skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Over-expression of AZF1 improves the rate of anaerobic xylose fermentation in engineered yeast strains

Patent ·
OSTI ID:1987170

The present invention provides an isolated AZF1 gene sequence, recombinant vectors, and recombinant yeast which are useful in methods of enhanced biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast with isolated AZF1 gene sequence useful for biofuel production are also provided.

Research Organization:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC02-07ER64494
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Number(s):
11,591,376
Application Number:
16/156,463
OSTI ID:
1987170
Resource Relation:
Patent File Date: 10/10/2018
Country of Publication:
United States
Language:
English

References (22)

Xylose Assimilation for the Efficient Production of Biofuels and Chemicals by Engineered Saccharomyces cerevisiae journal November 2020
Rewired cellular signaling coordinates sugar and hypoxic responses for anaerobic xylose fermentation in yeast journal March 2019
Methods for Improving Secondary Metabolite Production of Fungi patent-application November 2006
AZF1 Is a Glucose-Dependent Positive Regulator of CLN3 Transcription in Saccharomyces cerevisiae journal March 2002
Galactosyltransferase patent-application February 2010
Codon Optimization of a Synthetic Gene(s) for Protein Expression patent-application August 2014
A new nuclear suppressor system for a mitochondrial RNA polymerase mutant identifies an unusual zinc‐finger protein and a polyglutamine domain protein in Saccharomyces cerevisiae journal June 1994
Systematic measurement of transcription factor-DNA interactions by targeted mass spectrometry identifies candidate gene regulatory proteins journal February 2013
High expression of XYL2 coding for xylitol dehydrogenase is necessary for efficient xylose fermentation by engineered Saccharomyces cerevisiae journal July 2012
Luminescent nitrifying microorganisms patent May 1999
Yeast/E. coli shuttle vectors with multiple unique restriction sites journal September 1986
The Function and Properties of the Azf1 Transcriptional Regulator Change with Growth Conditions in Saccharomyces cerevisiae journal February 2006
Xylose isomerase overexpression along with engineering of the pentose phosphate pathway and evolutionary engineering enable rapid xylose utilization and ethanol production by Saccharomyces cerevisiae journal November 2012
Bacterial Xylose Isomerases Active in Yeast Cells patent-application May 2016
Transcriptional Profiling of Biofilm Regulators Identified by an Overexpression Screen in Saccharomyces cerevisiae journal August 2017
A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds journal April 2009
Method for the Production of Transgenic Plants with Increased Virus Resistance by Silencing Vegetable DNAJ-Like Proteins patent-application November 2005
Kinetic modelling reveals current limitations in the production of ethanol from xylose by recombinant Saccharomyces cerevisiae journal September 2011
Methods of increasing accumulation of essential amino acids in seeds patent October 2000
Correlation between microarray DNA hybridization efficiency and the position of short capture probe on the target nucleic acid journal July 2005
Systematic Genomic Library and Uses Thereof patent-application November 2010
Metabolic engineering of a xylose-isomerase-expressing strain for rapid anaerobic xylose fermentation journal February 2005