U.S. Department of EnergyOffice of Scientific and Technical Information
DHAD variants and methods of screening
Abstract
Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
- Inventors:
- Publication Date:
- Research Org.:
- Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568323
- Patent Number(s):
- 10,287,566
- Application Number:
- 15/406,876
- Assignee:
- Butamax Advanced Biofuels LLC (Wilmington, DE)
- DOE Contract Number:
- AR0000006
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 01/16/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Kelly, Kristen J., and Ye, Rick W. DHAD variants and methods of screening. United States: N. p., 2019.
Web.
Kelly, Kristen J., & Ye, Rick W. DHAD variants and methods of screening. United States.
Kelly, Kristen J., and Ye, Rick W. 2019.
"DHAD variants and methods of screening". United States. https://www.osti.gov/servlets/purl/1568323.
@article{osti_1568323,
title = {DHAD variants and methods of screening},
author = {Kelly, Kristen J. and Ye, Rick W.},
abstractNote = {Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.},
doi = {},
url = {https://www.osti.gov/biblio/1568323},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 14 00:00:00 EDT 2019},
month = {Tue May 14 00:00:00 EDT 2019}
}
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