Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth

Myers, Kevin S.; Gasch, Audrey P.

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Title: Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth

Abstract

The present invention relates to materials and methods for the production of ethanol. More particularly, the present invention provides genetically modified strains of Saccharomyces cerevisiae exhibiting decreased level of BCY1 protein activity and capable of anaerobic fermentation of xylose into ethanol without the need for cell growth. Also provided are methods of using such genetically engineered yeast strains for improved anaerobic xylose fermentation in the yeast for industrial-scale production of various fuels, chemical feedstocks, and synthetic polymers.

Inventors:: Myers, Kevin S.; Gasch, Audrey P.

Issue Date:: 2022-05-10

Research Org.:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1892912

Patent Number(s):: 11326174

Application Number:: 16/542,758

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/395 - from Saccharomyces

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/81 - for yeasts
C12N15/905 - {in yeast}

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/10 - substrate containing cellulosic material

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
Y02E50/10 - Biofuels

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DOE Contract Number:: FC02-07ER64494

Resource Type:: Patent

Resource Relation:: Patent File Date: 08/16/2019

Country of Publication:: United States

Language:: English

Citation Formats


                    Myers, Kevin S., and Gasch, Audrey P. Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth.  United States: N. p., 2022. 
        Web.

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                    Myers, Kevin S., & Gasch, Audrey P. Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth.  United States.

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                    Myers, Kevin S., and Gasch, Audrey P. Tue .  
        "Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth".  United States.  https://www.osti.gov/servlets/purl/1892912.

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@article{osti_1892912,

  title        = {Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth},

  author       = {Myers, Kevin S. and Gasch, Audrey P.},

  abstractNote = {The present invention relates to materials and methods for the production of ethanol. More particularly, the present invention provides genetically modified strains of Saccharomyces cerevisiae exhibiting decreased level of BCY1 protein activity and capable of anaerobic fermentation of xylose into ethanol without the need for cell growth. Also provided are methods of using such genetically engineered yeast strains for improved anaerobic xylose fermentation in the yeast for industrial-scale production of various fuels, chemical feedstocks, and synthetic polymers.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2022},

  month        = {5}

}

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Works referenced in this record:

How nutritional status signalling coordinates metabolism and lignocellulolytic enzyme secretion
journal, November 2014

Brown, Neil Andrew; Ries, Laure Nicolas Annick; Goldman, Gustavo Henrique
Fungal Genetics and Biology, Vol. 72
https://doi.org/10.1016/j.fgb.2014.06.012

Modifying Yeast Tolerance to Inhibitory Conditions of Ethanol Production Processes
journal, November 2015

Caspeta, Luis; Castillo, Tania; Nielsen, Jens
Frontiers in Bioengineering and Biotechnology, Vol. 3
https://doi.org/10.3389/fbioe.2015.00184

An expanded tool kit for the auxin-inducible degron system in budding yeast: A tool kit for the AID system
journal, July 2013

Morawska, Magdalena; Ulrich, Helle D.
Yeast, Vol. 30, Issue 9
https://doi.org/10.1002/yea.2967

The localization and concentration of thePDE2-encoded high-affinity cAMP phosphodiesterase is regulated by cAMP-dependent protein kinase A in the yeastSaccharomyces cerevisiae
journal, March 2010

Hu, Yun; Liu, Enkai; Bai, Xiaojia
FEMS Yeast Research, Vol. 10, Issue 2
https://doi.org/10.1111/j.1567-1364.2009.00598.x

Deletion of BCY1 from the Saccharomyces cerevisiae Genome Is Semidominant and Induces Autolytic Phenotypes Suitable for Improvement of Sparkling Wines
journal, April 2006

Tabera, Laura; Muñoz, Rosario; Gonzalez, Ramon
Applied and Environmental Microbiology, Vol. 72, Issue 4
https://doi.org/10.1128/AEM.72.4.2351-2358.2006

Yeast bcy1 mutants with stationary phase-specific defects
journal, September 1997

Peck, Vickie M.; Fuge, Edwina K.; Padilla, Pamela A.
Current Genetics, Vol. 32, Issue 2
https://doi.org/10.1007/s002940050251

Over-expression of AZF1 improves the rate of anaerobic xylose fermentation in engineered yeast strains
patent-application, April 2019

Gasch, Audrey P.; Myers, Kevin
US Patent Application 16/156463; 20190106469
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20190106469

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Similar Records

Title: Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth

Abstract

Citation Formats

How nutritional status signalling coordinates metabolism and lignocellulolytic enzyme secretion journal, November 2014

Modifying Yeast Tolerance to Inhibitory Conditions of Ethanol Production Processes journal, November 2015

An expanded tool kit for the auxin-inducible degron system in budding yeast: A tool kit for the AID system journal, July 2013

The localization and concentration of thePDE2-encoded high-affinity cAMP phosphodiesterase is regulated by cAMP-dependent protein kinase A in the yeastSaccharomyces cerevisiae journal, March 2010

Deletion of BCY1 from the Saccharomyces cerevisiae Genome Is Semidominant and Induces Autolytic Phenotypes Suitable for Improvement of Sparkling Wines journal, April 2006

Yeast bcy1 mutants with stationary phase-specific defects journal, September 1997

Over-expression of AZF1 improves the rate of anaerobic xylose fermentation in engineered yeast strains patent-application, April 2019

How nutritional status signalling coordinates metabolism and lignocellulolytic enzyme secretion
journal, November 2014

Modifying Yeast Tolerance to Inhibitory Conditions of Ethanol Production Processes
journal, November 2015

An expanded tool kit for the auxin-inducible degron system in budding yeast: A tool kit for the AID system
journal, July 2013

The localization and concentration of thePDE2-encoded high-affinity cAMP phosphodiesterase is regulated by cAMP-dependent protein kinase A in the yeastSaccharomyces cerevisiae
journal, March 2010

Deletion of BCY1 from the Saccharomyces cerevisiae Genome Is Semidominant and Induces Autolytic Phenotypes Suitable for Improvement of Sparkling Wines
journal, April 2006

Yeast bcy1 mutants with stationary phase-specific defects
journal, September 1997

Over-expression of AZF1 improves the rate of anaerobic xylose fermentation in engineered yeast strains
patent-application, April 2019