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Title: Transposon-containing DNA cloning vector and uses thereof

The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.
 [1];  [2];  [3]
  1. (W. Willington, CT)
  2. (St. Louis, MO)
  3. (Storrs, CT)
Issue Date:
OSTI Identifier:
Univ. of Connecticut (Storrs, CT); Washington University (St. Louis, MO) CHO
Patent Number(s):
US 5645991
Contract Number:
FG02-89ER60862; FG02-90ER61000
Research Org:
Washington University; University of Connecticut
Sponsoring Org:
Country of Publication:
United States
transposon-containing; dna; cloning; vector; discloses; rapid; method; restriction; mapping; sequencing; localizing; genetic; features; segment; deoxyribonucleic; acid; 42; kb; size; comprises; specialized; isolating; nested; deletions; direction; vivo; intramolecular; transposition; cloned; plasmid; prepared; disclosed; nucleic acid; dna segment; cloning vector; cloned dna; rapid method; nested deletions; /435/