Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis

Silverman, Heather G.; Roberto, Francisco F.

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Title: Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis

Abstract

The present invention comprises a Mytilus edulis cDNA sequenc having a nucleotide sequence that encodes for the Mytilus edulis foot protein-1 (Mefp-1), an example of a mollusk foot protein. Mefp-1 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp-1 gene will allow researchers to produce Mefp-1 protein using genetic engineering techniques. The discovery of Mefp-1 gene sequence will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.

Inventors:: Silverman, Heather G.; Roberto, Francisco F.

Issue Date:: 2006-01-17

Research Org.:: Idaho National Laboratory (INL), Idaho Falls, ID (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1175628

Patent Number(s):: 6987170

Application Number:: 10/915,161

Assignee:: Battelle Energy Alliance, LLC (Idaho Falls, ID)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/43504 - {from invertebrates}
C07K5/1013 - {the side chain containing O or S as heteroatoms, e.g. Cys, Ser}

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DOE Contract Number:: AC07-99JD13727

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES

Citation Formats


                    Silverman, Heather G., and Roberto, Francisco F. Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis.  United States: N. p., 2006. 
        Web.

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                    Silverman, Heather G., & Roberto, Francisco F. Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis.  United States.

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                    Silverman, Heather G., and Roberto, Francisco F. Tue .  
        "Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis".  United States.  https://www.osti.gov/servlets/purl/1175628.

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@article{osti_1175628,

  title        = {Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis},

  author       = {Silverman, Heather G. and Roberto, Francisco F.},

  abstractNote = {The present invention comprises a Mytilus edulis cDNA sequenc having a nucleotide sequence that encodes for the Mytilus edulis foot protein-1 (Mefp-1), an example of a mollusk foot protein. Mefp-1 is an integral component of the blue mussels' adhesive protein complex, which allows the mussel to attach to objects underwater. The isolation, purification and sequencing of the Mefp-1 gene will allow researchers to produce Mefp-1 protein using genetic engineering techniques. The discovery of Mefp-1 gene sequence will also allow scientists to better understand how the blue mussel creates its waterproof adhesive protein complex.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2006},

  month        = {1}

}

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Works referenced in this record:

Adhesion and substrate choice in mussels and barnacles
journal, March 1985

Crisp, D. J.; Walker, G.; Young, G. A.
Journal of Colloid and Interface Science, Vol. 104, Issue 1
https://doi.org/10.1016/0021-9797(85)90007-4

The Adhesive Protein cDNA of Mytilus galloprovincialis Encodes Decapeptide Repeats but No Hexapeptide Motif
journal, June 1994

Inoue, K.; Odo, S.
The Biological Bulletin, Vol. 186, Issue 3
https://doi.org/10.2307/1542281

Expression Sites of Two Byssal Protein Genes of Mytilus galloprovincialis
journal, April 1996

Miki, D.; Takeuchi, Y.; Inoue, K.
The Biological Bulletin, Vol. 190, Issue 2
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Preliminary Evaluation of the Use of Mussel Adhesive Protein in Experimental Epikeratoplasty
journal, July 1988

Robin, J. B.; Picciano, P.; Kusleika, R. S.
Archives of Ophthalmology, Vol. 106, Issue 7
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Mussel glue fromMytilus californianus Conrad: a comparative study
journal, January 1986

Waite, J. Herbert
Journal of Comparative Physiology B, Vol. 156, Issue 4
https://doi.org/10.1007/BF00691034

Mussel Adhesive Plaque Protein Gene Is a Novel Member of Epidermal Growth Factor-like Gene Family
journal, March 1995

Inoue, Koji; Takeuchi, Yasuhiro; Miki, Daisuke
Journal of Biological Chemistry, Vol. 270, Issue 12
https://doi.org/10.1074/jbc.270.12.6698

Metal-Mediated Cross-Linking in the Generation of a Marine-Mussel Adhesive
journal, January 2004

