DHAD variants and methods of screening
Abstract
Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
- Inventors:
- Issue Date:
- Research Org.:
- Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568323
- Patent Number(s):
- 10287566
- Application Number:
- 15/406,876
- Assignee:
- Butamax Advanced Biofuels LLC (Wilmington, DE)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AR0000006
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 01/16/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Kelly, Kristen J., and Ye, Rick W. DHAD variants and methods of screening. United States: N. p., 2019.
Web.
Kelly, Kristen J., & Ye, Rick W. DHAD variants and methods of screening. United States.
Kelly, Kristen J., and Ye, Rick W. Tue .
"DHAD variants and methods of screening". United States. https://www.osti.gov/servlets/purl/1568323.
@article{osti_1568323,
title = {DHAD variants and methods of screening},
author = {Kelly, Kristen J. and Ye, Rick W.},
abstractNote = {Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {5}
}
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Works referenced in this record:
Nucleic acid coding for an .alpha.-acetolactate synthase from lactococcus and its applications
patent, July 1997
- Ehrlich, Stanislav; Godon, Jean-Jacques; Renault, Pierre
- US Patent Document 5,643,779
Fermentive production of four carbon alcohols
patent, September 2014
- Donaldson, Gail K.; Eliot, Andrew C.; Maggio-Hall, Lori Ann
- US Patent Document 8,828,704
Fermentive production of four carbon alcohols
patent, March 2015
- Donaldson, Gail K.; Eliot, Andrew C.; Nagarajan, Vasantha
- US Patent Document 8,980,612
Isobutanol Tolerance in Yeast with an Altered Lipid Profile
patent-application, November 2016
- Van Dyk, Tina K.; Xue, Zhixiong; Yadav, Narendra S.
- US Patent Application 15/108845; 20160326551
Fermentive production of four carbon alcohols
patent, November 2014
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,889,385
Nucleic acid and amino acid sequences relating to Streptococcus pneumoniae for diagnostics and therapeutics
patent, March 2004
- Doucette-Stamm, Lynn; Bush, David; Zeng, Qiandong
- US Patent Document 6,699,703
Fermentive production of four carbon alcohols
patent, September 2012
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,273,558
Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
patent, June 2013
- Paul, Brian James
- US Patent Document 8,455,224
Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway
patent, June 2013
- Bramucci, Michael G.; Larossa, Robert A.; Smulski, Dana R.
- US Patent Document 8,455,225
Fermentive production of four carbon alcohols
patent, May 2014
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,735,114
Bacterial Strains for Butanol Production
patent-application, August 2011
- Euler, Lori Jean; Flint, Dennis; Paul, Brian James
- US Patent Application 12/899760; 20110195505
Solvent tolerant microorganisms and methods of isolation
patent, February 2010
- Bramucci, Michael G.; Nagarajan, Vasantha; Sedkova, Natalia
- US Patent Document 7,659,104
Yeast with increased butanol tolerance involving a multidrug efflux pump gene
patent, December 2013
- Van Dyk, Tina K.
- US Patent Document 8,614,085
Fermentive production of four carbon alcohols
patent, June 2016
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 9,365,872
Fermentative Production of Alcohols
patent-application, August 2015
- Bhadra, Bhaskar; Huang, Lixuan Lisa
- US Patent Application 14/422282; 20150218595
Fermentive production of four carbon alcohols
patent, May 2012
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,178,328
Flux to acetolactate-derived products in lactic acid bacteria
patent, October 2015
- Paul, Brian James; Suh, Wonchul
- US Patent Document 9,169,499
Ketol-acid reductoisomerase using NADH
patent, January 2016
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 9,238,801
Host cells and methods for production of isobutanol
patent, August 2016
- Anthony, Larry Cameron; He, Hongxian; Huang, Lixuan Lisa
- US Patent Document 9,422,582
Engineering Resistance to Aliphatic Alcohols
patent-application, March 2012
- Alsaker, Keith; Grenfell-Lee, Daniel; Hudson, Michael
- US Patent Application 13/214310; 20120058541
Ketol-acid reductoisomerase using NADH
patent, November 2014
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 8,895,307
Cytosolic Isobutanol Pathway Localization for the Production of Isobutanol
patent-application, March 2011
- Urano, Jun; Dundon, Catherine Asleson; Meinhold, Peter
- US Patent Application 12/855276; 20110076733
Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols
patent, July 2014
- Satagopan, Sriram; O'Keefe, Daniel P.; Gude, Janardhan
- US Patent Document 8,765,433
Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
patent, May 2012
- Bramucci, Michael G.; Eliot, Andrew C.; Maggio-Hall, Lori Ann
- US Patent Document 8,188,250
Fermentive production of four carbon alcohols
patent, December 2010
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 7,851,188
Lignocellulosic Hydrolysates as Feedstocks for Isobutanol Fermentation
patent-application, February 2013
- Dobson, Ian David; Kruckeberg, Arthur Leo
- US Patent Application 13/525032; 20130035515
Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
patent, August 2016
- Anthony, Larry Cameron
- US Patent Document 9,404,117
Dhad Variants for Butanol Production
patent-application, July 2014
- Maggio-Hall, Loriann Ann; Paul, Brian James; Rothman, Steven Cary
- US Patent Application 14/142,398; 2014/0186910 Al
Process for amplifying nucleic acid sequences
patent, July 1987
- Mullis, Kary B.
