Nanogrid rolling circle DNA sequencing

Church, George M.; Porreca, Gregory J.; Shendure, Jay; Rosenbaum, Abraham Meir

Title: Nanogrid rolling circle DNA sequencing

Patent · Tue Apr 18 00:00:00 EDT 2017

OSTI ID:1351879

Church, George M.; Porreca, Gregory J.; Shendure, Jay; Rosenbaum, Abraham Meir

The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.

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Research Organization:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-02ER63445

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Number(s):: 9,624,538

Application Number:: 12/120,541

OSTI ID:: 1351879

Resource Relation:: Patent File Date: 2008 May 14

Country of Publication:: United States

Language:: English

References (24)

Circle-to-circle amplification for precise and sensitive DNA analysis Dahl, F.; Baner, J.; Gullberg, M. Proceedings of the National Academy of Sciences, Vol. 101, Issue 13, p. 4548-4553 https://doi.org/10.1073/pnas.0400834101	journal	March 2004
Rolling-circle amplification in DNA diagnostics the power of simplicity Demidov, Vadim V. Expert Review of Molecular Diagnostics, Vol. 2, Issue 6, p. 542-548 https://doi.org/10.1586/14737159.2.6.542	journal	November 2002
Exploring the DNA-binding specificities of zinc fingers with DNA microarrays Bulyk, M. L.; Huang, X.; Choo, Y. Proceedings of the National Academy of Sciences, Vol. 98, Issue 13, p. 7158-7163 https://doi.org/10.1073/pnas.111163698	journal	June 2001
Vapor-Phase Self-Assembled Monolayer for Improved Mold Release in Nanoimprint Lithography Jung, Gun-Young; Li, Zhiyong; Wu, Wei Langmuir, Vol. 21, Issue 4, p. 1158-1161 https://doi.org/10.1021/la0476938	journal	February 2005
Multiplexed protein profiling on microarrays by rolling-circle amplification Schweitzer, Barry; Roberts, Scott; Grimwade, Brian Nature Biotechnology, Vol. 20, Issue 4, p. 359-365 https://doi.org/10.1038/nbt0402-359	journal	April 2002
Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification An, Lixin; Tang, Wen; Ranalli, Tamara A. Journal of Biological Chemistry, Vol. 280, Issue 32, p. 28952-28958 https://doi.org/10.1074/jbc.M503096200	journal	June 2005
Homogeneous assay system using the nuclease activity of a nucleic acid polymerase Gelfand, David H.; Holland, Pamela M.; Saiki, Randall K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5210015 US Patent Document 5,210,015	patent	May 1993
High-throughput genotyping assay approaches Kwok, Pui-Yan Pharmacogenomics, Vol. 1, Issue 1, p. 95-100 https://doi.org/10.1517/14622416.1.1.95	journal	February 2000
Improved Pattern Transfer in Nanoimprint Lithography at 30 nm Half-Pitch by Substrate−Surface Functionalization Jung, Gun-Young; Li, Zhiyong; Wu, Wei Langmuir, Vol. 21, Issue 14, p. 6127-6130 https://doi.org/10.1021/la050021c	journal	July 2005
Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir–Blodgett lithography Lenhert, Steven; Meier, Marie-Beatrice; Meyer, Ulrich Biomaterials, Vol. 26, Issue 5, p. 563-570 https://doi.org/10.1016/j.biomaterials.2004.02.068	journal	February 2005
Transition of MEMS Technology to Nanofabrication Luesebrink, Helge; Glinsner, Thomas; Jakeway, Stephen C. Journal of Nanoscience and Nanotechnology, Vol. 5, Issue 6, p. 864-868 https://doi.org/10.1166/jnn.2005.129	journal	June 2005
Fabrication of Large Number Density Platinum Nanowire Arrays by Size Reduction Lithography and Nanoimprint Lithography Yan, X.-M.; Kwon, S.; Contreras, A. M. Nano Letters, Vol. 5, Issue 4, p. 745-748 https://doi.org/10.1021/nl050228q	journal	April 2005
Replica amplification of nucleic acid arrays Church, George M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6432360 US Patent Document 6,432,360	patent	August 2002
Process for amplifying, detecting, and/or cloning nucleic acid sequences Mullis, Kary B.; Erlich, Henry A.; Arnheim, Norman https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4800159 US Patent Document 4,800,159	patent	January 1989
Mutation detection and single-molecule counting using isothermal rolling-circleamplification Lizardi, Paul M.; Huang, Xiaohua; Zhu, Zhengrong Nature Genetics, Vol. 19, Issue 3, p. 225-232 https://doi.org/10.1038/898	journal	July 1998
Process for amplifying nucleic acid sequences Mullis, Kary B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683202 US Patent Document 4,683,202	patent	July 1987
Nanopattern-induced changes in morphology and motility of smooth muscle cells Yim, E.; Reano, R.; Pang, S. Biomaterials, Vol. 26, Issue 26, p. 5405-5413 https://doi.org/10.1016/j.biomaterials.2005.01.058	journal	September 2005
Methods for making single molecule arrays Drmanac, Radoje; Callow, Matthew J.; Drmanac, Snezana https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8133719 US Patent Document 8,133,719	patent	March 2012
Replica amplification of nucleic acid arrays Church, George M.; Mitra, Robi D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7785790 US Patent Document 7,785,790	patent	August 2010
Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme Mullis, Kary B.; Erlich, Henry A.; Gelfand, David H. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4965188 US Patent Document 4,965,188	patent	October 1990
Rolling circle replication reporter systems Lizardi, Paul M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5854033 US Patent Document 5,854,033	patent	December 1998
Process for amplifying, detecting, and/or-cloning nucleic acid sequences Mullis, Kary B.; Erlich, Henry A.; Arnheim, Norman https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683195 US Patent Document 4,683,195	patent	July 1987
Monitoring amplification of DNA during PCR Wittwer, Carl T.; Ririe, Kirk M.; Rasmussen, Randy P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6174670 US Patent Document 6,174,670	patent	January 2001
Nucleic acid sequence amplification methods Kacian, Daniel L.; Fultz, Timothy J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5399491 US Patent Document 5,399,491	patent	March 1995

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