Transposon-containing DNA cloning vector and uses thereof
Abstract
The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.
- Inventors:
-
- W. Willington, CT
- St. Louis, MO
- Storrs, CT
- Issue Date:
- Research Org.:
- Univ. of Washington, Seattle, WA (United States); Univ. of Connecticut, Storrs, CT (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 871041
- Patent Number(s):
- 5645991
- Assignee:
- Univ. of Connecticut (Storrs, CT); Washington University (St. Louis, MO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-89ER60862; FG02-90ER61000
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- transposon-containing; dna; cloning; vector; discloses; rapid; method; restriction; mapping; sequencing; localizing; genetic; features; segment; deoxyribonucleic; acid; 42; kb; size; comprises; specialized; isolating; nested; deletions; direction; vivo; intramolecular; transposition; cloned; plasmid; prepared; disclosed; nucleic acid; dna segment; cloning vector; cloned dna; rapid method; nested deletions; /435/
Citation Formats
Berg, Claire M, Berg, Douglas E, and Wang, Gan. Transposon-containing DNA cloning vector and uses thereof. United States: N. p., 1997.
Web.
Berg, Claire M, Berg, Douglas E, & Wang, Gan. Transposon-containing DNA cloning vector and uses thereof. United States.
Berg, Claire M, Berg, Douglas E, and Wang, Gan. Wed .
"Transposon-containing DNA cloning vector and uses thereof". United States. https://www.osti.gov/servlets/purl/871041.
@article{osti_871041,
title = {Transposon-containing DNA cloning vector and uses thereof},
author = {Berg, Claire M and Berg, Douglas E and Wang, Gan},
abstractNote = {The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}
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