Device for detecting molecules, method for detecting molecules
Abstract
A construct is provided that is capable of binding a plurality of molecules, the construct comprising a first moiety with a first molecule binding region and a first molecule non-binding region; and a second moiety with a second molecule-binding region and a second molecule-non-binding region, whereby the first binding region and second binding region are at opposite ends of the construct. Also provided is a method for detecting protein having certain amino acid sequences, the method comprising supplying a collection of proteins each with unknown amino acid sequences, contacting the collection with a moiety having a plurality of binding sites capable of binding with the protein having certain amino acid sequences so as to form a moiety-protein complex, and mixing the complex with a marker specific for the moiety in an amount sufficient to indicate existence of the complex.
- Inventors:
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531624
- Patent Number(s):
- 7659083
- Application Number:
- 12/199,683
- Assignee:
- UChicago Argonne, LLC (Chicago, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2008-08-27
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Stevens, Fred J., Schiffer, Marianne, Wilkins-Stevens, Priscilla, Hanly, W. Carey, and Tollaksen, Sandra L. Device for detecting molecules, method for detecting molecules. United States: N. p., 2010.
Web.
Stevens, Fred J., Schiffer, Marianne, Wilkins-Stevens, Priscilla, Hanly, W. Carey, & Tollaksen, Sandra L. Device for detecting molecules, method for detecting molecules. United States.
Stevens, Fred J., Schiffer, Marianne, Wilkins-Stevens, Priscilla, Hanly, W. Carey, and Tollaksen, Sandra L. Tue .
"Device for detecting molecules, method for detecting molecules". United States. https://www.osti.gov/servlets/purl/1531624.
@article{osti_1531624,
title = {Device for detecting molecules, method for detecting molecules},
author = {Stevens, Fred J. and Schiffer, Marianne and Wilkins-Stevens, Priscilla and Hanly, W. Carey and Tollaksen, Sandra L.},
abstractNote = {A construct is provided that is capable of binding a plurality of molecules, the construct comprising a first moiety with a first molecule binding region and a first molecule non-binding region; and a second moiety with a second molecule-binding region and a second molecule-non-binding region, whereby the first binding region and second binding region are at opposite ends of the construct. Also provided is a method for detecting protein having certain amino acid sequences, the method comprising supplying a collection of proteins each with unknown amino acid sequences, contacting the collection with a moiety having a plurality of binding sites capable of binding with the protein having certain amino acid sequences so as to form a moiety-protein complex, and mixing the complex with a marker specific for the moiety in an amount sufficient to indicate existence of the complex.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 09 00:00:00 EST 2010},
month = {Tue Feb 09 00:00:00 EST 2010}
}
Works referenced in this record:
Structure of an antibody-antigen complex: crystal structure of the HyHEL-10 Fab-lysozyme complex.
journal, August 1989
- Padlan, E. A.; Silverton, E. W.; Sheriff, S.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 15
A domain flip as a result of a single amino-acid substitution
journal, August 1998
- Pokkuluri, Pr; Huang, D-B; Raffen, R.
- Structure, Vol. 6, Issue 8