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Title: Microfluidic DNA sample preparation method and device

Abstract

Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.

Inventors:
 [1];  [2];  [3];  [2];  [4];  [5]
  1. Pleasanton, CA
  2. Danville, CA
  3. San Diego, CA
  4. Bellaire, TX
  5. Livermore, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
874265
Patent Number(s):
6352838
Assignee:
The Regents of the Universtiy of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
microfluidic; dna; sample; preparation; method; device; manipulation; molecules; solution; essential; aspect; genetic; analyses; biomedical; assays; identification; hazardous; bacterial; agents; decoding; human; genome; currently; steps; involved; preparing; analysis; performed; manually; time; labor; equipment; intensive; extraction; spores; cells; separation; particles; dust; smoke; cellspore; debris; proteins; strands; specific; lengths; dielectrophoresis; dep; phenomenon; polarizable; move; response; gradient; electric; field; manipulate; separate; automated; fashion; considerably; reducing; expense; allowing; miniaturization; instruments; applications; direct; transport; trapping; allow; varying; electric field; dna molecule; preparation method; /435/204/999/

Citation Formats

Krulevitch, Peter A, Miles, Robin R, Wang, Xiao-Bo, Mariella, Raymond P, Gascoyne, Peter R. C., and Balch, Joseph W. Microfluidic DNA sample preparation method and device. United States: N. p., 2002. Web.
Krulevitch, Peter A, Miles, Robin R, Wang, Xiao-Bo, Mariella, Raymond P, Gascoyne, Peter R. C., & Balch, Joseph W. Microfluidic DNA sample preparation method and device. United States.
Krulevitch, Peter A, Miles, Robin R, Wang, Xiao-Bo, Mariella, Raymond P, Gascoyne, Peter R. C., and Balch, Joseph W. Tue . "Microfluidic DNA sample preparation method and device". United States. https://www.osti.gov/servlets/purl/874265.
@article{osti_874265,
title = {Microfluidic DNA sample preparation method and device},
author = {Krulevitch, Peter A and Miles, Robin R and Wang, Xiao-Bo and Mariella, Raymond P and Gascoyne, Peter R. C. and Balch, Joseph W},
abstractNote = {Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

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