Cloning and expression of recombinant adhesive protein MEFP-2 of the blue mussel, Mytilus edulis
Abstract
The present invention includes a Mytilus edulis cDNA having a nucleotide sequence that encodes for the Mytilus edulis foot protein-2 (Mefp-2), an example of a mollusk foot protein. Mefp-2 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp-2 gene will allow researchers to produce Mefp-2 protein using genetic engineering techniques. The discovery of Mefp-2 gene sequences will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.
- Inventors:
- Issue Date:
- Research Org.:
- Idaho National Laboratory (INL), Idaho Falls, ID (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175639
- Patent Number(s):
- 6995012
- Application Number:
- 10/915,160
- Assignee:
- Battelle Energy Alliance, LLC (Idaho Falls, ID)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- AC07-05ID14517
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Silverman, Heather G., and Roberto, Francisco F. Cloning and expression of recombinant adhesive protein MEFP-2 of the blue mussel, Mytilus edulis. United States: N. p., 2006.
Web.
Silverman, Heather G., & Roberto, Francisco F. Cloning and expression of recombinant adhesive protein MEFP-2 of the blue mussel, Mytilus edulis. United States.
Silverman, Heather G., and Roberto, Francisco F. Tue .
"Cloning and expression of recombinant adhesive protein MEFP-2 of the blue mussel, Mytilus edulis". United States. https://www.osti.gov/servlets/purl/1175639.
@article{osti_1175639,
title = {Cloning and expression of recombinant adhesive protein MEFP-2 of the blue mussel, Mytilus edulis},
author = {Silverman, Heather G. and Roberto, Francisco F.},
abstractNote = {The present invention includes a Mytilus edulis cDNA having a nucleotide sequence that encodes for the Mytilus edulis foot protein-2 (Mefp-2), an example of a mollusk foot protein. Mefp-2 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp-2 gene will allow researchers to produce Mefp-2 protein using genetic engineering techniques. The discovery of Mefp-2 gene sequences will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {2}
}
Works referenced in this record:
Adhesion and substrate choice in mussels and barnacles
journal, March 1985
- Crisp, D. J.; Walker, G.; Young, G. A.
- Journal of Colloid and Interface Science, Vol. 104, Issue 1
Role of l -3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins
journal, June 1999
- Yu, Miaoer; Hwang, Jungyeon; Deming, Timothy J.
- Journal of the American Chemical Society, Vol. 121, Issue 24
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997
- Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A.
- Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402
The Adhesive Protein cDNA of Mytilus galloprovincialis Encodes Decapeptide Repeats but No Hexapeptide Motif
journal, June 1994
- Inoue, K.; Odo, S.
- The Biological Bulletin, Vol. 186, Issue 3
Expression Sites of Two Byssal Protein Genes of Mytilus galloprovincialis
journal, April 1996
- Miki, D.; Takeuchi, Y.; Inoue, K.
- The Biological Bulletin, Vol. 190, Issue 2
Minireview-Polyphenols and Oxidases in Substratum Adhesion by Marine Algae and Mussels
journal, February 1998
- Vreeland, Valerie; Waite, J. Herbert; Epstein, Lynn
- Journal of Phycology, Vol. 34, Issue 1
Mussel Adhesive Plaque Protein Gene Is a Novel Member of Epidermal Growth Factor-like Gene Family
journal, March 1995
- Inoue, Koji; Takeuchi, Yasuhiro; Miki, Daisuke
- Journal of Biological Chemistry, Vol. 270, Issue 12
Metal-Mediated Cross-Linking in the Generation of a Marine-Mussel Adhesive
journal, January 2004
- Sever, Mary J.; Weisser, Jaime T.; Monahan, Jennifer
- Angewandte Chemie International Edition, Vol. 43, Issue 4
A potential mediator of collagenous block copolymer gradients in mussel byssal threads
journal, September 1998
- Qin, X. -X.; Waite, J. H.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 18
Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993
- Karlin, S.; Altschul, S. F.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877
Characterization of a Cystine-Rich Polyphenolic Protein Family from the Blue Mussel Mytilus edulis L
journal, August 1992
- Rzepecki, L. M.; Hansen, K. M.; Waite, J. H.
- The Biological Bulletin, Vol. 183, Issue 1
Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline
journal, May 1981
- Waite, J. H.; Tanzer, M. L.
- Science, Vol. 212, Issue 4498
Polyphosphoprotein from the Adhesive Pads of Mytilus edulis †
journal, March 2001
- Waite, J. Herbert; Qin, Xiaoxia
- Biochemistry, Vol. 40, Issue 9
Hydroxyarginine-containing Polyphenolic Proteins in the Adhesive Plaques of the Marine Mussel Mytilus edulis
journal, August 1995
- Papov, Vladimir V.; Diamond, Thomas V.; Biemann, Klaus
- Journal of Biological Chemistry, Vol. 270, Issue 34
Mussel Adhesive Enhances the Immobilization of Human Chorionic Gonadotrophin to a Solid Support
journal, October 1996
- Burzio, Verónica A.; Silva, Tatiana; Pardo, Joel
- Analytical Biochemistry, Vol. 241, Issue 2