DHAD variants and methods of screening
Abstract
Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
- Inventors:
- Issue Date:
- Research Org.:
- Butamax Advanced Biofuels LLC Wilmington, DE (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1345383
- Patent Number(s):
- 9580705
- Application Number:
- 14/207,823
- Assignee:
- Butamax Advanced Biofuels LLC
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AR0000006
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2014 Mar 13
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Kelly, Kristen J., and Ye, Rick W. DHAD variants and methods of screening. United States: N. p., 2017.
Web.
Kelly, Kristen J., & Ye, Rick W. DHAD variants and methods of screening. United States.
Kelly, Kristen J., and Ye, Rick W. Tue .
"DHAD variants and methods of screening". United States. https://www.osti.gov/servlets/purl/1345383.
@article{osti_1345383,
title = {DHAD variants and methods of screening},
author = {Kelly, Kristen J. and Ye, Rick W.},
abstractNote = {Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 28 00:00:00 EST 2017},
month = {Tue Feb 28 00:00:00 EST 2017}
}
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