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Title: Method for performing site-specific affinity fractionation for use in DNA sequencing

Abstract

A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from said array.

Inventors:
 [1];  [1];  [1]
  1. (Moscow, RU)
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL
OSTI Identifier:
872290
Patent Number(s):
5905024
Assignee:
University of Chicago (Chicago, IL) ANL
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; performing; site-specific; affinity; fractionation; dna; sequencing; fractionating; via; interaction; provided; comprising; contacting; cleaved; array; oligonucleotide; molecules; facilitate; hybridization; extracting; hybridized; extracted; specified; base; sequences; complementary; attaching; labeled; re-hybridized; oligomers; provides; multi-step; conversions; chemical; structure; compounds; supplying; polyacrylamide; vessels; separated; hydrophobic; surfaces; immobilizing; plurality; reactants; enzymes; vessel; contains; reactant; predetermined; sequence; sufficient; time; convert; desired; isolating; converted; predetermined sequence; comprising supplying; hybridized dna; cleaved dna; compounds comprising; sequencing dna; base sequences; labeled dna; vessel contains; provided comprising; dna sequencing; sufficient time; oligonucleotide molecules; base sequence; comprising contacting; performing site-specific; via affinity; specific affinity; affinity fractionation; affinity interaction; dna via; contacting cleaved; /435/427/436/999/

Citation Formats

Mirzabekov, Andrei Darievich, Lysov, Yuri Petrovich, and Dubley, Svetlana A. Method for performing site-specific affinity fractionation for use in DNA sequencing. United States: N. p., 1999. Web.
Mirzabekov, Andrei Darievich, Lysov, Yuri Petrovich, & Dubley, Svetlana A. Method for performing site-specific affinity fractionation for use in DNA sequencing. United States.
Mirzabekov, Andrei Darievich, Lysov, Yuri Petrovich, and Dubley, Svetlana A. Fri . "Method for performing site-specific affinity fractionation for use in DNA sequencing". United States. https://www.osti.gov/servlets/purl/872290.
@article{osti_872290,
title = {Method for performing site-specific affinity fractionation for use in DNA sequencing},
author = {Mirzabekov, Andrei Darievich and Lysov, Yuri Petrovich and Dubley, Svetlana A.},
abstractNote = {A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from said array.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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