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Title: Large scale DNA microsequencing device

Abstract

A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.

Inventors:
 [1]
  1. Oak Ridge, TN
Issue Date:
Research Org.:
LOCKHEED MARTIN ENRGY SYST INC
OSTI Identifier:
871114
Patent Number(s):
5661028
Assignee:
Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN)
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
scale; dna; microsequencing; device; microminiature; sequencing; apparatus; method; provide; means; simultaneously; obtaining; sequences; plural; polynucleotide; strands; comprises; microchip; channels; etched; standard; lithographic; procedures; chemical; wet; etching; reaction; separating; section; enclosing; accomplished; bonding; transparent; cover; plate; oligonucleotide; strand; chemically; affixed; alkyl; chain; subsequent; nucleotides; selected; complementary; base; pair; target; nucleotide; produce; family; labelled; channel; separated; following; separation; determined; appropriate; detection; oligonucleotide strand; alkyl chain; chemical wet; transparent cover; detection means; apparatus comprises; cover plate; method provide; wet etching; provide means; target nucleotide; apparatus comprise; complementary base; sequencing apparatus; base pair; scale dna; simultaneously obtaining; nucleotide strand; nucleotide strands; microsequencing device; microminiature sequencing; obtaining sequences; polynucleotide strands; plural polynucleotide; plural channels; dna microsequencing; lithographic procedures; /435/204/216/423/999/

Citation Formats

Foote, Robert S. Large scale DNA microsequencing device. United States: N. p., 1997. Web.
Foote, Robert S. Large scale DNA microsequencing device. United States.
Foote, Robert S. Wed . "Large scale DNA microsequencing device". United States. https://www.osti.gov/servlets/purl/871114.
@article{osti_871114,
title = {Large scale DNA microsequencing device},
author = {Foote, Robert S},
abstractNote = {A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

Patent:

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