://doi.org/10.1126/science.295.5559.1487

Automated design of synthetic ribosome binding sites to control protein expression
journal, October 2009

Salis, Howard M.; Mirsky, Ethan A.; Voigt, Christopher A.
Nature Biotechnology, Vol. 27, Issue 10, p. 946-950
https://doi.org/10.1038/nbt.1568

A multidomain outer membrane protein from Pasteurella multocida: Modelling and simulation studies of PmOmpA
journal, November 2007

Carpenter, Timothy; Khalid, Syma; Sansom, Mark S. P.
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1768, Issue 11
https://doi.org/10.1016/j.bbamem.2007.07.025

Antimicrobial peptides and their interaction with biofilms of medically relevant bacteria
journal, May 2016

Batoni, Giovanna; Maisetta, Giuseppantonio; Esin, Semih
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1858, Issue 5
https://doi.org/10.1016/j.bbamem.2015.10.013

Directed Evolution of Toluene ortho-Monooxygenase for Enhanced 1-Naphthol Synthesis and Chlorinated Ethene Degradation
journal, January 2002

Canada, K. A.; Iwashita, S.; Shim, H.
Journal of Bacteriology, Vol. 184, Issue 2
https://doi.org/10.1128/JB.184.2.344-349.2002

Anion and Carboxylic Acid Binding to Monotopic and Ditopic Amidopyridine Macrocycles
journal, July 2008

Korendovych, Ivan V.; Cho, Mimi; Makhlynets, Olga V.
The Journal of Organic Chemistry, Vol. 73, Issue 13
https://doi.org/10.1021/jo800128r

X174 cistron A protein is a multifunctional enzyme in DNA replication
journal, August 1977

Eisenberg, S.; Griffith, J.; Kornberg, A.
Proceedings of the National Academy of Sciences, Vol. 74, Issue 8
https://doi.org/10.1073/pnas.74.8.3198

Short N-terminal sequences package proteins into bacterial microcompartments
journal, March 2010

Fan, C.; Cheng, S.; Liu, Y.
Proceedings of the National Academy of Sciences, Vol. 107, Issue 16
https://doi.org/10.1073/pnas.0913199107

Anion Binding to Monotopic and Ditopic Macrocyclic Amides
journal, July 2006

Korendovych, Ivan V.; Cho, Mimi; Butler, Phillip L.
Organic Letters, Vol. 8, Issue 15
https://doi.org/10.1021/ol060786r

Antimicrobial peptides: premises and promises
journal, December 2004

Reddy, K. V. R.; Yedery, R. D.; Aranha, C.
International Journal of Antimicrobial Agents, Vol. 24, Issue 6
https://doi.org/10.1016/j.ijantimicag.2004.09.005

On the size of the active site in proteases. I. Papain
journal, April 1967

Schechter, Israel; Berger, Arieh
Biochemical and Biophysical Research Communications, Vol. 27, Issue 2, p. 157-162
https://doi.org/10.1016/S0006-291X(67)80055-X

GABAA receptor target of tetramethylenedisulfotetramine
journal, May 2014

Zhao, C.; Hwang, S. H.; Buchholz, B. A.
Proceedings of the National Academy of Sciences, Vol. 111, Issue 23
https://doi.org/10.1073/pnas.1407379111

Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform
journal, September 2014

Moon, Hyojin; Lee, Jisu; Min, Junseon
Biomacromolecules, Vol. 15, Issue 10
https://doi.org/10.1021/bm501066m

Alginate Lyase: Review of Major Sources and Enzyme Characteristics, Structure-Function Analysis, Biological Roles, and Applications
journal, October 2000

Wong, Thiang Yian; Preston, Lori A.; Schiller, Neal L.
Annual Review of Microbiology, Vol. 54, Issue 1
https://doi.org/10.1146/annurev.micro.54.1.289

Engineering Controllable Protein Degradation
journal, June 2006

McGinness, Kathleen E.; Baker, Tania A.; Sauer, Robert T.
Molecular Cell, Vol. 22, Issue 5
https://doi.org/10.1016/j.molcel.2006.04.027

Employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in Escherichia coli
journal, July 2016

