Cloning vector
A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site.
- Issue Date:
- OSTI Identifier:
- 869677
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI) CHO
- Patent Number(s):
- US 5376549
- Contract Number:
- FG02-90ER61026
- Research Org:
- University of Wisconsin
- Country of Publication:
- United States
- Language:
- English
- Subject:
- cloning; vector; comprising; filamentous; phage; sequence; containing; copy; sequences; propagate; disclosed; contains; downstream; promoter; capable; promoting; transcription; bacterial; host; preferred; form; m13; additionally; restriction; endonuclease; site; located; manner; substantially; inactivate; dna; inserted; bacterial host; dna sequence; preferred form; cloning vector; restriction site; filamentous phage; restriction endonuclease; vector comprising; /435/
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