Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials

Title: Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials

Abstract

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.

Inventors:: Cascao-Pereira, Luis G; Kaper, Thijs; Kelemen, Bradley R; Liu, Amy D

Issue Date:: 2015-04-07

Research Org.:: Danisco US Inc., Palo Alto, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1177516

Patent Number(s):: 8999692

Application Number:: 13/543,627

Assignee:: Danisco US Inc. (Palo Alto, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/2437 - {Cellulases

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
Y02E50/10 - Biofuels

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/02 - Monosaccharides
C12P19/14 - produced by the action of a carbohydrase {

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y302/01091 - Cellulose 1,4-beta-cellobiosidase
C12Y302/01004 - Cellulase

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DOE Contract Number:: FC36-08GO18078

Resource Type:: Patent

Resource Relation:: Patent File Date: 2012 Jul 06

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Cascao-Pereira, Luis G, Kaper, Thijs, Kelemen, Bradley R, and Liu, Amy D. Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials.  United States: N. p., 2015. 
        Web.

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                    Cascao-Pereira, Luis G, Kaper, Thijs, Kelemen, Bradley R, & Liu, Amy D. Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials.  United States.

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                    Cascao-Pereira, Luis G, Kaper, Thijs, Kelemen, Bradley R, and Liu, Amy D. Tue .  
        "Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials".  United States.  https://www.osti.gov/servlets/purl/1177516.

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@article{osti_1177516,

  title        = {Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials},

  author       = {Cascao-Pereira, Luis G and Kaper, Thijs and Kelemen, Bradley R and Liu, Amy D},

  abstractNote = {The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2015},

  month        = {4}

}

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Similar Records

Title: Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials

Abstract

Citation Formats

Basic local alignment search tool journal, October 1990

Hydrolysis of Cellulose Using Ternary Mixtures of Purified Cellulases book, January 1998

Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes journal, May 1991

Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea journal, January 1996

Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase journal, May 2002

Cellulase families and their genes journal, September 1987

The roles and function of cellulose-binding domains journal, September 1997

Molecular Biology of Cellulolytic Fungi book, January 1995

Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor book, January 2003

Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations journal, July 1984

Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27 journal, October 1983

Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II journal, January 1987

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis journal, January 1988

Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei: Separation of functional domains journal, August 1986

Crystallographic evidence for substrate ring distortion and protein conformational changes during catalysis in cellobiohydrolase Ce16A from Trichoderma reesei journal, September 1999

Molecular cloning, transcriptional, and expression analysis of the first cellulase gene (cbh2), encoding cellobiohydrolase II, from the moderately thermophilic fungus Talaromyces emersonii and structure prediction of the gene product journal, February 2003

Basic local alignment search tool
journal, October 1990

Hydrolysis of Cellulose Using Ternary Mixtures of Purified Cellulases
book, January 1998

Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes
journal, May 1991

Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea
journal, January 1996

Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase
journal, May 2002

Cellulase families and their genes
journal, September 1987

The roles and function of cellulose-binding domains
journal, September 1997

Molecular Biology of Cellulolytic Fungi
book, January 1995

Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor
book, January 2003

Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations
journal, July 1984

Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27
journal, October 1983

Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II
journal, January 1987

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis
journal, January 1988

Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei: Separation of functional domains
journal, August 1986

Crystallographic evidence for substrate ring distortion and protein conformational changes during catalysis in cellobiohydrolase Ce16A from Trichoderma reesei
journal, September 1999

Molecular cloning, transcriptional, and expression analysis of the first cellulase gene (cbh2), encoding cellobiohydrolase II, from the moderately thermophilic fungus Talaromyces emersonii and structure prediction of the gene product
journal, February 2003