Lee, Matthew J.; Brown, Ian R.; Juodeikis, Rokas
Metabolic Engineering, Vol. 36
https://doi.org/10.1016/j.ymben.2016.02.007

Identification of common molecular subsequences
journal, March 1981

Smith, T. F.; Waterman, M. S.
Journal of Molecular Biology, Vol. 147, Issue 1, p. 195-197
https://doi.org/10.1016/0022-2836(81)90087-5

Prokaryotic nanocompartments form synthetic organelles in a eukaryote
journal, April 2018

Lau, Yu Heng; Giessen, Tobias W.; Altenburg, Wiggert J.
Nature Communications, Vol. 9, Issue 1
https://doi.org/10.1038/s41467-018-03768-x

A synthetic system for expression of components of a bacterial microcompartment
journal, September 2013

Sargent, F.; Davidson, F. A.; Kelly, C. L.
Microbiology, Vol. 159, Issue Pt_11
https://doi.org/10.1099/mic.0.069922-0

A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method
journal, October 2014

Axen, Seth D.; Erbilgin, Onur; Kerfeld, Cheryl A.
PLoS Computational Biology, Vol. 10, Issue 10
https://doi.org/10.1371/journal.pcbi.1003898

Activation of RegB endoribonuclease by S1 ribosomal protein requires an 11 nt conserved sequence
journal, November 2006

Durand, Sylvain; Richard, Graziella; Bisaglia, Marco
Nucleic Acids Research, Vol. 34, Issue 22
https://doi.org/10.1093/nar/gkl911

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997

Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A.
Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402
https://doi.org/10.1093/nar/25.17.3389

The expanding scope of antimicrobial peptide structures and their modes of action
journal, September 2011

Nguyen, Leonard T.; Haney, Evan F.; Vogel, Hans J.
Trends in Biotechnology, Vol. 29, Issue 9
https://doi.org/10.1016/j.tibtech.2011.05.001

Identification of a Possible Secondary Picrotoxin-Binding Site on the GABA _A Receptor
journal, September 2013

Carpenter, Timothy S.; Lau, Edmond Y.; Lightstone, Felice C.
Chemical Research in Toxicology, Vol. 26, Issue 10
https://doi.org/10.1021/tx400167b

Characterization of De Novo Synthesized GPCRs Supported in Nanolipoprotein Discs
journal, September 2012

Gao, Tingjuan; Petrlova, Jitka; He, Wei
PLoS ONE, Vol. 7, Issue 9
https://doi.org/10.1371/journal.pone.0044911

Simulations of the BM2 Proton Channel Transmembrane Domain from Influenza Virus B
journal, October 2009

Rouse, Sarah L.; Carpenter, Timothy; Stansfeld, Phillip J.
Biochemistry, Vol. 48, Issue 42
https://doi.org/10.1021/bi901166n

Designer microbes for biosynthesis
journal, October 2014

Quin, Maureen B.; Schmidt-Dannert, Claudia
Current Opinion in Biotechnology, Vol. 29
https://doi.org/10.1016/j.copbio.2014.02.014

Design of orthogonal genetic switches based on a crosstalk map of σs, anti‐σs, and promoters
journal, January 2013

Rhodius, Virgil A.; Segall‐Shapiro, Thomas H.; Sharon, Brian D.
Molecular Systems Biology, Vol. 9, Issue 1
https://doi.org/10.1038/msb.2013.58

Biological Properties of Structurally Related α-Helical Cationic Antimicrobial Peptides
journal, April 1999

Scott, Monisha G.; Yan, Hong; Hancock, Robert E. W.
Infection and Immunity, Vol. 67, Issue 4
https://doi.org/10.1128/IAI.67.4.2005-2009.1999

Trichloroethylene oxidation by purified toluene 2-monooxygenase: products, kinetics, and turnover-dependent inactivation.
journal, January 1997

Newman, L. M.; Wackett, L. P.
Journal of Bacteriology, Vol. 179, Issue 1
https://doi.org/10.1128/jb.179.1.90-96.1997

The NRF2-KEAP1 Pathway Is an Early Responsive Gene Network in Arsenic Exposed Lymphoblastoid Cells
journal, February 2014

Córdova, Emilio J.; Martínez-Hernández, Angélica; Uribe-Figueroa, Laura
PLoS ONE, Vol. 9, Issue 2
https://doi.org/10.1371/journal.pone.0088069

Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin
journal, June 2016

Cassidy-Amstutz, Caleb; Oltrogge, Luke; Going, Catherine C.
Biochemistry, Vol. 55, Issue 24
https://doi.org/10.1021/acs.biochem.6b00294

A Role for Loop F in Modulating GABA Binding Affinity in the GABAA Receptor
journal, September 2012

Carpenter, Timothy S.; Lau, Edmond Y.; Lightstone, Felice C.
Journal of Molecular Biology, Vol. 422, Issue 2
https://doi.org/10.1016/j.jmb.2012.05.025

Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.
journal, January 1989

Zylstra, G. J.; Wackett, L. P.; Gibson, D. T.
Applied and Environmental Microbiology, Vol. 55, Issue 12
https://doi.org/10.1128/aem.55.12.3162-3166.1989

Methods and systems for engineering bacterial systems to shield toxicity during non-native protein expression and purification
patent, December 2018

Yung, Mimi Cho; Bourguet, Feliza A.; Carpenter, Tim
US Patent Document 10,155,951
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10155951

Identification of Critical Amino Acids within the Nucleoprotein of Tacaribe Virus Important for Anti-interferon Activity
journal, February 2013

Harmon, Brooke; Kozina, Carol; Maar, Dianna
Journal of Biological Chemistry, Vol. 288, Issue 12
https://doi.org/10.1074/jbc.M112.444760

Trichloroethylene degradation and mineralization by pseudomonads and Methylosinus trichosporium OB3b
journal, March 1996

Sun, A. K.; Wood, T. K.
Applied Microbiology and Biotechnology, Vol. 45, Issue 1-2
https://doi.org/10.1007/s002530050679

Reprogramming Microbes to Be Pathogen-Seeking Killers
journal, September 2013

Hwang, In Young; Tan, Mui Hua; Koh, Elvin
ACS Synthetic Biology, Vol. 3, Issue 4
https://doi.org/10.1021/sb400077j

Antimicrobial Peptide Therapeutics for Cystic Fibrosis
journal, June 2005

Zhang, L.; Parente, J.; Harris, S. M.
Antimicrobial Agents and Chemotherapy, Vol. 49, Issue 7
https://doi.org/10.1128/AAC.49.7.2921-2927.2005

Cell-Free Expression of Soluble and Membrane Proteins in an Array Device for Drug Screening
journal, August 2010

Khnouf, Ruba; Olivero, Daniel; Jin, Shouguang
Analytical Chemistry, Vol. 82, Issue 16
https://doi.org/10.1021/ac1015479

Shotgun Proteomic Analysis Unveils Survival and Detoxification Strategies by Caulobacter crescentus during Exposure to Uranium, Chromium, and Cadmium
journal, March 2014

Yung, Mimi C.; Ma, Jincai; Salemi, Michelle R.
Journal of Proteome Research, Vol. 13, Issue 4
https://doi.org/10.1021/pr400880s

Addition of Aromatic Substrates Restores Trichloroethylene Degradation Activity in Pseudomonas putida F1
journal, May 2004

Morono, Y.; Unno, H.; Tanji, Y.
Applied and Environmental Microbiology, Vol. 70, Issue 5
https://doi.org/10.1128/AEM.70.5.2830-2835.2004

Molecular approaches in bioremediation
journal, December 2008

Wood, Thomas K.
Current Opinion in Biotechnology, Vol. 19, Issue 6
https://doi.org/10.1016/j.copbio.2008.10.003

Systems biology approaches to bioremediation
journal, December 2008

de Lorenzo, Víctor
Current Opinion in Biotechnology, Vol. 19, Issue 6
https://doi.org/10.1016/j.copbio.2008.10.004

Bacteriophage endolysins as novel antimicrobials
journal, October 2012

Schmelcher, Mathias; Donovan, David M.; Loessner, Martin J.
Future Microbiology, Vol. 7, Issue 10
https://doi.org/10.2217/fmb.12.97

Searching protein sequence libraries: Comparison of the sensitivity and selectivity of the Smith-Waterman and FASTA algorithms
journal, November 1991

