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Title: Nanogrid rolling circle DNA sequencing

Abstract

The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.

Inventors:
; ; ;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1351879
Patent Number(s):
9624538
Application Number:
12/120,541
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 May 14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Church, George M., Porreca, Gregory J., Shendure, Jay, and Rosenbaum, Abraham Meir. Nanogrid rolling circle DNA sequencing. United States: N. p., 2017. Web.
Church, George M., Porreca, Gregory J., Shendure, Jay, & Rosenbaum, Abraham Meir. Nanogrid rolling circle DNA sequencing. United States.
Church, George M., Porreca, Gregory J., Shendure, Jay, and Rosenbaum, Abraham Meir. Tue . "Nanogrid rolling circle DNA sequencing". United States. https://www.osti.gov/servlets/purl/1351879.
@article{osti_1351879,
title = {Nanogrid rolling circle DNA sequencing},
author = {Church, George M. and Porreca, Gregory J. and Shendure, Jay and Rosenbaum, Abraham Meir},
abstractNote = {The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {4}
}

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Works referenced in this record:

Circle-to-circle amplification for precise and sensitive DNA analysis
journal, March 2004


Rolling-circle amplification in DNA diagnostics the power of simplicity
journal, November 2002


Exploring the DNA-binding specificities of zinc fingers with DNA microarrays
journal, June 2001


Vapor-Phase Self-Assembled Monolayer for Improved Mold Release in Nanoimprint Lithography
journal, February 2005


Multiplexed protein profiling on microarrays by rolling-circle amplification
journal, April 2002


Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification
journal, June 2005


High-throughput genotyping assay approaches
journal, February 2000


Improved Pattern Transfer in Nanoimprint Lithography at 30 nm Half-Pitch by Substrate−Surface Functionalization
journal, July 2005


Osteoblast alignment, elongation and migration on grooved polystyrene surfaces patterned by Langmuir–Blodgett lithography
journal, February 2005


Transition of MEMS Technology to Nanofabrication
journal, June 2005


Fabrication of Large Number Density Platinum Nanowire Arrays by Size Reduction Lithography and Nanoimprint Lithography
journal, April 2005


Mutation detection and single-molecule counting using isothermal rolling-circleamplification
journal, July 1998


Nanopattern-induced changes in morphology and motility of smooth muscle cells
journal, September 2005