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Title: Stable zymomonas mobilis xylose and arabinose fermenting strains

Abstract

The present invention briefly includes a transposon for stable insertion of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, and at least one promoter for expression of the structural genes in the bacterium, a pair of inverted insertion sequences, the operons contained inside the insertion sequences, and a transposase gene located outside of the insertion sequences. A plasmid shuttle vector for transformation of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, at least one promoter for expression of the structural genes in the bacterium, and at least two DNA fragments having homology with a gene in the bacterial genome to be transformed, is also provided.The transposon and shuttle vectors are useful in constructing significantly different Zymomonas mobilis strains, according to the present invention, which are useful in the conversion of the cellulose derived pentose sugars into fuels and chemicals, using traditional fermentation technology, because they are stable for expression in a non-selection medium.

Inventors:
 [1];  [2]
  1. Lakewood, CO
  2. Taipei, TW
Issue Date:
Research Org.:
Midwest Research Institute (Kansas City, MO)
Sponsoring Org.:
USDOE
OSTI Identifier:
936231
Patent Number(s):
7,354,755
Application Number:
09/565,233
Assignee:
Midwest Research Institute (Kansas City, MO)
DOE Contract Number:  
AC36-98GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Zhang, Min, and Chou, Yat-Chen. Stable zymomonas mobilis xylose and arabinose fermenting strains. United States: N. p., 2008. Web.
Zhang, Min, & Chou, Yat-Chen. Stable zymomonas mobilis xylose and arabinose fermenting strains. United States.
Zhang, Min, and Chou, Yat-Chen. Tue . "Stable zymomonas mobilis xylose and arabinose fermenting strains". United States. https://www.osti.gov/servlets/purl/936231.
@article{osti_936231,
title = {Stable zymomonas mobilis xylose and arabinose fermenting strains},
author = {Zhang, Min and Chou, Yat-Chen},
abstractNote = {The present invention briefly includes a transposon for stable insertion of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, and at least one promoter for expression of the structural genes in the bacterium, a pair of inverted insertion sequences, the operons contained inside the insertion sequences, and a transposase gene located outside of the insertion sequences. A plasmid shuttle vector for transformation of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, at least one promoter for expression of the structural genes in the bacterium, and at least two DNA fragments having homology with a gene in the bacterial genome to be transformed, is also provided.The transposon and shuttle vectors are useful in constructing significantly different Zymomonas mobilis strains, according to the present invention, which are useful in the conversion of the cellulose derived pentose sugars into fuels and chemicals, using traditional fermentation technology, because they are stable for expression in a non-selection medium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {4}
}

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