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Title: DHAD variants and methods of screening

Abstract

Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

Inventors:
;
Issue Date:
Research Org.:
Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1568323
Patent Number(s):
10287566
Application Number:
15/406,876
Assignee:
Butamax Advanced Biofuels LLC (Wilmington, DE)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
DOE Contract Number:  
AR0000006
Resource Type:
Patent
Resource Relation:
Patent File Date: 01/16/2017
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Kelly, Kristen J., and Ye, Rick W. DHAD variants and methods of screening. United States: N. p., 2019. Web.
Kelly, Kristen J., & Ye, Rick W. DHAD variants and methods of screening. United States.
Kelly, Kristen J., and Ye, Rick W. Tue . "DHAD variants and methods of screening". United States. https://www.osti.gov/servlets/purl/1568323.
@article{osti_1568323,
title = {DHAD variants and methods of screening},
author = {Kelly, Kristen J. and Ye, Rick W.},
abstractNote = {Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {5}
}

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