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Title: Method for performing site-specific affinity fractionation for use in DNA sequencing

Abstract

A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between the cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting the extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to the extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from the array. 14 figs.

Inventors:
; ;
Issue Date:
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6281441
Patent Number(s):
5905024
Application Number:
PPN: US 8-768893
Assignee:
Univ. of Chicago, IL (United States)
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Resource Relation:
Patent File Date: 17 Dec 1996
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AFFINITY; DNA; DNA HYBRIDIZATION; DNA SEQUENCING; MOLECULAR STRUCTURE; OLIGONUCLEOTIDES; BIOTECHNOLOGY; GENETIC ENGINEERING; HYBRIDIZATION; NUCLEIC ACID HYBRIDIZATION; NUCLEIC ACIDS; ORGANIC COMPOUNDS; STRUCTURAL CHEMICAL ANALYSIS; 550200* - Biochemistry

Citation Formats

Mirzabekov, A D, Lysov, Y P, and Dubley, S A. Method for performing site-specific affinity fractionation for use in DNA sequencing. United States: N. p., 1999. Web.
Mirzabekov, A D, Lysov, Y P, & Dubley, S A. Method for performing site-specific affinity fractionation for use in DNA sequencing. United States.
Mirzabekov, A D, Lysov, Y P, and Dubley, S A. Tue . "Method for performing site-specific affinity fractionation for use in DNA sequencing". United States.
@article{osti_6281441,
title = {Method for performing site-specific affinity fractionation for use in DNA sequencing},
author = {Mirzabekov, A D and Lysov, Y P and Dubley, S A},
abstractNote = {A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between the cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting the extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to the extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from the array. 14 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {5}
}