Switchable photosystem-II designer algae for photobiological hydrogen production

Lee, James Weifu

Title: Switchable photosystem-II designer algae for photobiological hydrogen production

Patent · Tue Jan 05 00:00:00 EST 2010

OSTI ID:1015190

Lee, James Weifu ^[1]

Knoxville, TN

A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H.sub.2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF.sub.1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H.sub.2 from the switchable PSII designer alga.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-00OR22725

Assignee:: Lee, James Weifu (Knoxville, TN)

Patent Number(s):: 7,642,405

Application Number:: 11/748,550

OSTI ID:: 1015190

Country of Publication:: United States

Language:: English

References (14)

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