Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth
Abstract
The present invention relates to materials and methods for the production of ethanol. More particularly, the present invention provides genetically modified strains of Saccharomyces cerevisiae exhibiting decreased level of BCY1 protein activity and capable of anaerobic fermentation of xylose into ethanol without the need for cell growth. Also provided are methods of using such genetically engineered yeast strains for improved anaerobic xylose fermentation in the yeast for industrial-scale production of various fuels, chemical feedstocks, and synthetic polymers.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1892912
- Patent Number(s):
- 11326174
- Application Number:
- 16/542,758
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FC02-07ER64494
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 08/16/2019
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Myers, Kevin S., and Gasch, Audrey P. Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth. United States: N. p., 2022.
Web.
Myers, Kevin S., & Gasch, Audrey P. Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth. United States.
Myers, Kevin S., and Gasch, Audrey P. Tue .
"Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth". United States. https://www.osti.gov/servlets/purl/1892912.
@article{osti_1892912,
title = {Engineered yeast strains enabling anaerobic xylose fermentation decoupled from microbial growth},
author = {Myers, Kevin S. and Gasch, Audrey P.},
abstractNote = {The present invention relates to materials and methods for the production of ethanol. More particularly, the present invention provides genetically modified strains of Saccharomyces cerevisiae exhibiting decreased level of BCY1 protein activity and capable of anaerobic fermentation of xylose into ethanol without the need for cell growth. Also provided are methods of using such genetically engineered yeast strains for improved anaerobic xylose fermentation in the yeast for industrial-scale production of various fuels, chemical feedstocks, and synthetic polymers.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 10 00:00:00 EDT 2022},
month = {Tue May 10 00:00:00 EDT 2022}
}
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Works referenced in this record:
How nutritional status signalling coordinates metabolism and lignocellulolytic enzyme secretion
journal, November 2014
- Brown, Neil Andrew; Ries, Laure Nicolas Annick; Goldman, Gustavo Henrique
- Fungal Genetics and Biology, Vol. 72
Modifying Yeast Tolerance to Inhibitory Conditions of Ethanol Production Processes
journal, November 2015
- Caspeta, Luis; Castillo, Tania; Nielsen, Jens
- Frontiers in Bioengineering and Biotechnology, Vol. 3
An expanded tool kit for the auxin-inducible degron system in budding yeast: A tool kit for the AID system
journal, July 2013
- Morawska, Magdalena; Ulrich, Helle D.
- Yeast, Vol. 30, Issue 9
The localization and concentration of thePDE2-encoded high-affinity cAMP phosphodiesterase is regulated by cAMP-dependent protein kinase A in the yeastSaccharomyces cerevisiae
journal, March 2010
- Hu, Yun; Liu, Enkai; Bai, Xiaojia
- FEMS Yeast Research, Vol. 10, Issue 2
Deletion of
BCY1
from the
Saccharomyces cerevisiae
Genome Is Semidominant and Induces Autolytic Phenotypes Suitable for Improvement of Sparkling Wines
journal, April 2006
- Tabera, Laura; Muñoz, Rosario; Gonzalez, Ramon
- Applied and Environmental Microbiology, Vol. 72, Issue 4
Yeast bcy1 mutants with stationary phase-specific defects
journal, September 1997
- Peck, Vickie M.; Fuge, Edwina K.; Padilla, Pamela A.
- Current Genetics, Vol. 32, Issue 2
Over-expression of AZF1 improves the rate of anaerobic xylose fermentation in engineered yeast strains
patent-application, April 2019
- Gasch, Audrey P.; Myers, Kevin
- US Patent Application 16/156463; 20190106469