Cloning vector
Abstract
A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site.
- Inventors:
-
- Madison, WI
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- OSTI Identifier:
- 869677
- Patent Number(s):
- 5376549
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-90ER61026
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- cloning; vector; comprising; filamentous; phage; sequence; containing; copy; sequences; propagate; disclosed; contains; downstream; promoter; capable; promoting; transcription; bacterial; host; preferred; form; m13; additionally; restriction; endonuclease; site; located; manner; substantially; inactivate; dna; inserted; bacterial host; dna sequence; preferred form; cloning vector; restriction site; filamentous phage; restriction endonuclease; vector comprising; /435/
Citation Formats
Guilfoyle, Richard A, and Smith, Lloyd M. Cloning vector. United States: N. p., 1994.
Web.
Guilfoyle, Richard A, & Smith, Lloyd M. Cloning vector. United States.
Guilfoyle, Richard A, and Smith, Lloyd M. Sat .
"Cloning vector". United States. https://www.osti.gov/servlets/purl/869677.
@article{osti_869677,
title = {Cloning vector},
author = {Guilfoyle, Richard A and Smith, Lloyd M},
abstractNote = {A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {1}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Regulation of bacteriophage f1 DNA replication
journal, September 1988
- Fulford, Wilder; Model, Peter
- Journal of Molecular Biology, Vol. 203, Issue 1
Rapid shotgeun cloning utillizing the two base recongition endonuclease Cvi JI
journal, January 1992
- Fitzgerald, Michael C.; Skowron, Peter; Van Etten, James L.
- Nucleic Acids Research, Vol. 20, Issue 14
EcoK selection vectors for shotgun cloning into M13 and deletion mutagenesls
journal, January 1985
- Waye, Mary M. Y.; Verhoeyen, Martine E.; Jones, Peter T.
- Nucleic Acids Research, Vol. 13, Issue 23
Nucleotide sequence and genome organisation of filamentous bacteriophages f1 and fd
journal, December 1981
- Beck, Ewald; Zink, Barbara
- Gene, Vol. 16, Issue 1-3
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
journal, May 1986
- Studier, F. William; Moffatt, Barbara A.
- Journal of Molecular Biology, Vol. 189, Issue 1, p. 113-130
A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy.
journal, March 1992
- Pierce, J. C.; Sauer, B.; Sternberg, N.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 6