Single zymomonas mobilis strain for xylose and arabinose fermentation
Abstract
This invention relates to single microorganisms which normally do not ferment pentose sugars which are genetically altered to ferment the pentose sugars, xylose and arabinose, to produce ethanol, and a fermentation process utilizing the same. Examples include Zymomonas mobilis which has been transformed with a combination of E. coli genes for xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase. Expression of added genes are under the control of Z. mobilis promoters. These newly created microorganisms are useful for fermenting glucose, xylose and arabinose, produced by hydrolysis of hemicellulose and cellulose or starch, to produce ethanol.
- Inventors:
-
- Lakewood, CO
- Wheat Ridge, CO
- Landenberg, PA
- Fort Collins, CO
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- OSTI Identifier:
- 872008
- Patent Number(s):
- 5843760
- Assignee:
- Midwest Research Institute (Kansas City, MI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC36-83CH10093
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- single; zymomonas; mobilis; strain; xylose; arabinose; fermentation; relates; microorganisms; normally; ferment; pentose; sugars; genetically; altered; produce; ethanol; process; utilizing; examples; transformed; combination; coli; genes; isomerase; xylulokinase; l-arabinose; l-ribulokinase; l-ribulose; 5-phosphate; 4-epimerase; transaldolase; transketolase; expression; added; control; z; promoters; newly; created; useful; fermenting; glucose; produced; hydrolysis; hemicellulose; cellulose; starch; xylose isomerase; pentose sugar; fermentation process; zymomonas mobilis; genetically altered; produce ethanol; process utilizing; coli genes; newly created; mobilis promoters; ferment pentose; added genes; pentose sugars; created microorganisms; l-arabinose isomerase; fermenting glucose; /435/999/
Citation Formats
Zhang, Min, Chou, Yat-Chen, Picataggio, Stephen K, and Finkelstein, Mark. Single zymomonas mobilis strain for xylose and arabinose fermentation. United States: N. p., 1998.
Web.
Zhang, Min, Chou, Yat-Chen, Picataggio, Stephen K, & Finkelstein, Mark. Single zymomonas mobilis strain for xylose and arabinose fermentation. United States.
Zhang, Min, Chou, Yat-Chen, Picataggio, Stephen K, and Finkelstein, Mark. Thu .
"Single zymomonas mobilis strain for xylose and arabinose fermentation". United States. https://www.osti.gov/servlets/purl/872008.
@article{osti_872008,
title = {Single zymomonas mobilis strain for xylose and arabinose fermentation},
author = {Zhang, Min and Chou, Yat-Chen and Picataggio, Stephen K and Finkelstein, Mark},
abstractNote = {This invention relates to single microorganisms which normally do not ferment pentose sugars which are genetically altered to ferment the pentose sugars, xylose and arabinose, to produce ethanol, and a fermentation process utilizing the same. Examples include Zymomonas mobilis which has been transformed with a combination of E. coli genes for xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase. Expression of added genes are under the control of Z. mobilis promoters. These newly created microorganisms are useful for fermenting glucose, xylose and arabinose, produced by hydrolysis of hemicellulose and cellulose or starch, to produce ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1998},
month = {Thu Jan 01 00:00:00 EST 1998}
}
Works referenced in this record:
The organization of the araBAD operon of Escherichia coli
journal, January 1986
- Lee, N.; Gielow, W.; Martin, R.
- Gene, Vol. 47, Issue 2-3
Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains
journal, December 1992
- Feldmann, SigrunD.; Sahm, Hermann; Sprenger, GeorgA.
- Applied Microbiology and Biotechnology, Vol. 38, Issue 3
Approaches to broaden the substrate and product range of the ethanologenic bacterium Zymomonas mobilis by genetic engineering
journal, February 1993
- Sprenger, Georg A.
- Journal of Biotechnology, Vol. 27, Issue 3
Molecular characterization of the Zymomonas mobilis enolase (eno) gene.
journal, January 1992
- Burnett, M. E.; Liu, J.; Conway, T.
- Journal of Bacteriology, Vol. 174, Issue 20