Pearson, William R.
Genomics, Vol. 11, Issue 3
https://doi.org/10.1016/0888-7543(91)90071-L

Controlling the diameter, monodispersity, and solubility of ApoA1 nanolipoprotein particles using telodendrimer chemistry: Size and Stability of NLPs
journal, June 2013

He, Wei; Luo, Juntao; Bourguet, Feliza
Protein Science, Vol. 22, Issue 8
https://doi.org/10.1002/pro.2292

Quantifying Interactions of a Membrane Protein Embedded in a Lipid Nanodisc using Fluorescence Correlation Spectroscopy
journal, January 2014

Ly, Sonny; Bourguet, Feliza; Fischer, Nicholas O.
Biophysical Journal, Vol. 106, Issue 2
https://doi.org/10.1016/j.bpj.2013.12.014

Formation and detoxification of reactive intermediates in the metabolism of chlorinated ethenes
journal, February 2001

van Hylckama Vlieg, Johan E. T.; Janssen, Dick B.
Journal of Biotechnology, Vol. 85, Issue 2
https://doi.org/10.1016/S0168-1656(00)00364-3

Characterizing diffusion dynamics of a membrane protein associated with nanolipoproteins using fluorescence correlation spectroscopy
journal, January 2011

Gao, Tingjuan; Blanchette, Craig D.; He, Wei
Protein Science, Vol. 20, Issue 2
https://doi.org/10.1002/pro.577

Low dose radiation response curves, networks and pathways in human lymphoblastoid cells exposed from 1 to 10cGy of acute gamma radiation
journal, June 2011

Wyrobek, A. J.; Manohar, C. F.; Krishnan, V. V.
Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 722, Issue 2
https://doi.org/10.1016/j.mrgentox.2011.03.002

Degradation of trichloroethylene by Pseudomonas cepacia G4 and the constitutive mutant strain G4 5223 PR1 in aquifer microcosms.
journal, January 1993

Krumme, M. L.; Timmis, K. N.; Dwyer, D. F.
Applied and Environmental Microbiology, Vol. 59, Issue 8
https://doi.org/10.1128/aem.59.8.2746-2749.1993

Engineered Protein Nano-Compartments for Targeted Enzyme Localization
journal, March 2012

Choudhary, Swati; Quin, Maureen B.; Sanders, Mark A.
PLoS ONE, Vol. 7, Issue 3
https://doi.org/10.1371/journal.pone.0033342

A rapid flow cytometry assay for the relative quantification of protein encapsulation into bacterial microcompartments
journal, January 2014

Kim, Edward Y.; Tullman-Ercek, Danielle
Biotechnology Journal, Vol. 9, Issue 3
https://doi.org/10.1002/biot.201300391

The second wave of synthetic biology: from modules to systems
journal, June 2009

Purnick, Priscilla E. M.; Weiss, Ron
Nature Reviews Molecular Cell Biology, Vol. 10, Issue 6
https://doi.org/10.1038/nrm2698

Metallation and mismetallation of iron and manganese proteins in vitro and in vivo: the class I ribonucleotide reductases as a case study
journal, January 2012

Cotruvo, Jr, Joseph A.; Stubbe, JoAnne
Metallomics, Vol. 4, Issue 10
https://doi.org/10.1039/c2mt20142a

Metabolic pathway engineering to enhance aerobic degradation of chlorinated ethenes and to reduce their toxicity by cloning a novel glutathione S-transferase, an evolved toluene o-monooxygenase, and gamma-glutamylcysteine synthetase
journal, May 2004

Rui, Lingyun; Kwon, Young Man; Reardon, Kenneth F.
Environmental Microbiology, Vol. 6, Issue 5
https://doi.org/10.1111/j.1462-2920.2004.00586.x

APD3: the antimicrobial peptide database as a tool for research and education
journal, November 2015

Wang, Guangshun; Li, Xia; Wang, Zhe
Nucleic Acids Research, Vol. 44, Issue D1
https://doi.org/10.1093/nar/gkv1278

Aerobic degradation of polychlorinated biphenyls
journal, December 2004

Pieper, Dietmar H.
Applied Microbiology and Biotechnology, Vol. 67, Issue 2
https://doi.org/10.1007/s00253-004-1810-4

