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Title: Large scale DNA microsequencing device

A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.
  1. (Oak Ridge, TN)
Issue Date:
OSTI Identifier:
Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN) ORNL
Patent Number(s):
US 5661028
Contract Number:
Research Org:
Country of Publication:
United States
scale; dna; microsequencing; device; microminiature; sequencing; apparatus; method; provide; means; simultaneously; obtaining; sequences; plural; polynucleotide; strands; comprises; microchip; channels; etched; standard; lithographic; procedures; chemical; wet; etching; reaction; separating; section; enclosing; accomplished; bonding; transparent; cover; plate; oligonucleotide; strand; chemically; affixed; alkyl; chain; subsequent; nucleotides; selected; complementary; base; pair; target; nucleotide; produce; family; labelled; channel; separated; following; separation; determined; appropriate; detection; oligonucleotide strand; alkyl chain; chemical wet; transparent cover; detection means; apparatus comprises; cover plate; method provide; wet etching; provide means; target nucleotide; apparatus comprise; complementary base; sequencing apparatus; base pair; scale dna; simultaneously obtaining; nucleotide strand; nucleotide strands; microsequencing device; microminiature sequencing; obtaining sequences; polynucleotide strands; plural polynucleotide; plural channels; dna microsequencing; lithographic procedures; /435/204/216/423/999/