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Title: Large scale DNA microsequencing device

Abstract

A microminiature sequencing apparatus and method provide a means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus cosists of a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means. 17 figs.

Inventors:
Issue Date:
Research Org.:
Lockheed Martin Energy Syst Inc
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
527756
Patent Number(s):
5,661,028
Application Number:
PAN: 8-536,010
Assignee:
Lockheed Martin Energy Systems, Inc., Oak Ridge, TN (United States) PTO; SCA: 550200; PA: EDB-97:127306; SN: 97001843778
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 26 Aug 1997
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCERS; NUCLEOTIDES; DESIGN; DNA SEQUENCING; MOLECULAR BIOLOGY

Citation Formats

Foote, R.S. Large scale DNA microsequencing device. United States: N. p., 1997. Web.
Foote, R.S. Large scale DNA microsequencing device. United States.
Foote, R.S. Tue . "Large scale DNA microsequencing device". United States.
@article{osti_527756,
title = {Large scale DNA microsequencing device},
author = {Foote, R.S.},
abstractNote = {A microminiature sequencing apparatus and method provide a means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus cosists of a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means. 17 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {8}
}