Nanogrid rolling circle DNA sequencing

Church, George M.; Porreca, Gregory J.; Shendure, Jay; Rosenbaum, Abraham Meir

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Title: Nanogrid rolling circle DNA sequencing

Abstract

The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.

Inventors:: Church, George M.; Porreca, Gregory J.; Shendure, Jay; Rosenbaum, Abraham Meir

Issue Date:: Tue Apr 18 00:00:00 EDT 2017

Research Org.:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1351879

Patent Number(s):: 9624538

Application Number:: 12/120,541

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6874 - involving nucleic acid arrays, e.g. sequencing by hybridisation
C12Q2531/125 - Rolling circle
C12Q2563/155 - Particles of a defined size, e.g. nanoparticles
C12Q2565/513 - characterised by the pattern of the arrayed oligonucleotides

Show less

DOE Contract Number:: FG02-02ER63445

Resource Type:: Patent

Resource Relation:: Patent File Date: 2008 May 14

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Church, George M., Porreca, Gregory J., Shendure, Jay, and Rosenbaum, Abraham Meir. Nanogrid rolling circle DNA sequencing.  United States: N. p., 2017. 
        Web.

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                    Church, George M., Porreca, Gregory J., Shendure, Jay, & Rosenbaum, Abraham Meir. Nanogrid rolling circle DNA sequencing.  United States.

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                    Church, George M., Porreca, Gregory J., Shendure, Jay, and Rosenbaum, Abraham Meir. Tue .  
        "Nanogrid rolling circle DNA sequencing".  United States.  https://www.osti.gov/servlets/purl/1351879.

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@article{osti_1351879,

  title        = {Nanogrid rolling circle DNA sequencing},

  author       = {Church, George M. and Porreca, Gregory J. and Shendure, Jay and Rosenbaum, Abraham Meir},

  abstractNote = {The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Apr 18 00:00:00 EDT 2017},

  month        = {Tue Apr 18 00:00:00 EDT 2017}

}

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Similar Records

Title: Nanogrid rolling circle DNA sequencing

Abstract

Citation Formats

Circle-to-circle amplification for precise and sensitive DNA analysis journal, March 2004

Rolling-circle amplification in DNA diagnostics the power of simplicity journal, November 2002

Exploring the DNA-binding specificities of zinc fingers with DNA microarrays journal, June 2001

Vapor-Phase Self-Assembled Monolayer for Improved Mold Release in Nanoimprint Lithography journal, February 2005

Multiplexed protein profiling on microarrays by rolling-circle amplification journal, April 2002

Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification journal, June 2005

Homogeneous assay system using the nuclease activity of a nucleic acid polymerase patent, May 1993

High-throughput genotyping assay approaches journal, February 2000

Improved Pattern Transfer in Nanoimprint Lithography at 30 nm Half-Pitch by Substrate−Surface Functionalization journal, July 2005

Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir–Blodgett lithography journal, February 2005

Transition of MEMS Technology to Nanofabrication journal, June 2005

Fabrication of Large Number Density Platinum Nanowire Arrays by Size Reduction Lithography and Nanoimprint Lithography journal, April 2005

Replica amplification of nucleic acid arrays patent, August 2002

Process for amplifying, detecting, and/or cloning nucleic acid sequences patent, January 1989

Mutation detection and single-molecule counting using isothermal rolling-circleamplification journal, July 1998

Process for amplifying nucleic acid sequences patent, July 1987

Nanopattern-induced changes in morphology and motility of smooth muscle cells journal, September 2005

Methods for making single molecule arrays patent, March 2012

Replica amplification of nucleic acid arrays patent, August 2010

Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme patent, October 1990

Rolling circle replication reporter systems patent, December 1998

Process for amplifying, detecting, and/or-cloning nucleic acid sequences patent, July 1987

Monitoring amplification of DNA during PCR patent, January 2001

Nucleic acid sequence amplification methods patent, March 1995

Circle-to-circle amplification for precise and sensitive DNA analysis
journal, March 2004

Rolling-circle amplification in DNA diagnostics the power of simplicity
journal, November 2002

Exploring the DNA-binding specificities of zinc fingers with DNA microarrays
journal, June 2001

Vapor-Phase Self-Assembled Monolayer for Improved Mold Release in Nanoimprint Lithography
journal, February 2005

Multiplexed protein profiling on microarrays by rolling-circle amplification
journal, April 2002

Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification
journal, June 2005

Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
patent, May 1993

High-throughput genotyping assay approaches
journal, February 2000

Improved Pattern Transfer in Nanoimprint Lithography at 30 nm Half-Pitch by Substrate−Surface Functionalization
journal, July 2005

Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir–Blodgett lithography
journal, February 2005

Transition of MEMS Technology to Nanofabrication
journal, June 2005

Fabrication of Large Number Density Platinum Nanowire Arrays by Size Reduction Lithography and Nanoimprint Lithography
journal, April 2005

Replica amplification of nucleic acid arrays
patent, August 2002

Process for amplifying, detecting, and/or cloning nucleic acid sequences
patent, January 1989

Mutation detection and single-molecule counting using isothermal rolling-circleamplification
journal, July 1998

Process for amplifying nucleic acid sequences
patent, July 1987

Nanopattern-induced changes in morphology and motility of smooth muscle cells
journal, September 2005

Methods for making single molecule arrays
patent, March 2012

Replica amplification of nucleic acid arrays
patent, August 2010

Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
patent, October 1990

Rolling circle replication reporter systems
patent, December 1998

Process for amplifying, detecting, and/or-cloning nucleic acid sequences
patent, July 1987

Monitoring amplification of DNA during PCR
patent, January 2001

Nucleic acid sequence amplification methods
patent, March 1995