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Title: Cloning vector

Abstract

A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site.

Inventors:
 [1];  [1]
  1. Madison, WI
Issue Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
OSTI Identifier:
869677
Patent Number(s):
5376549
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-90ER61026
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
cloning; vector; comprising; filamentous; phage; sequence; containing; copy; sequences; propagate; disclosed; contains; downstream; promoter; capable; promoting; transcription; bacterial; host; preferred; form; m13; additionally; restriction; endonuclease; site; located; manner; substantially; inactivate; dna; inserted; bacterial host; dna sequence; preferred form; cloning vector; restriction site; filamentous phage; restriction endonuclease; vector comprising; /435/

Citation Formats

Guilfoyle, Richard A, and Smith, Lloyd M. Cloning vector. United States: N. p., 1994. Web.
Guilfoyle, Richard A, & Smith, Lloyd M. Cloning vector. United States.
Guilfoyle, Richard A, and Smith, Lloyd M. Sat . "Cloning vector". United States. https://www.osti.gov/servlets/purl/869677.
@article{osti_869677,
title = {Cloning vector},
author = {Guilfoyle, Richard A and Smith, Lloyd M},
abstractNote = {A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {1}
}

Works referenced in this record:

Regulation of bacteriophage f1 DNA replication
journal, September 1988


Rapid shotgeun cloning utillizing the two base recongition endonuclease Cvi JI
journal, January 1992


EcoK selection vectors for shotgun cloning into M13 and deletion mutagenesls
journal, January 1985


Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
journal, May 1986


A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy.
journal, March 1992