- US Patent Document 4,683,202
Method for producing butanol using extractive fermentation with electrolyte addition
patent, December 2013
- Grady, Michael Charles; Patnaik, Ranjan
- US Patent Document 8,617,861
Engineering resistance to aliphatic alcohols
patent, December 2014
- Alsaker, Keith; Grenfell-Lee, Daniel; Hudson, Michael
- US Patent Document 8,906,666
Identification and use of Bacterial [2FE-2S] Dihydroxy-acid Dehydratases
patent-application, April 2010
- Flint, Dennis; Rothman, Steven Cary; Suh, Wonchul
- US Patent Application 12/569636; 20100081154
Activity of FE-S Cluster Requiring Proteins
patent-application, February 2014
- Flint, Dennis; Paul, Brian James; Ye, Rick W.
- US Patent Application 13/837921; 20140038263
Genetic switches for butanol production
patent, November 2015
- Dauner, Michael; Kruckeberg, Arthur Leo; Larossa, Robert A.
- US Patent Document 9,181,566
Yeast production host cells
patent, February 2016
- Anthony, Larry Cameron; Nelson, Mark J.
- US Patent Document 9,260,708
Fermentive production of four carbon alcohols
patent, October 2012
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,283,144
Keto-isovalerate decarboxylase enzymes and methods of use thereof
patent, January 2016
- McElvain, Jessica; O'Keefe, Daniel P.; Paul, Brian James
- US Patent Document 9,238,828
Deletion mutants for the production of isobutanol
patent-application, December 2009
- Donaldson, Gail K.; Maggio-Hall, Lori Ann; Nakamura, Charles E.
- US Patent Application 12/477946; 20090305369
Method for the detection of melamine
patent, January 2014
- Krebs, Joseph
- US Patent Document 8,637,269
Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase
patent, June 2012
- Eliot, Andrew C.; Nagarajan, Vasantha; Huang, Lixuan Lisa
- US Patent Document 8,206,970
Enhanced pyruvate to acetolactate conversion in yeast
patent, March 2014
- Anthony, Larry Cameron; Maggio-Hall, Lori Ann
- US Patent Document 8,669,094
Strain for butanol production with increased membrane unsaturated trans fatty acids
patent, February 2014
- Flint, Dennis; Van Dyk, Tina K.
- US Patent Document 8,652,823
Fermentive Production of Isobutanol Using Highly Effective Ketol-acid Reductoisomerase Enzymes
patent-application, October 2011
- Nagarajan, Vasantha; Paul, Brian James; Suh, Wonchul
- US Patent Application 12/893077; 20110244536
Method for the fermentative production of D-pantothenic acid using Coryneform bacteria
patent, January 2001
- Eggeling, Lothar; Thierbach, Georg; Sahm, Hermann
- US Patent Document 6,177,264
Acetate Supplementation of Medium for Butanologens
patent-application, September 2013
- Maggio-Hall, Lori Ann
- US Patent Application 13/833369; 20130252296
Strain comprising increased expression of a CFA coding region for butanol production
patent, August 2013
- Flint, Dennis; Larossa, Robert A.; Nagarajan, Vasantha
- US Patent Document 8,518,678
Treatment of biomass to obtain fermentable sugars
patent-application, February 2007
- Dunson, Jr., James B.; Elander, Richard T.; Tucker, III, Melvin P.