Mechanisms of action on Escherichia coli of cecropin P1 and PR-39, two antibacterial peptides from pig intestine.
journal, January 1993

Boman, H. G.; Agerberth, B.; Boman, A.
Infection and Immunity, Vol. 61, Issue 7
https://doi.org/10.1128/iai.61.7.2978-2984.1993

Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins
journal, April 2015

Mamat, Uwe; Wilke, Kathleen; Bramhill, David
Microbial Cell Factories, Vol. 14, Issue 1
https://doi.org/10.1186/s12934-015-0241-5

Antibacterial peptides for therapeutic use: obstacles and realistic outlook
journal, October 2006

Marr, A.; Gooderham, W.; Hancock, R.
Current Opinion in Pharmacology, Vol. 6, Issue 5
https://doi.org/10.1016/j.coph.2006.04.006

Design, construction and characterization of a set of insulated bacterial promoters
journal, September 2010

Davis, J. H.; Rubin, A. J.; Sauer, R. T.
Nucleic Acids Research, Vol. 39, Issue 3, p. 1131-1141
https://doi.org/10.1093/nar/gkq810

Cytotoxicity Associated with Trichloroethylene Oxidation in Burkholderia cepacia G4
journal, May 2001

Yeager, C. M.; Bottomley, P. J.; Arp, D. J.
Applied and Environmental Microbiology, Vol. 67, Issue 5
https://doi.org/10.1128/AEM.67.5.2107-2115.2001

Similar Records in DOE Patents and OSTI.GOV collections:

Methods and systems for engineering bacterial systems to shield toxicity during non-native protein expression and purification

Patent Yung, Mimi Cho; Bourguet, Feliza A.; Carpenter, Timothy S.; ...

The invention described herein relates to methods, systems, compositions and cells to provide one or more toxic non-native proteins in a cell, wherein the one or more toxic non-native proteins are contained in at least one empty microcompartment within the cell.
Full Text Available
Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Patent Chromy, Brett A; Henderson, Paul; Hoeprich, Jr., Paul D

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.
Full Text Available
Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Patent Chromy, Brett A.; Henderson, Paul; Hoeprich, Jr, Paul D.

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.
Full Text Available
Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Patent Chromy, Brett A.; Henderson, Paul; Hoeprich, Jr, Paul D.

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.
Full Text Available
Modified bacterial microcompartment shell proteins

Patent Hagen, Andrew R.; Kerfeld, Cheryl A.; Sloan, Nancy B.; ...

The present invention provides for a fusion protein comprising (1) a bacterial microcompartment (BMC) shell protein comprising one or more subunit, and (2) a first component of a specific-binding pair, operably linked to the BMC shell protein such that the first component faces (i) a lumen (inside) side, or (ii) outside of a BMC shell formed incorporating the fusion protein and the fusion protein does not disrupt or prevent the folding of the BMC shell protein or the ability of the BMC shell protein to integrate with other BMC shell proteins into a BMC shell; wherein the first component ismore »« less
Full Text Available

Similar Records

Title: Engineered microcompartment protein and related methods and systems of engineering bacterial systems for non-native protein expression and purification

Abstract

Citation Formats

Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction. journal, March 1995

Synthetic biology and the development of tools for metabolic engineering journal, May 2012

Structural basis of enzyme encapsulation into a bacterial nanocompartment journal, August 2008

Growth and Localization of Polyhydroxybutyrate Granules in Ralstonia eutropha journal, December 2011

Energetics of ion conduction through the K+ channel journal, November 2001

Encapsulins: molecular biology of the shell journal, June 2017

Crystal Structure of MraY, an Essential Membrane Enzyme for Bacterial Cell Wall Synthesis journal, August 2013

Peptide Nanopores and Lipid Bilayers: Interactions by Coarse-Grained Molecular-Dynamics Simulations journal, May 2009

Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications journal, January 2012

Where did the BLOSUM62 alignment score matrix come from? journal, August 2004

Genetically Programmable Pathogen Sense and Destroy journal, July 2013

Purification of Polyhydroxybutyrate Synthase from Its Native Organism, Ralstonia eutropha : Implications for the Initiation and Elongation of Polymer Formation in Vivo journal, March 2012

