Cloning and expression vectors and systems

Studier, F. William

Title: Cloning and expression vectors and systems

Patent · Tue Apr 04 00:00:00 EDT 2023

OSTI ID:1998257

Studier, F. William

Vectors for cloning, maintaining and expressing a wide range of coding sequences in inducible T7 expression systems in Escherichia coli expression hosts are disclosed herein. Target genes that can be stably maintained and expressed include those that specify proteins that are highly toxic to the host cell. Different configurations of vectors and expression hosts provide different rates of transcription and translation of target genes and therefore different rates of accumulation of target proteins. Methods for cloning by asymmetric ligation and co-expression of more than one target protein in a single vector are also disclosed, as are variants of BL21(DE3) having lower basal transcription by T7 RNA polymerase.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: SC0012704

Assignee:: Brookhaven Science Associates, LLC (Upton, NY)

Patent Number(s):: 11,618,899

Application Number:: 16/077,014

OSTI ID:: 1998257

Resource Relation:: Patent File Date: 02/08/2017

Country of Publication:: United States

Language:: English

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