skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Large scale DNA microsequencing device

Abstract

A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means. 11 figs.

Inventors:
Issue Date:
Research Org.:
Lockheed Martin Energy Syst Inc
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
678574
Patent Number(s):
5,944,971
Application Number:
PAN: 8-838,962
Assignee:
Lockheed Martin Energy Research Corp., Oak Ridge, TN (United States) PTO; SCA: 550200; PA: EDB-99:086044; SN: 99002124481
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 31 Aug 1999
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; DNA SEQUENCERS; OLIGONUCLEOTIDES; DESIGN; DNA

Citation Formats

Foote, R.S. Large scale DNA microsequencing device. United States: N. p., 1999. Web.
Foote, R.S. Large scale DNA microsequencing device. United States.
Foote, R.S. Tue . "Large scale DNA microsequencing device". United States.
@article{osti_678574,
title = {Large scale DNA microsequencing device},
author = {Foote, R.S.},
abstractNote = {A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means. 11 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {8}
}