Pneumonia Due to Pseudomonas aeruginosa journal, April 2011

OmpA: Gating and dynamics via molecular dynamics simulations journal, September 2008

Identification of c-type cytochromes involved in anaerobic, bacterial U(IV) oxidation journal, May 2008

Self-Assembly of a Simple Membrane Protein: Coarse-Grained Molecular Dynamics Simulations of the Influenza M2 Channel journal, October 2008

The PduM Protein Is a Structural Component of the Microcompartments Involved in Coenzyme B12-Dependent 1,2-Propanediol Degradation by Salmonella enterica journal, February 2012

Overproduction of the toxic protein, bovine pancreatic DNasel, in Escherichia coli using a tightly controlled T7-promoter-based vector journal, December 1993

Expression systems for heterologous production of antimicrobial peptides journal, December 2012

Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system journal, March 2006

Toxin Synthesis by Clostridium difficile Is Regulated through Quorum Signaling journal, February 2015

Studies on anticancer activities of antimicrobial peptides journal, February 2008

Solution Structure of a Bacterial Microcompartment Targeting Peptide and Its Application in the Construction of an Ethanol Bioreactor journal, November 2013

A tetrazolium-based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia journal, September 1994

Apidaecin-type peptides: Biodiversity, structure–function relationships and mode of action journal, September 2006

Human Health Effects of Trichloroethylene: Key Findings and Scientific Issues journal, March 2013

Bacterial Quorum Sensing: Its Role in Virulence and Possibilities for Its Control journal, November 2012

Bacterial Microcompartment Organelles: Protein Shell Structure and Evolution journal, April 2010

Prediction of Blood-Brain Barrier Permeability from Molecular Dynamics Simulations journal, January 2014

A guide to choosing fluorescent proteins journal, November 2005

Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines journal, January 2011

Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli journal, December 2005

Engineered Phagemids for Nonlytic, Targeted Antibacterial Therapies journal, June 2015

Intramembrane-cleaving Proteases journal, February 2009

Purification and characterization of toluene 2-monooxygenase from Burkholderia cepacia G4 journal, October 1995

Antimicrobial Peptides Targeting Gram-negative Pathogens, Produced and Delivered by Lactic Acid Bacteria journal, July 2013

Directed Evolution of a Protein Container journal, February 2011

Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli journal, April 2017

Protein-based organelles in bacteria: carboxysomes and related microcompartments journal, August 2008

Short Proline-Rich Antimicrobial Peptides Inhibit Either the Bacterial 70S Ribosome or the Assembly of its Large 50S Subunit journal, October 2015

Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement journal, April 2010

Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening journal, June 2010

On the active site of proteases. III. Mapping the active site of papain; specific peptide inhibitors of papain journal, September 1968

From antimicrobial to anticancer peptides. A review journal, January 2013

Hidden Markov model speed heuristic and iterative HMM search procedure journal, August 2010

Engineering microbes to sense and eradicate Pseudomonas aeruginosa , a human pathogen journal, January 2011

Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli journal, January 2011

Extracellular DNA Required for Bacterial Biofilm Formation journal, February 2002

Automated design of synthetic ribosome binding sites to control protein expression journal, October 2009

A multidomain outer membrane protein from Pasteurella multocida: Modelling and simulation studies of PmOmpA journal, November 2007

Antimicrobial peptides and their interaction with biofilms of medically relevant bacteria journal, May 2016

Directed Evolution of Toluene ortho-Monooxygenase for Enhanced 1-Naphthol Synthesis and Chlorinated Ethene Degradation journal, January 2002

Anion and Carboxylic Acid Binding to Monotopic and Ditopic Amidopyridine Macrocycles journal, July 2008

X174 cistron A protein is a multifunctional enzyme in DNA replication journal, August 1977

Short N-terminal sequences package proteins into bacterial microcompartments journal, March 2010

Anion Binding to Monotopic and Ditopic Macrocyclic Amides journal, July 2006

Antimicrobial peptides: premises and promises journal, December 2004

On the size of the active site in proteases. I. Papain journal, April 1967

GABAA receptor target of tetramethylenedisulfotetramine journal, May 2014

Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform journal, September 2014

Alginate Lyase: Review of Major Sources and Enzyme Characteristics, Structure-Function Analysis, Biological Roles, and Applications journal, October 2000

