Nanogrid rolling circle DNA sequencing
Patent
·
OSTI ID:1351879
The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.
- Research Organization:
- Harvard Univ., Cambridge, MA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-02ER63445
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Number(s):
- 9,624,538
- Application Number:
- 12/120,541
- OSTI ID:
- 1351879
- Resource Relation:
- Patent File Date: 2008 May 14
- Country of Publication:
- United States
- Language:
- English
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