Sever, Mary J.; Weisser, Jaime T.; Monahan, Jennifer
Angewandte Chemie International Edition, Vol. 43, Issue 4
https://doi.org/10.1002/anie.200352759

Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes.
journal, March 1990

Karlin, S.; Altschul, S. F.
Proceedings of the National Academy of Sciences, Vol. 87, Issue 6
https://doi.org/10.1073/pnas.87.6.2264

Characterization of a Cystine-Rich Polyphenolic Protein Family from the Blue Mussel Mytilus edulis L
journal, August 1992

Rzepecki, L. M.; Hansen, K. M.; Waite, J. H.
The Biological Bulletin, Vol. 183, Issue 1
https://doi.org/10.2307/1542413

Mussel Adhesive Enhances the Immobilization of Human Chorionic Gonadotrophin to a Solid Support
journal, October 1996

Burzio, Verónica A.; Silva, Tatiana; Pardo, Joel
Analytical Biochemistry, Vol. 241, Issue 2
https://doi.org/10.1006/abio.1996.0398

Role of l -3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins
journal, June 1999

Yu, Miaoer; Hwang, Jungyeon; Deming, Timothy J.
Journal of the American Chemical Society, Vol. 121, Issue 24
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Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997

Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A.
Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402
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Attachment of epiphyseal cartilage cells and 17/28 rat osteosarcoma osteoblasts using mussel adhesive protein
journal, November 1990

Fulkerson, John P.; Norton, Louis A.; Gronowicz, Gloria
Journal of Orthopaedic Research, Vol. 8, Issue 6
https://doi.org/10.1002/jor.1100080603

Minireview-Polyphenols and Oxidases in Substratum Adhesion by Marine Algae and Mussels
journal, February 1998

Vreeland, Valerie; Waite, J. Herbert; Epstein, Lynn
Journal of Phycology, Vol. 34, Issue 1
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Expression of multiple forms of an adhesive plaque protein in an individual mussel, Mytilus edulis
journal, September 1999

Warner, S. C.; Waite, J. H.
Marine Biology, Vol. 134, Issue 4
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Cloning, Sequencing and Sites of Expression of Genes for the Hydroxyarginine-Containing Adhesive-Plaque Protein of the Mussel Mytilus galloprovincialis
journal, July 1996

Inoue, Koji; Takeuchi, Yasuhiro; Miki, Daisuke
European Journal of Biochemistry, Vol. 239, Issue 1
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Reverse Engineering of Bioadhesion in Marine Mussels
journal, June 1999

Waiter, J. Herbert
Annals of the New York Academy of Sciences, Vol. 875, Issue 1
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A potential mediator of collagenous block copolymer gradients in mussel byssal threads
journal, September 1998

Qin, X. -X.; Waite, J. H.
Proceedings of the National Academy of Sciences, Vol. 95, Issue 18
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Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993

Karlin, S.; Altschul, S. F.
Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877
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Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline
journal, May 1981

Waite, J. H.; Tanzer, M. L.
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The strength of adhesive-bonded tissue joints
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The phylogeny and chemical diversity of quinone-tanned glues and varnishes
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Biochemistry, Vol. 40, Issue 9
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Hydroxyarginine-containing Polyphenolic Proteins in the Adhesive Plaques of the Marine Mussel Mytilus edulis
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Similar Records

Title: Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis

Abstract

Citation Formats

Adhesion and substrate choice in mussels and barnacles journal, March 1985

The Adhesive Protein cDNA of Mytilus galloprovincialis Encodes Decapeptide Repeats but No Hexapeptide Motif journal, June 1994

Expression Sites of Two Byssal Protein Genes of Mytilus galloprovincialis journal, April 1996

Preliminary Evaluation of the Use of Mussel Adhesive Protein in Experimental Epikeratoplasty journal, July 1988

Mussel glue fromMytilus californianus Conrad: a comparative study journal, January 1986

Mussel Adhesive Plaque Protein Gene Is a Novel Member of Epidermal Growth Factor-like Gene Family journal, March 1995