Engineering Controllable Protein Degradation journal, June 2006

Employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in Escherichia coli journal, July 2016

Identification of common molecular subsequences journal, March 1981

Prokaryotic nanocompartments form synthetic organelles in a eukaryote journal, April 2018

A synthetic system for expression of components of a bacterial microcompartment journal, September 2013

A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method journal, October 2014

Activation of RegB endoribonuclease by S1 ribosomal protein requires an 11 nt conserved sequence journal, November 2006

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs journal, September 1997

The expanding scope of antimicrobial peptide structures and their modes of action journal, September 2011

Identification of a Possible Secondary Picrotoxin-Binding Site on the GABA A Receptor journal, September 2013

Characterization of De Novo Synthesized GPCRs Supported in Nanolipoprotein Discs journal, September 2012

Simulations of the BM2 Proton Channel Transmembrane Domain from Influenza Virus B journal, October 2009

Designer microbes for biosynthesis journal, October 2014

Design of orthogonal genetic switches based on a crosstalk map of σs, anti‐σs, and promoters journal, January 2013

Biological Properties of Structurally Related α-Helical Cationic Antimicrobial Peptides journal, April 1999

Trichloroethylene oxidation by purified toluene 2-monooxygenase: products, kinetics, and turnover-dependent inactivation. journal, January 1997

Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction.
journal, March 1995

Synthetic biology and the development of tools for metabolic engineering
journal, May 2012

Structural basis of enzyme encapsulation into a bacterial nanocompartment
journal, August 2008

Growth and Localization of Polyhydroxybutyrate Granules in Ralstonia eutropha
journal, December 2011

Energetics of ion conduction through the K+ channel
journal, November 2001

Encapsulins: molecular biology of the shell
journal, June 2017

Crystal Structure of MraY, an Essential Membrane Enzyme for Bacterial Cell Wall Synthesis
journal, August 2013

Peptide Nanopores and Lipid Bilayers: Interactions by Coarse-Grained Molecular-Dynamics Simulations
journal, May 2009

Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications
journal, January 2012

Where did the BLOSUM62 alignment score matrix come from?
journal, August 2004

Genetically Programmable Pathogen Sense and Destroy
journal, July 2013

Purification of Polyhydroxybutyrate Synthase from Its Native Organism, Ralstonia eutropha : Implications for the Initiation and Elongation of Polymer Formation in Vivo
journal, March 2012

Pneumonia Due to Pseudomonas aeruginosa
journal, April 2011

OmpA: Gating and dynamics via molecular dynamics simulations
journal, September 2008

Identification of c-type cytochromes involved in anaerobic, bacterial U(IV) oxidation
journal, May 2008

Self-Assembly of a Simple Membrane Protein: Coarse-Grained Molecular Dynamics Simulations of the Influenza M2 Channel
journal, October 2008

The PduM Protein Is a Structural Component of the Microcompartments Involved in Coenzyme B12-Dependent 1,2-Propanediol Degradation by Salmonella enterica
journal, February 2012

Overproduction of the toxic protein, bovine pancreatic DNasel, in Escherichia coli using a tightly controlled T7-promoter-based vector
journal, December 1993

Expression systems for heterologous production of antimicrobial peptides
journal, December 2012

Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system
journal, March 2006

Toxin Synthesis by Clostridium difficile Is Regulated through Quorum Signaling
journal, February 2015

Studies on anticancer activities of antimicrobial peptides
journal, February 2008

Solution Structure of a Bacterial Microcompartment Targeting Peptide and Its Application in the Construction of an Ethanol Bioreactor
journal, November 2013

A tetrazolium-based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia
journal, September 1994

Apidaecin-type peptides: Biodiversity, structure–function relationships and mode of action
journal, September 2006

Human Health Effects of Trichloroethylene: Key Findings and Scientific Issues
journal, March 2013

Bacterial Quorum Sensing: Its Role in Virulence and Possibilities for Its Control
journal, November 2012

Bacterial Microcompartment Organelles: Protein Shell Structure and Evolution
journal, April 2010

Prediction of Blood-Brain Barrier Permeability from Molecular Dynamics Simulations
journal, January 2014

