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Title: Nanogrid rolling circle DNA sequencing

The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.
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Issue Date:
OSTI Identifier:
President and Fellows of Harvard College CHO
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2008 May 14
Research Org:
President and Fellows of Harvard College, Cambridge, MA (United States)
Sponsoring Org:
Country of Publication:
United States

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