- US Patent Application 11/402757; 20070031918
Production of isobutanol by a microorganism with enhanced acetolactate synthase activity
patent, February 2015
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,945,859
Enhanced pyruvate to acetolactate conversion in yeast
patent, February 2015
- Anthony, Larry Cameron; Maggio-Hall, Lori Ann
- US Patent Document 8,956,850
Fermentive production of four carbon alcohols
patent, March 2016
- Donaldson, Gail K.; Huang, Lixuan Lisa; Maggio-Hall, Lori Ann
- US Patent Document 9,297,028
Fermentive production of four carbon alcohols
patent, March 2016
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 9,297,029
Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
patent, January 2014
- Paul, Brian James; Suh, Wonchul
- US Patent Document 8,637,281
Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
patent, April 2014
- Bramucci, Michael G.; Eliot, Andrew C.; Maggio-Hall, Lori Ann
- US Patent Document 8,691,540
Carbon Pathway Optimized Production Hosts for the Production of Isobutanol
patent-application, May 2010
- Anthony, Larry Cameron; Dauner, Michael; Donaldson, Gail K.
- US Patent Application 12/606410; 20100120105
Ketol-acid reductoisomerase using NADH
patent, December 2014
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 8,911,981
Organic Acid Production by Fungal Cells
patent-application, February 2011
- Winkler, Aaron Adriaan; De Hulster, Abraham Frederik; Van Dijken, Johannes Pieter
- US Patent Application 12/600559; 20110039327
Host Cells and Methods for Production of Isobutanol
patent-application, January 2014
- Anthony, Larry Cameron; He, Hongxian; Huang, Lixuan Lisa
- US Patent Application 13/840676; 20140030783
Recombinant host cell comprising a diol dehydratase
patent, February 2015
- Donaldson, Gail K.; Eliot, Andrew C.; Nagarajan, Vasantha
- US Patent Document 8,962,298
Ketol-acid reductoisomerase using NADH
patent, July 2012
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 8,222,017
Method for the production of isobutanol by recombinant yeast
patent, April 2016
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 9,303,225
Fermentive production of four carbon alcohols
patent, February 2015
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,951,774
Solvent tolerant microorganisms and methods of isolation
patent, June 2009
- Bramucci, Michael G.; Nagarajan, Vasantha; Sedkova, Natalia
- US Patent Document 7,541,173
Activity of Fe—S cluster requiring proteins
patent, March 2016
- Flint, Dennis; Paul, Brian James; Ye, Rick W.
- US Patent Document 9,297,016
Yeast with increased butanol tolerance involving filamentous growth response
patent, October 2013
- Bramucci, Michael G.; Larossa, Robert A.; Singh, Manjari
- US Patent Document 8,557,562
Yeast strain for production of four carbon alcohols
patent, January 2015
- Larossa, Robert A.
- US Patent Document 8,940,511
Fermentive Production of Four Carbon Alcohols
patent-application, August 2012
- Donaldson, Gail K.; Eliot, Andrew C.; Nagarajan, Vasantha
- US Patent Application 13/281872; 20120196341
Cytosolic isobutanol pathway localization for the production of isobutanol
patent, July 2012
- Urano, Jun; Dundon, Catherine Asleson
- US Patent Document 8,232,089
Recombinant yeast host cells comprising FE-S cluster proteins
patent, December 2015
- Anthony, Larry Cameron; Rothman, Steven Cary; Tomb, Jean-Francois
- US Patent Document 9,206,447
Method for producing butanol using extractive fermentation with electrolyte addition
patent, March 2015
- Grady, Michael Charles; Patnaik, Ranjan
- US Patent Document 8,969,055
Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases
patent, February 2015
- Flint, Dennis; Rothman, Steven Cary; Suh, Wonchul
- US Patent Document 8,951,937
Recombinant host cells comprising phosphoketolases
patent, October 2014
- Dauner, Michael; Maggio-Hall, Lori Ann; Tomb, Jean-Francois
- US Patent Document 8,871,488
Fermentive production of four carbon alcohols
patent, June 2015
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 9,068,190
Ketol-acid reductoisomerase enzymes and methods of use
patent, October 2015
- Govindarajan, Sridhar; Li, Yougen; Liao, Der-Ing
- US Patent Document 9,169,467
Butanol tolerance in microorganisms
patent, March 2016
- Bramucci, Michael G.; Nagarajan, Vasantha
- US Patent Document 9,273,330
Production of isobutanol in yeast mitochondria
patent, September 2014
- Anthony, Larry Cameron; Huang, Lixuan Lisa; Ye, Rick W.