Metal-Mediated Cross-Linking in the Generation of a Marine-Mussel Adhesive journal, January 2004

Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes. journal, March 1990

Characterization of a Cystine-Rich Polyphenolic Protein Family from the Blue Mussel Mytilus edulis L journal, August 1992

Mussel Adhesive Enhances the Immobilization of Human Chorionic Gonadotrophin to a Solid Support journal, October 1996

Role of l -3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins journal, June 1999

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs journal, September 1997

Attachment of epiphyseal cartilage cells and 17/28 rat osteosarcoma osteoblasts using mussel adhesive protein journal, November 1990

Minireview-Polyphenols and Oxidases in Substratum Adhesion by Marine Algae and Mussels journal, February 1998

Expression of multiple forms of an adhesive plaque protein in an individual mussel, Mytilus edulis journal, September 1999

Cloning, Sequencing and Sites of Expression of Genes for the Hydroxyarginine-Containing Adhesive-Plaque Protein of the Mussel Mytilus galloprovincialis journal, July 1996

Reverse Engineering of Bioadhesion in Marine Mussels journal, June 1999

A potential mediator of collagenous block copolymer gradients in mussel byssal threads journal, September 1998

Applications and statistics for multiple high-scoring segments in molecular sequences. journal, June 1993

Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline journal, May 1981

The strength of adhesive-bonded tissue joints journal, May 1997

The phylogeny and chemical diversity of quinone-tanned glues and varnishes journal, January 1990

Polyphosphoprotein from the Adhesive Pads of Mytilus edulis † journal, March 2001

Hydroxyarginine-containing Polyphenolic Proteins in the Adhesive Plaques of the Marine Mussel Mytilus edulis journal, August 1995

Adhesion and substrate choice in mussels and barnacles
journal, March 1985

The Adhesive Protein cDNA of Mytilus galloprovincialis Encodes Decapeptide Repeats but No Hexapeptide Motif
journal, June 1994

Expression Sites of Two Byssal Protein Genes of Mytilus galloprovincialis
journal, April 1996

Preliminary Evaluation of the Use of Mussel Adhesive Protein in Experimental Epikeratoplasty
journal, July 1988

Mussel glue fromMytilus californianus Conrad: a comparative study
journal, January 1986

Mussel Adhesive Plaque Protein Gene Is a Novel Member of Epidermal Growth Factor-like Gene Family
journal, March 1995

Metal-Mediated Cross-Linking in the Generation of a Marine-Mussel Adhesive
journal, January 2004

Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes.
journal, March 1990

Characterization of a Cystine-Rich Polyphenolic Protein Family from the Blue Mussel Mytilus edulis L
journal, August 1992

Mussel Adhesive Enhances the Immobilization of Human Chorionic Gonadotrophin to a Solid Support
journal, October 1996

Role of l -3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins
journal, June 1999

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997

Attachment of epiphyseal cartilage cells and 17/28 rat osteosarcoma osteoblasts using mussel adhesive protein
journal, November 1990

Minireview-Polyphenols and Oxidases in Substratum Adhesion by Marine Algae and Mussels
journal, February 1998

Expression of multiple forms of an adhesive plaque protein in an individual mussel, Mytilus edulis
journal, September 1999

Cloning, Sequencing and Sites of Expression of Genes for the Hydroxyarginine-Containing Adhesive-Plaque Protein of the Mussel Mytilus galloprovincialis
journal, July 1996

Reverse Engineering of Bioadhesion in Marine Mussels
journal, June 1999

A potential mediator of collagenous block copolymer gradients in mussel byssal threads
journal, September 1998

Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993

Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline
journal, May 1981

The strength of adhesive-bonded tissue joints
journal, May 1997

The phylogeny and chemical diversity of quinone-tanned glues and varnishes
journal, January 1990

Polyphosphoprotein from the Adhesive Pads of Mytilus edulis ^†
journal, March 2001

Hydroxyarginine-containing Polyphenolic Proteins in the Adhesive Plaques of the Marine Mussel Mytilus edulis
journal, August 1995