Transposon-containing DNA cloning vector and uses thereof

Berg, C M; Berg, D E; Wang, G

Title: Transposon-containing DNA cloning vector and uses thereof

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Abstract

The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed. 4 figs.

Inventors:: Berg, C M; Berg, D E; Wang, G

Issue Date:: 1997-07-08

Research Org.:: Univ. of Washington, Seattle, WA (United States); Univ. of Connecticut, Storrs, CT (United States)

Sponsoring Org.:: USDOE, Washington, DC (United States)

OSTI Identifier:: 512421

Patent Number(s):: 5645991

Application Number:: PAN: 8-403,582

Assignee:: Univ. of Connecticut, Storrs, CT (United States); Washington Univ., St. Louis, MO (United States)

DOE Contract Number:: FG02-89ER60862; FG02-90ER61000

Resource Type:: Patent

Resource Relation:: Other Information: PBD: 8 Jul 1997

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; PLASMIDS; GENETIC ENGINEERING; DNA SEQUENCING; TRANSPOSONS

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                    Berg, C M, Berg, D E, and Wang, G. Transposon-containing DNA cloning vector and uses thereof.  United States: N. p., 1997. 
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                    Berg, C M, Berg, D E, & Wang, G. Transposon-containing DNA cloning vector and uses thereof.  United States.

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                    Berg, C M, Berg, D E, and Wang, G. Tue .  
        "Transposon-containing DNA cloning vector and uses thereof".  United States.

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@article{osti_512421,

  title        = {Transposon-containing DNA cloning vector and uses thereof},

  author       = {Berg, C M and Berg, D E and Wang, G},

  abstractNote = {The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed. 4 figs.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1997},

  month        = {7}

}

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Transposon-containing DNA cloning vector and uses thereof

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