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Title: Stable zymomonas mobilis xylose and arabinose fermenting strains

Abstract

The present invention briefly includes a transposon for stable insertion of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, and at least one promoter for expression of the structural genes in the bacterium, a pair of inverted insertion sequences, the operons contained inside the insertion sequences, and a transposase gene located outside of the insertion sequences. A plasmid shuttle vector for transformation of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, at least one promoter for expression of the structural genes in the bacterium, and at least two DNA fragments having homology with a gene in the bacterial genome to be transformed, is also provided.The transposon and shuttle vectors are useful in constructing significantly different Zymomonas mobilis strains, according to the present invention, which are useful in the conversion of the cellulose derived pentose sugars into fuels and chemicals, using traditional fermentation technology, because they are stable for expression in a non-selection medium.

Inventors:
 [1];  [2]
  1. Lakewood, CO
  2. Taipei, TW
Issue Date:
Research Org.:
Midwest Research Institute, Kansas City, MO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
936231
Patent Number(s):
7354755
Application Number:
09/565,233
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC36-98GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Zhang, Min, and Chou, Yat-Chen. Stable zymomonas mobilis xylose and arabinose fermenting strains. United States: N. p., 2008. Web.
Zhang, Min, & Chou, Yat-Chen. Stable zymomonas mobilis xylose and arabinose fermenting strains. United States.
Zhang, Min, and Chou, Yat-Chen. Tue . "Stable zymomonas mobilis xylose and arabinose fermenting strains". United States. https://www.osti.gov/servlets/purl/936231.
@article{osti_936231,
title = {Stable zymomonas mobilis xylose and arabinose fermenting strains},
author = {Zhang, Min and Chou, Yat-Chen},
abstractNote = {The present invention briefly includes a transposon for stable insertion of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, and at least one promoter for expression of the structural genes in the bacterium, a pair of inverted insertion sequences, the operons contained inside the insertion sequences, and a transposase gene located outside of the insertion sequences. A plasmid shuttle vector for transformation of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, at least one promoter for expression of the structural genes in the bacterium, and at least two DNA fragments having homology with a gene in the bacterial genome to be transformed, is also provided.The transposon and shuttle vectors are useful in constructing significantly different Zymomonas mobilis strains, according to the present invention, which are useful in the conversion of the cellulose derived pentose sugars into fuels and chemicals, using traditional fermentation technology, because they are stable for expression in a non-selection medium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {4}
}

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Works referenced in this record:

Transposon mutagenesis and strain construction in Zymomonas mobilis
journal, March 1997


Structural Analysis of Tn5
journal, January 1981


Fermentation Performance Assessment of a Genomically Integrated Xylose-Utilizing Recombinant of Zymomonas mobilis 39676
journal, January 2001


Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
journal, January 1995


Factors affecting transposition activity of IS50 and Tn5 ends
journal, March 1989


Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering.
journal, January 1996


Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli.
journal, January 1993