A guide to choosing fluorescent proteins
journal, November 2005

Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines
journal, January 2011

Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli
journal, December 2005

Engineered Phagemids for Nonlytic, Targeted Antibacterial Therapies
journal, June 2015

Intramembrane-cleaving Proteases
journal, February 2009

Purification and characterization of toluene 2-monooxygenase from Burkholderia cepacia G4
journal, October 1995

Antimicrobial Peptides Targeting Gram-negative Pathogens, Produced and Delivered by Lactic Acid Bacteria
journal, July 2013

Directed Evolution of a Protein Container
journal, February 2011

Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli
journal, April 2017

Protein-based organelles in bacteria: carboxysomes and related microcompartments
journal, August 2008

Short Proline-Rich Antimicrobial Peptides Inhibit Either the Bacterial 70S Ribosome or the Assembly of its Large 50S Subunit
journal, October 2015

Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement
journal, April 2010

Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening
journal, June 2010

On the active site of proteases. III. Mapping the active site of papain; specific peptide inhibitors of papain
journal, September 1968

From antimicrobial to anticancer peptides. A review
journal, January 2013

Hidden Markov model speed heuristic and iterative HMM search procedure
journal, August 2010

Engineering microbes to sense and eradicate Pseudomonas aeruginosa , a human pathogen
journal, January 2011

Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli
journal, January 2011

Extracellular DNA Required for Bacterial Biofilm Formation
journal, February 2002

Automated design of synthetic ribosome binding sites to control protein expression
journal, October 2009

A multidomain outer membrane protein from Pasteurella multocida: Modelling and simulation studies of PmOmpA
journal, November 2007

Antimicrobial peptides and their interaction with biofilms of medically relevant bacteria
journal, May 2016

Directed Evolution of Toluene ortho-Monooxygenase for Enhanced 1-Naphthol Synthesis and Chlorinated Ethene Degradation
journal, January 2002

Anion and Carboxylic Acid Binding to Monotopic and Ditopic Amidopyridine Macrocycles
journal, July 2008

X174 cistron A protein is a multifunctional enzyme in DNA replication
journal, August 1977

Short N-terminal sequences package proteins into bacterial microcompartments
journal, March 2010

Anion Binding to Monotopic and Ditopic Macrocyclic Amides
journal, July 2006

Antimicrobial peptides: premises and promises
journal, December 2004

On the size of the active site in proteases. I. Papain
journal, April 1967

GABAA receptor target of tetramethylenedisulfotetramine
journal, May 2014

Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform
journal, September 2014

Alginate Lyase: Review of Major Sources and Enzyme Characteristics, Structure-Function Analysis, Biological Roles, and Applications
journal, October 2000

Engineering Controllable Protein Degradation
journal, June 2006

Employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in Escherichia coli
journal, July 2016

Identification of common molecular subsequences
journal, March 1981

Prokaryotic nanocompartments form synthetic organelles in a eukaryote
journal, April 2018

A synthetic system for expression of components of a bacterial microcompartment
journal, September 2013

A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method
journal, October 2014

Activation of RegB endoribonuclease by S1 ribosomal protein requires an 11 nt conserved sequence
journal, November 2006

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997

The expanding scope of antimicrobial peptide structures and their modes of action
journal, September 2011

Identification of a Possible Secondary Picrotoxin-Binding Site on the GABA _A Receptor
journal, September 2013

Characterization of De Novo Synthesized GPCRs Supported in Nanolipoprotein Discs
journal, September 2012

Simulations of the BM2 Proton Channel Transmembrane Domain from Influenza Virus B
journal, October 2009

Designer microbes for biosynthesis
journal, October 2014

Design of orthogonal genetic switches based on a crosstalk map of σs, anti‐σs, and promoters
journal, January 2013

Biological Properties of Structurally Related α-Helical Cationic Antimicrobial Peptides
journal, April 1999

Trichloroethylene oxidation by purified toluene 2-monooxygenase: products, kinetics, and turnover-dependent inactivation.
journal, January 1997

The NRF2-KEAP1 Pathway Is an Early Responsive Gene Network in Arsenic Exposed Lymphoblastoid Cells
journal, February 2014

Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin
journal, June 2016

A Role for Loop F in Modulating GABA Binding Affinity in the GABAA Receptor
journal, September 2012