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Title: Nanogrid rolling circle DNA sequencing

The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1351879
Assignee:
President and Fellows of Harvard College CHO
Patent Number(s):
9,624,538
Application Number:
12/120,541
Contract Number:
FG02-02ER63445
Resource Relation:
Patent File Date: 2008 May 14
Research Org:
President and Fellows of Harvard College, Cambridge, MA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

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