- US Patent Document 8,828,694
Fermentive production of four carbon alcohols
patent, August 2011
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 7,993,889
Method for producing butanol using two-phase extractive fermentation
patent, September 2014
- Grady, Michael Charles; Jahic, Mehmedalija; Patnaik, Ranjan
- US Patent Document 8,828,695
Yeast strain for production of four carbon alcohols
patent, March 2013
- Larossa, Robert A.
- US Patent Document 8,389,252
Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
patent, March 2016
- Liao, Der-Ing; Nelson, Mark J.; Bramucci, Michael G.
- US Patent Document 9,284,612
Ketol-acid reductoisomerase enzymes and methods of use
patent, July 2016
- Govindarajan, Sridhar; Li, Yougen; Liao, Der-Ing
- US Patent Document 9,388,392
Enhanced pyruvate to acetolactate conversion in yeast
patent, March 2015
- Anthony, Larry Cameron; Maggio-Hall, Lori Ann
- US Patent Document 8,969,065
Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
patent, June 2013
- Anthony, Larry Cameron
- US Patent Document 8,465,964
Treatment of biomass to obtain fermentable sugars
patent, April 2011
- Dunson, Jr., James B.; Tucker, Melvin P.; Elander, Richard T.
- US Patent Document 7,932,063
Yeast with increased butanol tolerance involving cell wall integrity pathway
patent, August 2014
- Bramucci, Michael G.; Larossa, Robert A.; Smulski, Dana R.
- US Patent Document 8,795,992
Ketol-acid reductoisomerase using NADH
patent, March 2012
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 8,129,162
Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
patent, September 2011
- Dundon, Catherine Asleson; Aristidou, Aristos; Hawkins, Andrew
- US Patent Document 8,017,376
Production of four carbon alcohols using improved strain
patent, February 2013
- Larossa, Robert A.; Smulski, Dana R.; Nagarajan, Vasantha
- US Patent Document 8,372,612
Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
patent, September 2012
- Dundon, Catherine Asleson; Aristidou, Aristos; Hawkins, Andrew
- US Patent Document 8,273,565
Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
patent, July 2014
- Anthony, Larry Cameron
- US Patent Document 8,785,166
Butanol strain improvement with integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
patent, February 2016
- Anthony, Larry Cameron; Maggio-Hall, Lori Ann
- US Patent Document 9,267,157
Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
patent, October 2015
- Anthony, Larry Cameron
- US Patent Document 9,163,266
Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
patent, July 2015
- Paul, Brian James
- US Patent Document 9,080,179
Enhanced Iron-sulfur Cluster Formation for Increased Dihydroxy-Acid Dehydratase Activity in Lactic Acid Bacteria
patent-application, April 2010
- Paul, Brain James; Suh, Wonchul
- US Patent Application 12/569103; 20100081182
Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
patent, March 2011
- Liao, Der-Ing; Nelson, Mark J.; Bramucci, Michael G.
- US Patent Document 7,910,342
Ketol-acid reductoisomerase using NADH
patent, February 2015
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 8,945,899
Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof
patent, August 2012
- Anthony, Larry Cameron; Flint, Dennis; Suh, Wonchul
- US Patent Document 8,241,878
Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
patent, December 2011
- Dundon, Catherine Asleson; Aristidou, Aristos; Hawkins, Andrew
- US Patent Document 8,071,358
Host Cells and Methods for Production of Isobutanol
patent-application, January 2014
- Anthony, Larry Cameron; He, Hongxian; Huang, Lixuan Lisa
- US Patent Application 13/840649; 20140030782
Solvent tolerant microorganisms
patent, September 2011
- Bramucci, Michael G.; Kane, Helene M. A.; Nagarajan, Vasantha
- US Patent Document 8,017,364