DNA sequencing with pyrophosphatase
Abstract
A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.
- Inventors:
-
- Cambridge, MA
- Chestnut Hill, MA
- Issue Date:
- Research Org.:
- Harvard University
- OSTI Identifier:
- 870336
- Patent Number(s):
- 5498523
- Application Number:
- 08/275,339
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-88ER60688
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- dna; sequencing; pyrophosphatase; kit; solution; extension; oligonucleotide; primer; single-stranded; region; template; molecule; homologous; comprising; agent; reaction; mixture; reduce; amount; pyrophosphate; below; produced; absence; single-stranded region; reaction mixture; dna sequencing; template molecule; oligonucleotide primer; /435/436/
Citation Formats
Tabor, Stanley, and Richardson, Charles C. DNA sequencing with pyrophosphatase. United States: N. p., 1996.
Web.
Tabor, Stanley, & Richardson, Charles C. DNA sequencing with pyrophosphatase. United States.
Tabor, Stanley, and Richardson, Charles C. Mon .
"DNA sequencing with pyrophosphatase". United States. https://www.osti.gov/servlets/purl/870336.
@article{osti_870336,
title = {DNA sequencing with pyrophosphatase},
author = {Tabor, Stanley and Richardson, Charles C},
abstractNote = {A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1996},
month = {Mon Jan 01 00:00:00 EST 1996}
}
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Works referenced in this record:
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
journal, January 1988
- Saiki, R. K.; Gelfand, D. H.; Stoffel, S.
- Science, Vol. 239, Issue 4839, p. 487-491