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Title: Single zymomonas mobilis strain for xylose and arabinose fermentation

Abstract

This invention relates to single microorganisms which normally do not ferment pentose sugars which are genetically altered to ferment the pentose sugars, xylose and arabinose, to produce ethanol, and a fermentation process utilizing the same. Examples include Zymomonas mobilis which has been transformed with a combination of E. coli genes for xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase. Expression of added genes are under the control of Z. mobilis promoters. These newly created microorganisms are useful for fermenting glucose, xylose and arabinose, produced by hydrolysis of hemicellulose and cellulose or starch, to produce ethanol.

Inventors:
 [1];  [2];  [3];  [4]
  1. (Lakewood, CO)
  2. (Wheat Ridge, CO)
  3. (Landenberg, PA)
  4. (Fort Collins, CO)
Issue Date:
Research Org.:
Midwest Research Institute
OSTI Identifier:
872008
Patent Number(s):
5843760
Assignee:
Midwest Research Institute (Kansas City, MI) NREL
DOE Contract Number:  
AC36-83CH10093
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
single; zymomonas; mobilis; strain; xylose; arabinose; fermentation; relates; microorganisms; normally; ferment; pentose; sugars; genetically; altered; produce; ethanol; process; utilizing; examples; transformed; combination; coli; genes; isomerase; xylulokinase; l-arabinose; l-ribulokinase; l-ribulose; 5-phosphate; 4-epimerase; transaldolase; transketolase; expression; added; control; z; promoters; newly; created; useful; fermenting; glucose; produced; hydrolysis; hemicellulose; cellulose; starch; xylose isomerase; pentose sugar; fermentation process; zymomonas mobilis; genetically altered; produce ethanol; process utilizing; coli genes; newly created; mobilis promoters; ferment pentose; added genes; pentose sugars; created microorganisms; l-arabinose isomerase; fermenting glucose; /435/999/

Citation Formats

Zhang, Min, Chou, Yat-Chen, Picataggio, Stephen K., and Finkelstein, Mark. Single zymomonas mobilis strain for xylose and arabinose fermentation. United States: N. p., 1998. Web.
Zhang, Min, Chou, Yat-Chen, Picataggio, Stephen K., & Finkelstein, Mark. Single zymomonas mobilis strain for xylose and arabinose fermentation. United States.
Zhang, Min, Chou, Yat-Chen, Picataggio, Stephen K., and Finkelstein, Mark. Thu . "Single zymomonas mobilis strain for xylose and arabinose fermentation". United States. https://www.osti.gov/servlets/purl/872008.
@article{osti_872008,
title = {Single zymomonas mobilis strain for xylose and arabinose fermentation},
author = {Zhang, Min and Chou, Yat-Chen and Picataggio, Stephen K. and Finkelstein, Mark},
abstractNote = {This invention relates to single microorganisms which normally do not ferment pentose sugars which are genetically altered to ferment the pentose sugars, xylose and arabinose, to produce ethanol, and a fermentation process utilizing the same. Examples include Zymomonas mobilis which has been transformed with a combination of E. coli genes for xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase. Expression of added genes are under the control of Z. mobilis promoters. These newly created microorganisms are useful for fermenting glucose, xylose and arabinose, produced by hydrolysis of hemicellulose and cellulose or starch, to produce ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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