RNA binding protein and binding site useful for expression of recombinant molecules
Abstract
The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.
- Inventors:
-
- Cardiff, CA
- Issue Date:
- Research Org.:
- Scripps Research Institute (La Jolla, CA)
- OSTI Identifier:
- 873426
- Patent Number(s):
- 6156517
- Assignee:
- Scripps Research Institute (La Jolla, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-93ER20116
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- rna; binding; protein; site; useful; expression; recombinant; molecules; relates; eukaryotic; prokaryotic; cells; preferably; plant; intact; plants; particular; rb47; upstream; translation; initiation; regulation; mediated; poly; family; effected; rb60; disulfide; isomerase; capable; functioning; nuclear; cytoplasm; chloroplast; desired; molecule; enhanced; approximately; 100; fold; obtained; activation; binding site; binding protein; plant cell; plant cells; recombinant molecule; site useful; /435/999/
Citation Formats
Mayfield, Stephen. RNA binding protein and binding site useful for expression of recombinant molecules. United States: N. p., 2000.
Web.
Mayfield, Stephen. RNA binding protein and binding site useful for expression of recombinant molecules. United States.
Mayfield, Stephen. Sat .
"RNA binding protein and binding site useful for expression of recombinant molecules". United States. https://www.osti.gov/servlets/purl/873426.
@article{osti_873426,
title = {RNA binding protein and binding site useful for expression of recombinant molecules},
author = {Mayfield, Stephen},
abstractNote = {The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
Works referenced in this record:
Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins.
journal, December 1991
- Danon, A.; Mayfield, S. P.
- The EMBO Journal, Vol. 10, Issue 13