Cloning vector
Abstract
A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site. 2 figures.
- Inventors:
- Issue Date:
- OSTI Identifier:
- 6649482
- Patent Number(s):
- 5376549
- Application Number:
- PPN: US 8-014944
- Assignee:
- Wisconsin Alumni Research Foundation, Madison, WI (United States)
- DOE Contract Number:
- FG02-90ER61026
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 5 Feb 1993
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; BACTERIOPHAGES; DNA SEQUENCING; DNA-CLONING; DNA REPAIR; ENDONUCLEASES; TRANSCRIPTION FACTORS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BIOTECHNOLOGY; CLONING; DNA HYBRIDIZATION; DNA-ASE; ENZYMES; ESTERASES; GENETIC ENGINEERING; HYBRIDIZATION; HYDROLASES; MICROORGANISMS; NUCLEIC ACID HYBRIDIZATION; ORGANIC COMPOUNDS; PARASITES; PHOSPHODIESTERASES; PROTEINS; REPAIR; STRUCTURAL CHEMICAL ANALYSIS; VIRUSES; 550200* - Biochemistry
Citation Formats
Guilfoyle, R A, and Smith, L M. Cloning vector. United States: N. p., 1994.
Web.
Guilfoyle, R A, & Smith, L M. Cloning vector. United States.
Guilfoyle, R A, and Smith, L M. Tue .
"Cloning vector". United States.
@article{osti_6649482,
title = {Cloning vector},
author = {Guilfoyle, R A and Smith, L M},
abstractNote = {A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site. 2 figures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 27 00:00:00 EST 1994},
month = {Tue Dec 27 00:00:00 EST 1994}
}
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