skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Transposon-containing DNA cloning vector and uses thereof

Abstract

The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed. 4 figs.

Inventors:
; ;
Issue Date:
Research Org.:
Washington University; University of Connecticut
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
512421
Patent Number(s):
5,645,991
Application Number:
PAN: 8-403,582
Assignee:
Univ. of Connecticut, Storrs, CT (United States); Washington Univ., St. Louis, MO (United States) PTO; SCA: 550200; PA: EDB-97:113584; SN: 97001827906
DOE Contract Number:  
FG02-89ER60862; FG02-90ER61000
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 8 Jul 1997
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA-CLONING; PLASMIDS; GENETIC ENGINEERING; DNA SEQUENCING; TRANSPOSONS

Citation Formats

Berg, C.M., Berg, D.E., and Wang, G. Transposon-containing DNA cloning vector and uses thereof. United States: N. p., 1997. Web.
Berg, C.M., Berg, D.E., & Wang, G. Transposon-containing DNA cloning vector and uses thereof. United States.
Berg, C.M., Berg, D.E., and Wang, G. Tue . "Transposon-containing DNA cloning vector and uses thereof". United States.
@article{osti_512421,
title = {Transposon-containing DNA cloning vector and uses thereof},
author = {Berg, C.M. and Berg, D.E. and Wang, G.},
abstractNote = {The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {7}
}